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Refine your searchDisease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal / Sakha, B.M. in Nepal Agriculture Research Journal, Vol. 8 (2007)
Title : Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal Material Type: printed text Authors: Sakha, B.M., Author ; GP Rai, Author ; Dhital, S.P., Author Publication Date: 2007 Article on page: 7-13 p Languages : English (eng) Keywords: DAS-ELISA, meristem excision, potato, pre-basic seed, thermotherapy Abstract: Pre-basic seed potatoes are disease free potato minitubers produced by transplanting
pathogen free in vitro potato plantlets under protected condition in aphid-proof glasshouse
and/or screen house. Double antibody sand witched - enzyme linked immuno-sorbant assay
is used to test six major potato viruses, namely PLRV, PVS, PVX, PVY, PVA and PVM.
Thermotherapy cum meristem tip excision techniques are used to eliminate these viruses.
Virus free in vitro potato plantlets are rapidly propagated by single nodal cuttings on
modified MS media. For pre-basic seed production disease free in vitro potato plantlets are
transplanted in the sterile sand soil substrate under glasshouse and screen house, once in
autumn season and next in spring season. Since 1990, National Potato Research Program has
been producing about 200,000 pre-basic seeds annually. So far, PBS of 19 different
recommended and released potato cultivars has been produced. Till date 3,465,799 PBS had
been produced and 3,217,666 pre-basic seeds distributed to the different seed potato growers
groups, District Agriculture Development Offices, government farms/research stations, and
NGOs/INGOs for subsequent basic seed potato production. After establishment of tissue
culture facilities in National Potato Research Program, the productivity of potato has been
increased by 71% due to utilization of pre-basic seed potatoes.Link for e-copy: http://elibrary.narc.gov.np/?r=20
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 7-13 p[article] Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal [printed text] / Sakha, B.M., Author ; GP Rai, Author ; Dhital, S.P., Author . - 2007 . - 7-13 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 7-13 p
Keywords: DAS-ELISA, meristem excision, potato, pre-basic seed, thermotherapy Abstract: Pre-basic seed potatoes are disease free potato minitubers produced by transplanting
pathogen free in vitro potato plantlets under protected condition in aphid-proof glasshouse
and/or screen house. Double antibody sand witched - enzyme linked immuno-sorbant assay
is used to test six major potato viruses, namely PLRV, PVS, PVX, PVY, PVA and PVM.
Thermotherapy cum meristem tip excision techniques are used to eliminate these viruses.
Virus free in vitro potato plantlets are rapidly propagated by single nodal cuttings on
modified MS media. For pre-basic seed production disease free in vitro potato plantlets are
transplanted in the sterile sand soil substrate under glasshouse and screen house, once in
autumn season and next in spring season. Since 1990, National Potato Research Program has
been producing about 200,000 pre-basic seeds annually. So far, PBS of 19 different
recommended and released potato cultivars has been produced. Till date 3,465,799 PBS had
been produced and 3,217,666 pre-basic seeds distributed to the different seed potato growers
groups, District Agriculture Development Offices, government farms/research stations, and
NGOs/INGOs for subsequent basic seed potato production. After establishment of tissue
culture facilities in National Potato Research Program, the productivity of potato has been
increased by 71% due to utilization of pre-basic seed potatoes.Link for e-copy: http://elibrary.narc.gov.np/?r=20 Economics of Foundation Seed Production of Vegetable Crops at Agriculture Research Station Pahhribas / P. K. Maharjan ; T. R. Chapagain ; P. L. Karna ; D. Sah
Title : Economics of Foundation Seed Production of Vegetable Crops at Agriculture Research Station Pahhribas Material Type: printed text Authors: P. K. Maharjan, Author ; T. R. Chapagain, Author ; P. L. Karna, Author ; D. Sah, Author Pagination: 288-290 p. Languages : English (eng) Keywords: economic viability, foundation seed, 1:1 ratio, supernormal profit Abstract: A study was carried out to access the economic viability of foundation seed production of
vegetable crops at agricultural research station. The study was conducted at Agricultural
Research Station, Pakhribas Dhankuta including four major vegetable crops namely: radish
(40 days and Mino Early), pea (Sikkim local and J/ 1050), broad leaf mustard (Tanklunm
local and Marpha broad leaf) and French bean (Four season and Trisuli). The result of the
study revealed that foundation seed can be produced in 1:1 ratio except in peas. It is
concluded that in the proper management condition with 1:1 BIC ratio foundation seed
production is possible.Link for e-copy: http://elibrary.narc.gov.np/?r=516 Economics of Foundation Seed Production of Vegetable Crops at Agriculture Research Station Pahhribas [printed text] / P. K. Maharjan, Author ; T. R. Chapagain, Author ; P. L. Karna, Author ; D. Sah, Author . - [s.d.] . - 288-290 p.
