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in Proceedings of the 27th National Summer Crops Workshop, held at Rampur, Chitawan, 18-20 April 2013, Vol. I / Giri, Y.P.
Title : Evaluation of rice genotypes against blast disease under field condition Material Type: printed text Authors: Nabin Kumar Dangal ; Bedanand Chaudhary, Author ; Parbati Joshi, Author ; Ang Tenjing Sherpa, Author Pagination: 323-330 p. Languages : English (eng) Keywords: Pyricularia grise a, genotype, disease severity, leaf blast and resist~ce Abstract: Blast of rice, caused by Pyricularia grisea, is one of the most important diseases of rice in
Nepal. It has been causing significant yield loss in all rice growing areas. A field screening
experiment was conducted to identify resistance in rice to the disease during 2011 and 2012
wet season at Regional Agricultural Research Station, Tarahara. A total of 134 (OBN -70,
RA VTRL-26, RA VTN-22 and Quality-16) rice genotypes were evaluated for resistance in
both the years. atural spread of the pathogen in the test lines was allowed from border lines I
inoculums rows planted around the nursery with mixture of susceptible varieties (i.e. Mansuli
and Sankharika). Conducive environment for blast development was created by planting four
rows of Sesbania eculeata around the experimental plot 35 days prior to seeding of test
genotypes. Scoring was done on 22 days after seeding and continued for three scorings at 7
days interval using 0-9 scale developed by IRRI. Area under disease progress curve (AUDPC)
varied significantly among the tested rice genotypes in both years. Disease severity varied 0 to
77.77%. Out of 134 entries, 58 entries were resistant to the disease during 2011 and 63 in
2012. Among them, IR09Fl12, IR09Fl20, IR09Fl27, IR09F158, IR09Fl66, IR09Fl74,
IR09F 178, TR09F226, IR09F229, IR09F236, IR09F238, R09F253, IR09F293, IR-64-Sub-1,
IRRI-119, lET 14846, WAS 202-B-B-13 , IR 75299-94-1-2-2, IR 73007-131-1-2-2, 1R 70215-
70-CPA3-4-1-3, IR 80411-B-28-4, IR 79913-B-176-B-4, IR 78937-B-20-B-B-4, BR 4363-3-
8-1-2-4(BR-32), Basundhara, Tarahara-1, Hardinath-2, Sura vi and Mahima were free from
blast in both the years. The number of moderately resistant (3 -4), moderately susceptible (5 -6)
and highly susceptible (7-9) genotypes was 43, 9 and 2 in 2011 and 54, 3 and 1 in 2012,
respectively. Disease severity increased in second and third scorings in all entries except the
resistant ones. IR09Fll0, NR 1887-8-1-1-2 and Kanchhi masuli were highly susceptible to
blast with the highest disease score (7) which were completely knocked down in the final
scoring.Link for e-copy: http://elibrary.narc.gov.np/?r=919 Evaluation of rice genotypes against blast disease under field condition [printed text] / Nabin Kumar Dangal ; Bedanand Chaudhary, Author ; Parbati Joshi, Author ; Ang Tenjing Sherpa, Author . - [s.d.] . - 323-330 p.
in Proceedings of the 27th National Summer Crops Workshop, held at Rampur, Chitawan, 18-20 April 2013, Vol. I / Giri, Y.P.
