Search results
4 result(s) search for keyword(s) 'buckwheat,' 
Refine your searchBuckwheat diversity in Nepal in the context of morphological, biochemical and Molecular analysis / H.P Bimb
in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.
Title : Buckwheat diversity in Nepal in the context of morphological, biochemical and Molecular analysis Material Type: printed text Authors: H.P Bimb, Author Pagination: 146 p. Languages : English (eng) Keywords: Biochemical, buckwheat, diversity, molecular analysis, morphology, Nepal Abstract: Landraces and wild relative of crop species are regarded as the indispensable gene sources for future genetic improvement. For effective conservation of genetic variation, it is necessary to identify the target population based on its genetic variation and sustainability in its habitat. Buckwheat (Fagopyrum spp) being grown in wide geographic variations of Nepal is especially important source of food for marginal farmers of hills and mountains………..
Link for e-copy: http://elibrary.narc.gov.np/?r=1076 Buckwheat diversity in Nepal in the context of morphological, biochemical and Molecular analysis [printed text] / H.P Bimb, Author . - [s.d.] . - 146 p.
in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.
Languages : English (eng)
Keywords: Biochemical, buckwheat, diversity, molecular analysis, morphology, Nepal Abstract: Landraces and wild relative of crop species are regarded as the indispensable gene sources for future genetic improvement. For effective conservation of genetic variation, it is necessary to identify the target population based on its genetic variation and sustainability in its habitat. Buckwheat (Fagopyrum spp) being grown in wide geographic variations of Nepal is especially important source of food for marginal farmers of hills and mountains………..
Link for e-copy: http://elibrary.narc.gov.np/?r=1076 E-copies
http://nkcs.org.np/narc/dl/?r=1076URLCommon Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 7 (2006)
Title : Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum Material Type: printed text Authors: Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; Ryo Ohsawa, Author Publication Date: 2006 Article on page: 27-36 p Languages : English (eng) Keywords: Common buckwheat, EST markers, Fagopyrum species, transferability Abstract: If the EST primers designed for one species can be used in related species, then the cost involved
in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for
other species is significantly reduced. We tested the applicability of 17 EST primers developed
from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18
accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones
were used to design primers using Primer3. Amplification products were different in band
intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All
primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced
very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight
primers amplified the DNA of all accessions. Results indicated that the transferability of EST
markers developed for common buckwheat decreased with an increase in genetic distance
between them.Link for e-copy: http://elibrary.narc.gov.np/?r=43
in Nepal Agriculture Research Journal > Vol. 7 (2006) . - 27-36 p[article] Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum [printed text] / Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; Ryo Ohsawa, Author . - 2006 . - 27-36 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 7 (2006) . - 27-36 p
Keywords: Common buckwheat, EST markers, Fagopyrum species, transferability Abstract: If the EST primers designed for one species can be used in related species, then the cost involved
in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for
other species is significantly reduced. We tested the applicability of 17 EST primers developed
from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18
accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones
were used to design primers using Primer3. Amplification products were different in band
intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All
primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced
very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight
primers amplified the DNA of all accessions. Results indicated that the transferability of EST
markers developed for common buckwheat decreased with an increase in genetic distance
between them.Link for e-copy: http://elibrary.narc.gov.np/?r=43
Title : Optimization of PCR Conditions for DNA Amplification of Common Buckwheat Using EST Primers Material Type: printed text Authors: Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; T. Hara, Author Publication Date: 2007 Article on page: 1-6 p. Languages : English (eng) Keywords: Annealing temperature, buckwheat, EST markers, optimization of PCRconditions Abstract: Under optimal conditions the PCR reaction is very efficient; microgram quantities may be
synthesized from a single molecule of substrate DNA. DNA of four lines of common
buckwheat (Kyusu, Canada, Miyazaki and Botansoba) was used to optimize PCR reaction
and cycling program of 26 primers for DNA amplification of common buckwheat.
Annealing temperature (Ta), PCR cycle number and MgCl2 concentration were considered
optimum if the single clear band was observed. Of the 26 primers Ta of only 10 primers
could be optimized. Three primer pairs performed best at Ta of 54oC. The optimum
concentration of MgCl2 was found to be 1.5mM for all primer pairs. Similarly the number of
PCR cycles was found to be 40 for all 10 primer pairs except for primer pair 57. Optimized
PCR conditions were used for subsequent studies such as transferability of EST primers to
other Fagopyrum species and construction of linkage map.Link for e-copy: http://elibrary.narc.gov.np/?r=17
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 1-6 p.[article] Optimization of PCR Conditions for DNA Amplification of Common Buckwheat Using EST Primers [printed text] / Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; T. Hara, Author . - 2007 . - 1-6 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 1-6 p.
Keywords: Annealing temperature, buckwheat, EST markers, optimization of PCRconditions Abstract: Under optimal conditions the PCR reaction is very efficient; microgram quantities may be
synthesized from a single molecule of substrate DNA. DNA of four lines of common
buckwheat (Kyusu, Canada, Miyazaki and Botansoba) was used to optimize PCR reaction
and cycling program of 26 primers for DNA amplification of common buckwheat.
Annealing temperature (Ta), PCR cycle number and MgCl2 concentration were considered
optimum if the single clear band was observed. Of the 26 primers Ta of only 10 primers
could be optimized. Three primer pairs performed best at Ta of 54oC. The optimum
concentration of MgCl2 was found to be 1.5mM for all primer pairs. Similarly the number of
PCR cycles was found to be 40 for all 10 primer pairs except for primer pair 57. Optimized
PCR conditions were used for subsequent studies such as transferability of EST primers to
other Fagopyrum species and construction of linkage map.Link for e-copy: http://elibrary.narc.gov.np/?r=17
Title : Green house studies on the biology of winter annual grasses and the use of cover crops and herbicides for their control Material Type: printed text Authors: Ranjit, Jagat Devi, Author ; R Bcllinder,, Author ; C Benidict, Author ; V Kumar, Author Publication Date: 2011 Article on page: 139-148p. Languages : English (eng) Keywords: Grass, Herbicides, Buckwheat, Residues Link for e-copy: http://elibrary.narc.gov.np/?r=1278
in Agronomy Journal of Nepal > Vol.2 (2011) . - 139-148p.[article] Green house studies on the biology of winter annual grasses and the use of cover crops and herbicides for their control [printed text] / Ranjit, Jagat Devi, Author ; R Bcllinder,, Author ; C Benidict, Author ; V Kumar, Author . - 2011 . - 139-148p.
Languages : English (eng)
in Agronomy Journal of Nepal > Vol.2 (2011) . - 139-148p.
Keywords: Grass, Herbicides, Buckwheat, Residues Link for e-copy: http://elibrary.narc.gov.np/?r=1278