Languages : English (eng)
Keywords: economic viability, foundation seed, 1:1 ratio, supernormal profit Abstract: A study was carried out to access the economic viability of foundation seed production of
vegetable crops at agricultural research station. The study was conducted at Agricultural
Research Station, Pakhribas Dhankuta including four major vegetable crops namely: radish
(40 days and Mino Early), pea (Sikkim local and J/ 1050), broad leaf mustard (Tanklunm
local and Marpha broad leaf) and French bean (Four season and Trisuli). The result of the
study revealed that foundation seed can be produced in 1:1 ratio except in peas. It is
concluded that in the proper management condition with 1:1 BIC ratio foundation seed
production is possible.Link for e-copy: http://elibrary.narc.gov.np/?r=516 E-copies
http://nkcs.org.np/narc/dl/?r=516URL
in Proceedings of the 28th National Winter Crops Workshops held at Lumle, 9-10 March 2011 / Giri, Y.P.
Title : Evaluation of some local and improved storage containers for lentil seed storage under ambient condition Material Type: printed text Authors: Upadhyay, I.P., Author ; N.H Ghimire, Author ; R.B Yadav, Author Pagination: 266-272 p. Languages : English (eng) Keywords: Lentil Seed, Storage Structures, Moisture Content, Germination, Insect Infestation Abstract: An experiment was conducted at Regional Agricultural Research Station, Nepalgunj in the years 2007 and 2009 with the objective to evaluate locally available storage structures for lentil seed storage. The experiment was laid out in completely randomized design with eight treatments and three replications. Storage structures (treatments) used for the experiment were: Ghyampo (500 mm high, diameter-200 mm), Cotton cloth bag (length-400mm, breadth-250mm), Fertilizer sack (length- 600mm, breadth-300 mm), Polylined jute bag (length-700mm, breadth-250 mm lined with 200 gauge plastic), plastic bags of 500 and I 000 gauge, Plastic jars (height-400 mm, diameter-200 mm) and Metal box (26 gauge, UB-250mm, height-400 mm with 100 mm diameter opening in the top center).....................
Link for e-copy: http://elibrary.narc.gov.np/?r=1854 Evaluation of some local and improved storage containers for lentil seed storage under ambient condition [printed text] / Upadhyay, I.P., Author ; N.H Ghimire, Author ; R.B Yadav, Author . - [s.d.] . - 266-272 p.
in Proceedings of the 28th National Winter Crops Workshops held at Lumle, 9-10 March 2011 / Giri, Y.P.
Languages : English (eng)
Keywords: Lentil Seed, Storage Structures, Moisture Content, Germination, Insect Infestation Abstract: An experiment was conducted at Regional Agricultural Research Station, Nepalgunj in the years 2007 and 2009 with the objective to evaluate locally available storage structures for lentil seed storage. The experiment was laid out in completely randomized design with eight treatments and three replications. Storage structures (treatments) used for the experiment were: Ghyampo (500 mm high, diameter-200 mm), Cotton cloth bag (length-400mm, breadth-250mm), Fertilizer sack (length- 600mm, breadth-300 mm), Polylined jute bag (length-700mm, breadth-250 mm lined with 200 gauge plastic), plastic bags of 500 and I 000 gauge, Plastic jars (height-400 mm, diameter-200 mm) and Metal box (26 gauge, UB-250mm, height-400 mm with 100 mm diameter opening in the top center).....................