Languages : English (eng)
Keywords: Pyricularia grise a, genotype, disease severity, leaf blast and resist~ce Abstract: Blast of rice, caused by Pyricularia grisea, is one of the most important diseases of rice in
Nepal. It has been causing significant yield loss in all rice growing areas. A field screening
experiment was conducted to identify resistance in rice to the disease during 2011 and 2012
wet season at Regional Agricultural Research Station, Tarahara. A total of 134 (OBN -70,
RA VTRL-26, RA VTN-22 and Quality-16) rice genotypes were evaluated for resistance in
both the years. atural spread of the pathogen in the test lines was allowed from border lines I
inoculums rows planted around the nursery with mixture of susceptible varieties (i.e. Mansuli
and Sankharika). Conducive environment for blast development was created by planting four
rows of Sesbania eculeata around the experimental plot 35 days prior to seeding of test
genotypes. Scoring was done on 22 days after seeding and continued for three scorings at 7
days interval using 0-9 scale developed by IRRI. Area under disease progress curve (AUDPC)
varied significantly among the tested rice genotypes in both years. Disease severity varied 0 to
77.77%. Out of 134 entries, 58 entries were resistant to the disease during 2011 and 63 in
2012. Among them, IR09Fl12, IR09Fl20, IR09Fl27, IR09F158, IR09Fl66, IR09Fl74,
IR09F 178, TR09F226, IR09F229, IR09F236, IR09F238, R09F253, IR09F293, IR-64-Sub-1,
IRRI-119, lET 14846, WAS 202-B-B-13 , IR 75299-94-1-2-2, IR 73007-131-1-2-2, 1R 70215-
70-CPA3-4-1-3, IR 80411-B-28-4, IR 79913-B-176-B-4, IR 78937-B-20-B-B-4, BR 4363-3-
8-1-2-4(BR-32), Basundhara, Tarahara-1, Hardinath-2, Sura vi and Mahima were free from
blast in both the years. The number of moderately resistant (3 -4), moderately susceptible (5 -6)
and highly susceptible (7-9) genotypes was 43, 9 and 2 in 2011 and 54, 3 and 1 in 2012,
respectively. Disease severity increased in second and third scorings in all entries except the
resistant ones. IR09Fll0, NR 1887-8-1-1-2 and Kanchhi masuli were highly susceptible to
blast with the highest disease score (7) which were completely knocked down in the final
scoring.Link for e-copy: http://elibrary.narc.gov.np/?r=919 E-copies
http://nkcs.org.np/narc/dl/?r=919URL
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Title : Seed treatment evaluation of different fungicides to control blast disease of rice Material Type: printed text Authors: Vaidya, M.L., Editor ; Parajuli, G.P., Author Publication Date: 2010 Pagination: 424-426 p. Keywords: Blast ; rice Abstract: Blast (Pyricularia o1yzae) is the serious problem of local Rice cultivar Jumli Marshi at high altitude region of Nepal and causes
significant losses in yield. Thus, in order to detennine the chemical control against Blast disease grown at the irrigated condition, a
field experiment was conducted at the Agricultural Research Station, Jumla, Nepal in 2004-2005 summer season. This rice variety,
Jumli marshi local, was treated with different doses of chemicals like Beam, Bavistin and Hinosan. The most effective chemical on
seed treatment was found to be Beam @2gm/kg of seed before sowing, which yielded 2842 kglha. whereas Beam @1.5gmlkg of
seed treatment yielded 2224 kglha.Link for e-copy: http://elibrary.narc.gov.np/?r=1047 Seed treatment evaluation of different fungicides to control blast disease of rice [printed text] / Vaidya, M.L., Editor ; Parajuli, G.P., Author . - 2010 . - 424-426 p.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Keywords: Blast ; rice Abstract: Blast (Pyricularia o1yzae) is the serious problem of local Rice cultivar Jumli Marshi at high altitude region of Nepal and causes
significant losses in yield. Thus, in order to detennine the chemical control against Blast disease grown at the irrigated condition, a
field experiment was conducted at the Agricultural Research Station, Jumla, Nepal in 2004-2005 summer season. This rice variety,
Jumli marshi local, was treated with different doses of chemicals like Beam, Bavistin and Hinosan. The most effective chemical on
seed treatment was found to be Beam @2gm/kg of seed before sowing, which yielded 2842 kglha. whereas Beam @1.5gmlkg of
seed treatment yielded 2224 kglha.Link for e-copy: http://elibrary.narc.gov.np/?r=1047 E-copies
http://nkcs.org.np/narc/dl/?r=1047URLEFFICACY OF BEAM 75 WP IN CONTROLLING LEAF BLAST DISEASE AT THE SEEDLING STAGE OF RICE / B. Chaudhary in Nepal Agriculture Research Journal, Vol.2, No.1 (1998)
Title : EFFICACY OF BEAM 75 WP IN CONTROLLING LEAF BLAST DISEASE AT THE SEEDLING STAGE OF RICE Material Type: printed text Authors: B. Chaudhary, Author ; Sah, D.N., Author Publication Date: 1998 Article on page: 42-47 p. Languages : English (eng) Keywords: Beam 75 WP, Disease severity, Leaf blast reducing efficiency, Pyricularia grisea,Tricyclazole Link for e-copy: http://elibrary.narc.gov.np/?r=1168
in Nepal Agriculture Research Journal > Vol.2, No.1 (1998) . - 42-47 p.[article] EFFICACY OF BEAM 75 WP IN CONTROLLING LEAF BLAST DISEASE AT THE SEEDLING STAGE OF RICE [printed text] / B. Chaudhary, Author ; Sah, D.N., Author . - 1998 . - 42-47 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol.2, No.1 (1998) . - 42-47 p.
