4 result(s) search for keyword(s) 'cultivars'    

Classification of Nepalese rice cultivars based on Isozymes / B.K. Joshi    

Public ISBD in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.     Title : Classification of Nepalese rice cultivars based on Isozymes Material Type: printed text Authors: B.K. Joshi ; H.P Bimb, Author Pagination: 175-187 p. Languages : English (eng) Keywords: Varietal  classification  ;  isozyme  rice  cultivars Abstract: Classification of large variation within Oryza sativa has been importance for breeders and geneticists. A total of 39 Nepalese rice cultivars including one non-released, Fuji-102 were classified and evaluated their characteristics features of eight isozyme systems, Alcohol dehydrogenase (ADH), Acid phosphatase (ACP), Esterase (EST), lsocitrate dehydrogenase (IDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Peroxidase (POX) and Shikimate dehydrogenase (SDH). Plumules and coleoptiles of 3-5 seedlings of each cultivar were used to extract enzyme tfSing Sodium ascorbate extraction buffer at pH 7.4. These were electrophoresed and enzymes were detected after staining in an appropriate staining mixture. Cluster and principal component analyses were applied on binary data matrix. Zymograms were presented separately for each enzyme and each varietal type. A to tal of 28, 19 and 23 distinct electromorphs were used as iso:;ymic descriptors for indica, intermediate and japonica varietal types respectively. Multivariate analysis grouped these cultivars into 3. These were reclassified in indica (22), intermediate (3} andjaponica (14) varietal types. There were sixteen loci coding eight isozyme systems. All loci were f ound in indica group, /4 loci in intermediate and 15 in japonica. Most of the enzyme systems expressed distinct zymogramsfor indica andjaponica. Highest zymotypes were expressed by POX Loci Mdh-3 and Sdh-1 have highest number of alleles within indica type, Pox-] within intermediate and Sdh-1 and Pox-/ withinjaponica types. Locus Mdh-2 was not noticed in intermediate andjaponica groups. Loci Mdh-3, Est-2 and Sdh-1 .showed hfg~est gene diversity within indica, intermediate and j aponica rypes respectively. Among the four multilocus -enzymes, three were polymorphic. All loci except Pox-4 were polymorhpic in intermediate varietal types. all loci were polymorhpihc in indica and except Pox-3 and Adh:-1 all loci were polymorphic considering null allele injaponica. This isozymic variation within and between varietal types can be itseful for further study and the isozyme profiles of each cultivar can be used to trace their identity. Link for e-copy: http://elibrary.narc.gov.np/?r=1100 in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.     Classification of Nepalese rice cultivars based on Isozymes [printed text] / B.K. Joshi ; H.P Bimb, Author . - [s.d.] . - 175-187 p. Languages : English (eng) Keywords: Varietal  classification  ;  isozyme  rice  cultivars Abstract: Classification of large variation within Oryza sativa has been importance for breeders and geneticists. A total of 39 Nepalese rice cultivars including one non-released, Fuji-102 were classified and evaluated their characteristics features of eight isozyme systems, Alcohol dehydrogenase (ADH), Acid phosphatase (ACP), Esterase (EST), lsocitrate dehydrogenase (IDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Peroxidase (POX) and Shikimate dehydrogenase (SDH). Plumules and coleoptiles of 3-5 seedlings of each cultivar were used to extract enzyme tfSing Sodium ascorbate extraction buffer at pH 7.4. These were electrophoresed and enzymes were detected after staining in an appropriate staining mixture. Cluster and principal component analyses were applied on binary data matrix. Zymograms were presented separately for each enzyme and each varietal type. A to tal of 28, 19 and 23 distinct electromorphs were used as iso:;ymic descriptors for indica, intermediate and japonica varietal types respectively. Multivariate analysis grouped these cultivars into 3. These were reclassified in indica (22), intermediate (3} andjaponica (14) varietal types. There were sixteen loci coding eight isozyme systems. All loci were f ound in indica group, /4 loci in intermediate and 15 in japonica. Most of the enzyme systems expressed distinct zymogramsfor indica andjaponica. Highest zymotypes were expressed by POX Loci Mdh-3 and Sdh-1 have highest number of alleles within indica type, Pox-] within intermediate and Sdh-1 and Pox-/ withinjaponica types. Locus Mdh-2 was not noticed in intermediate andjaponica groups. Loci Mdh-3, Est-2 and Sdh-1 .showed hfg~est gene diversity within indica, intermediate and j aponica rypes respectively. Among the four multilocus -enzymes, three were polymorphic. All loci except Pox-4 were polymorhpic in intermediate varietal types. all loci were polymorhpihc in indica and except Pox-3 and Adh:-1 all loci were polymorphic considering null allele injaponica. This isozymic variation within and between varietal types can be itseful for further study and the isozyme profiles of each cultivar can be used to trace their identity. Link for e-copy: http://elibrary.narc.gov.np/?r=1100