Link for e-copy: http://elibrary.narc.gov.np/?r=1854 E-copies
http://nkcs.org.np/narc/dl/?r=1854URL
in Proceedings of the fifth National Seminar on Horticulture June 9-10, 2008 / Nepal Agricultural Research Council (NARC) (Khumaltar, Lalitpur, Nepal)
Title : Evaluation of True Potato Seed Families and Us Adaptability in Nepal Material Type: printed text Authors: Ram C. Adhikari, Author ; Janardan Ghimire, Author ; Gyan P. Rai, Author ; Shambhu P. Baral, Author ; Duryodhan Chaudhary, Author ; K.C.Upreti, Author Pagination: 151-161 p. Languages : English (eng) Keywords: family, nursery bed, seedling tuber, true potato seed, yield Abstract: The major constraints for production of potatoes in Nepal are the poor quality and high
cost of seed tubers. Most successful and widely adoptable methodology in TPS has
been the use of seedling ·tubers for raising commercial crop. Twenty TPS families
received from International Potato Center Lima Peru were evaluated at National Potato
Research Program, Khumaltar, Agriculture Research Station Belachapi and Agriculture ·
Research Station Malepatan during 2005 and 2006 to see their performance. The
families were tested . in randomized complete block design (RCBD) with three
replications. TPS family C95 LB 22.2 x TP$ 67 and HPS IV67 significantly .produced
the maximum tuber yield 5.4 kg/rrf and 7. 03 kg/rrf at Khumaltar and Belachapi,
respectively in the nursery beds in 2005. But, in 2006 significantly maximum
seedling tuber yield 7.90 kglrrf, 5.04 kg/rrf and 4. 76 kg/rrf w~s obtained from
C96H 10.25 x TPS 13, FLS 15 x TPS 67 and FLS 12 x TPS 67 at Khuma/tar,
Malepatan and Belachapi, respectively. In commercial crop, F1C1 tuberlets or
C96H 13.29 x TPS 67 and FLS 12 x TPS 13 significantly produced the m_aximum tuber
yield (20.73 kg/7.2 rrf and 14.02 kg/7.2 rrf) at Khumaltar and Belachapi, respectively
as compared to Desiree (12. 17 kg/7.2 rrf at Khumaltar) and Lalgulab {5.87 kg/7.2 rr( at
Belachapi). Based on overall performance the results· suggest that the. hybrid TPS
families produce equal. or higher tuber yield as compared to standard clonal varieties.Link for e-copy: http://elibrary.narc.gov.np/?r=239 Evaluation of True Potato Seed Families and Us Adaptability in Nepal [printed text] / Ram C. Adhikari, Author ; Janardan Ghimire, Author ; Gyan P. Rai, Author ; Shambhu P. Baral, Author ; Duryodhan Chaudhary, Author ; K.C.Upreti, Author . - [s.d.] . - 151-161 p.
in Proceedings of the fifth National Seminar on Horticulture June 9-10, 2008 / Nepal Agricultural Research Council (NARC) (Khumaltar, Lalitpur, Nepal)
Languages : English (eng)
Keywords: family, nursery bed, seedling tuber, true potato seed, yield Abstract: The major constraints for production of potatoes in Nepal are the poor quality and high
cost of seed tubers. Most successful and widely adoptable methodology in TPS has
been the use of seedling ·tubers for raising commercial crop. Twenty TPS families
received from International Potato Center Lima Peru were evaluated at National Potato
Research Program, Khumaltar, Agriculture Research Station Belachapi and Agriculture ·
Research Station Malepatan during 2005 and 2006 to see their performance. The
families were tested . in randomized complete block design (RCBD) with three
replications. TPS family C95 LB 22.2 x TP$ 67 and HPS IV67 significantly .produced
the maximum tuber yield 5.4 kg/rrf and 7. 03 kg/rrf at Khumaltar and Belachapi,
respectively in the nursery beds in 2005. But, in 2006 significantly maximum
seedling tuber yield 7.90 kglrrf, 5.04 kg/rrf and 4. 76 kg/rrf w~s obtained from
C96H 10.25 x TPS 13, FLS 15 x TPS 67 and FLS 12 x TPS 67 at Khuma/tar,
Malepatan and Belachapi, respectively. In commercial crop, F1C1 tuberlets or
C96H 13.29 x TPS 67 and FLS 12 x TPS 13 significantly produced the m_aximum tuber
yield (20.73 kg/7.2 rrf and 14.02 kg/7.2 rrf) at Khumaltar and Belachapi, respectively
as compared to Desiree (12. 17 kg/7.2 rrf at Khumaltar) and Lalgulab {5.87 kg/7.2 rr( at
Belachapi). Based on overall performance the results· suggest that the. hybrid TPS
families produce equal. or higher tuber yield as compared to standard clonal varieties.Link for e-copy: http://elibrary.narc.gov.np/?r=239 E-copies