E-copies

http://nkcs.org.np/narc/dl/?r=1100 URL

Genetic Diversity in Nepalese Wheat Cultivars Based on Agro-Morphological Traits and Coefficients of Parentage / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 5 (2004)  

Public ISBD [article]  inNepal Agriculture Research Journal > Vol. 5 (2004) . - 7-17 p Title : Genetic Diversity in Nepalese Wheat Cultivars Based on Agro-Morphological Traits and Coefficients of Parentage Material Type: printed text Authors: Joshi, Bal K., Author ; Ashok Mudwari, Author ; Bhatta, M.R., Author Publication Date: 2004 Article on page: 7-17 p Languages : English (eng) Keywords: Agromorphological  traits,  coefficient  of  parentage,  genetic  diversity,  Nepalese  wheat  cultivars Abstract: Genetic diversity between parents is necessary to derive transgenic segregants from a cross. Coefficient of parentage (COP) and agromorphological traits (AMT) can be used to estimate diversity among cultivars. The pedigrees of 26 bread wheat cultivars were traced back to 77 ancestors and computed coefficient of parentage for all pair-wise combinations. All the cultivars used in the pedigree analysis were evaluated for six agromorphological traits in the National Wheat Research Programme (NWRP), Bhairahawa, Nepal in 1996. Six quantitative variables were used to compute dissimilarity distance matrix. Cluster and principal components analyses were performed on the matrix of COP and AMT values. COP matrix and the matrix based on agromorphological traits were compared. Mexico, India and Nepal were countries of the origin for 26 cultivars. A total of 77 ancestors originated from 22 different countries were used to develop these cultivars. Most of the ancestors were aestivum (80.52%) and spring growth habit (64.94%). Maximum dissimilarity was between RR 21 and Annapurna 3 and the most closely related pair was Rohini and BL 1022 based on the AMT. The mean of COP for all cultivars was 0.159 0.256. The highest COP was between Annapurna 3 and Annapurna 2. Other more closely related pairs based on COP were Kalyansona and Annapurna 2, Pasang Lhamu and Annapurna 3, UP 262 and RR 21, Vaskar and Kalyansona, NL 297 and BL 1473, Pasang Lhamu and Annapurna 1. Completely unrelated pairs were L 52 and HD 1982, L 52 and Kalyansona, LR 64 and Kalyansona, Kalyansona and HD 1982, PI and Kalyansona, PI and L 52, RR 21 and HD 1982, RR 21 and Kalyansona, RR 21 and PI. Fifteen ancestors were present in at least about 65% of the cultivars. 17 ancestors had been used more frequently. Five and six clusters were formed based on AMT and COP, respectively. Correlation coefficient between COP and AMT was 0.18 (P = 0.0168). Cultivars surveyed represent a wide range of variation for different areas of origin and adaptation. This genetic variation may be useful for further improvement of wheat and it is necessary to conserve them. Link for e-copy: http://elibrary.narc.gov.np/?r=72 [article] Genetic Diversity in Nepalese Wheat Cultivars Based on Agro-Morphological Traits and Coefficients of Parentage [printed text] / Joshi, Bal K., Author ; Ashok Mudwari, Author ; Bhatta, M.R., Author . - 2004 . - 7-17 p. Languages : English (eng) in Nepal Agriculture Research Journal > Vol. 5 (2004) . - 7-17 p Keywords: Agromorphological  traits,  coefficient  of  parentage,  genetic  diversity,  Nepalese  wheat  cultivars Abstract: Genetic diversity between parents is necessary to derive transgenic segregants from a cross. Coefficient of parentage (COP) and agromorphological traits (AMT) can be used to estimate diversity among cultivars. The pedigrees of 26 bread wheat cultivars were traced back to 77 ancestors and computed coefficient of parentage for all pair-wise combinations. All the cultivars used in the pedigree analysis were evaluated for six agromorphological traits in the National Wheat Research Programme (NWRP), Bhairahawa, Nepal in 1996. Six quantitative variables were used to compute dissimilarity distance matrix. Cluster and principal components analyses were performed on the matrix of COP and AMT values. COP matrix and the matrix based on agromorphological traits were compared. Mexico, India and Nepal were countries of the origin for 26 cultivars. A total of 77 ancestors originated from 22 different countries were used to develop these cultivars. Most of the ancestors were aestivum (80.52%) and spring growth habit (64.94%). Maximum dissimilarity was between RR 21 and Annapurna 3 and the most closely related pair was Rohini and BL 1022 based on the AMT. The mean of COP for all cultivars was 0.159 0.256. The highest COP was between Annapurna 3 and Annapurna 2. Other more closely related pairs based on COP were Kalyansona and Annapurna 2, Pasang Lhamu and Annapurna 3, UP 262 and RR 21, Vaskar and Kalyansona, NL 297 and BL 1473, Pasang Lhamu and Annapurna 1. Completely unrelated pairs were L 52 and HD 1982, L 52 and Kalyansona, LR 64 and Kalyansona, Kalyansona and HD 1982, PI and Kalyansona, PI and L 52, RR 21 and HD 1982, RR 21 and Kalyansona, RR 21 and PI. Fifteen ancestors were present in at least about 65% of the cultivars. 17 ancestors had been used more frequently. Five and six clusters were formed based on AMT and COP, respectively. Correlation coefficient between COP and AMT was 0.18 (P = 0.0168). Cultivars surveyed represent a wide range of variation for different areas of origin and adaptation. This genetic variation may be useful for further improvement of wheat and it is necessary to conserve them. Link for e-copy: http://elibrary.narc.gov.np/?r=72