http://nkcs.org.np/narc/dl/?r=239URL
Title : Evaluation of True Potato Seed Families for Seedling Tuber Production in Nepal Material Type: printed text Authors: Adhikari, R.C., Author ; G. P. Rai, Author ; K.C.Upreti, Author ; L. P. Gautam,, Author ; Chaudhary, D.K., Author ; T. R. Chapagain, Author ; K. B. Bhandari, Author Pagination: 158-166 p. Languages : English (eng) Keywords: family, seedling tuber, true potato seed, tuber yield. Abstract: Seven hybrid true potato seed (TPS) families received from the International Potato Center,
Lima, Peru were evaluated and compared with HPS 11167 and HPS 7167 during 2001 to 2003
to see the performance of hybrid TPS families for seedling tuber p_roduction in di.fferellt agroecological
zones of Nepal. The families were tested in randomized complete block design
(RCBD) with four replications. Tuber yield produced by MF II x IPS 67 (5.12 kg/m2
) and
Atzimba x TPS 67 (3.15 kg!nl) at Khumaltar and Lumle, respectively were respectively
higher than other tested families. In Pakhribas, Surkhet and Taralu:;ra maximum tuber
weights were obtainedfromMF II x TPS 67 (3.94 kg/m2
), Serranax TPS 13 (2.24 kg!nl) and
HPS 11167 (6.20 kglm2
). Atzimba x TPS 67 produced significalltly maximum tuber number at
Khumaltar (496.47/nh Lumle (400.85/m2
) and Pakftribas conditions (349.01/m2
), whereas
in Surkhet and Tarahara, maximum tuber number were recorded in Serrana x TPS 13
(352.05/m2
) and HPS 11167 (723.67/m2
), "respectively. The average tuber weight among the
families were statistically significant, Maximum average tuber weights were recorded in LT 8
x TPS 13 in all the locations. None of the families were resistant/tolerance to late blight
(Phytophhora infestans) disease at Lumle. Based on overall performance, TPS families MF II
x TPS 67, Atzimba x TPS 67, MF II TPS 67 and HPS 7167, Serrano x TPS 13, MF II x TPS 67
and HPS 11167 were found promising for seedling tuber production in Lumle, Pakhribas,
Surkhet and Tarahara conditions, respectively.Link for e-copy: http://elibrary.narc.gov.np/?r=484 Evaluation of True Potato Seed Families for Seedling Tuber Production in Nepal [printed text] / Adhikari, R.C., Author ; G. P. Rai, Author ; K.C.Upreti, Author ; L. P. Gautam,, Author ; Chaudhary, D.K., Author ; T. R. Chapagain, Author ; K. B. Bhandari, Author . - [s.d.] . - 158-166 p.
Languages : English (eng)
Keywords: family, seedling tuber, true potato seed, tuber yield. Abstract: Seven hybrid true potato seed (TPS) families received from the International Potato Center,
Lima, Peru were evaluated and compared with HPS 11167 and HPS 7167 during 2001 to 2003
to see the performance of hybrid TPS families for seedling tuber p_roduction in di.fferellt agroecological
zones of Nepal. The families were tested in randomized complete block design
(RCBD) with four replications. Tuber yield produced by MF II x IPS 67 (5.12 kg/m2
) and
Atzimba x TPS 67 (3.15 kg!nl) at Khumaltar and Lumle, respectively were respectively
higher than other tested families. In Pakhribas, Surkhet and Taralu:;ra maximum tuber
weights were obtainedfromMF II x TPS 67 (3.94 kg/m2
), Serranax TPS 13 (2.24 kg!nl) and
HPS 11167 (6.20 kglm2
). Atzimba x TPS 67 produced significalltly maximum tuber number at
Khumaltar (496.47/nh Lumle (400.85/m2
) and Pakftribas conditions (349.01/m2
), whereas
in Surkhet and Tarahara, maximum tuber number were recorded in Serrana x TPS 13
(352.05/m2
) and HPS 11167 (723.67/m2
), "respectively. The average tuber weight among the
families were statistically significant, Maximum average tuber weights were recorded in LT 8
x TPS 13 in all the locations. None of the families were resistant/tolerance to late blight
(Phytophhora infestans) disease at Lumle. Based on overall performance, TPS families MF II
x TPS 67, Atzimba x TPS 67, MF II TPS 67 and HPS 7167, Serrano x TPS 13, MF II x TPS 67
and HPS 11167 were found promising for seedling tuber production in Lumle, Pakhribas,
Surkhet and Tarahara conditions, respectively.Link for e-copy: http://elibrary.narc.gov.np/?r=484 E-copies
http://nkcs.org.np/narc/dl/?r=484URL