Modeling the sensitivity of CERES-Rice model: An experience of Nepal / A. Lamsal in Agronomy Journal of Nepal, Vol.3 (2013)  

Public ISBD [article]  inAgronomy Journal of Nepal > Vol.3 (2013) . - 11-22p. Title : Modeling the sensitivity of CERES-Rice model: An experience of Nepal Material Type: printed text Authors: A. Lamsal, Author ; LP Amgai, Author ; A. Giri, Author Publication Date: 2013 Article on page: 11-22p. Languages : English (eng) Keywords: CERES-Rice,  Sensitivity,  Yield,  and  Cultivars Link for e-copy: http://elibrary.narc.gov.np/?r=1318 [article] Modeling the sensitivity of CERES-Rice model: An experience of Nepal [printed text] / A. Lamsal, Author ; LP Amgai, Author ; A. Giri, Author . - 2013 . - 11-22p. Languages : English (eng) in Agronomy Journal of Nepal > Vol.3 (2013) . - 11-22p. Keywords: CERES-Rice,  Sensitivity,  Yield,  and  Cultivars Link for e-copy: http://elibrary.narc.gov.np/?r=1318

Variability in Phase Transition and Contribution of Genetic Variance to Yield in Wheat at High Altitude / Barid B Bandyopadhyay in Nepal Agriculture Research Journal, Vol.11 (2011)  

Public ISBD [article]  inNepal Agriculture Research Journal > Vol.11 (2011) . - 21-27p. Title : Variability in Phase Transition and Contribution of Genetic Variance to Yield in Wheat at High Altitude Material Type: printed text Authors: Barid B Bandyopadhyay, Author Publication Date: 2011 Article on page: 21-27p. Languages : English (eng) Keywords: High  altitude,  Low  temperature,  Water  deficit  stress,  Wheat  cultivars Link for e-copy: http://elibrary.narc.gov.np/?r=1190 [article] Variability in Phase Transition and Contribution of Genetic Variance to Yield in Wheat at High Altitude [printed text] / Barid B Bandyopadhyay, Author . - 2011 . - 21-27p. Languages : English (eng) in Nepal Agriculture Research Journal > Vol.11 (2011) . - 21-27p. Keywords: High  altitude,  Low  temperature,  Water  deficit  stress,  Wheat  cultivars Link for e-copy: http://elibrary.narc.gov.np/?r=1190