Keywords: Beam 75 WP, Disease severity, Leaf blast reducing efficiency, Pyricularia grisea,Tricyclazole Link for e-copy: http://elibrary.narc.gov.np/?r=1168
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Title : Evaluation of rice genotypes for resistance to blast disease Material Type: printed text Authors: B. Chaudhary ; M Yadav, Author ; Yadaw, R.B., Author ; Chaudhary, D.K., Author ; PCP Chaurasiya, Author ; Chaudhary, R.N., Author ; B.N Mahato, Author ; Sah, D.N., Author Pagination: 327-335 p. Languages : English (eng) Keywords: Blast fungus ; rice genotypes sources of resistance pathogen variability Abstract: exotic were evaluated for resistance to blast over four locations during 2002 and 2003,
respectively. Disease severity varied over locations and years. Within locations, genotypes
showed variation in disease severity. During 2002, 186 entries were resistant (R), 40
moderately resistant (MR.), 14 moderately susceptible {MS), 32 susceptible (S) to disease at
Hardinath. At Lumle, 69 entries scored R, 75 MR, 66 MS and 62 S to the disease. At Rampur,
174 entries rated R, 19 MR and 3 MS to the disease while 78 failed to germinate. At
Tarahara, 236 entries showed R, 6 MR and 30 entries did nopt germinate. During 2003, 148
entries showed R, 16 MR, 4 MS and 20 S to the disease at Hardinath. At Tarahara, all the
tested entries were resistant to disease, indicating escape from disease during the growing
season. At Khumaltar, 169 entries scored R, 59 MR, 29 MS and 8 S to disease. At Nawalpur,
217 entries rated R, 21 MR, 9 MS and 11 S to the disease. However, 39 and 123 lines were
resistant to the pathogen over locations during 2002 and 2003 tests, respectively. This
indicated that the genotypes varied for disease reaction over locations suggesting the
prevalence of variable virulence in the pathogen population. In neck blast screening, 11
genotypes out of 274 entries showed R to MR to the disease over Hardinath and Rampur
during 2002 test. In 2003, 132 genotypes were R to MR to the disease at Hardinath. Blast
nursery also included 166 entries received from International Rice Research Institute in
2002. Of them, 60 entries were found resistant to the disease over Hardinath and Rampur.Link for e-copy: http://elibrary.narc.gov.np/?r=1119 Evaluation of rice genotypes for resistance to blast disease [printed text] / B. Chaudhary ; M Yadav, Author ; Yadaw, R.B., Author ; Chaudhary, D.K., Author ; PCP Chaurasiya, Author ; Chaudhary, R.N., Author ; B.N Mahato, Author ; Sah, D.N., Author . - [s.d.] . - 327-335 p.
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Languages : English (eng)
Keywords: Blast fungus ; rice genotypes sources of resistance pathogen variability Abstract: exotic were evaluated for resistance to blast over four locations during 2002 and 2003,
respectively. Disease severity varied over locations and years. Within locations, genotypes
showed variation in disease severity. During 2002, 186 entries were resistant (R), 40
moderately resistant (MR.), 14 moderately susceptible {MS), 32 susceptible (S) to disease at
Hardinath. At Lumle, 69 entries scored R, 75 MR, 66 MS and 62 S to the disease. At Rampur,
174 entries rated R, 19 MR and 3 MS to the disease while 78 failed to germinate. At
Tarahara, 236 entries showed R, 6 MR and 30 entries did nopt germinate. During 2003, 148
entries showed R, 16 MR, 4 MS and 20 S to the disease at Hardinath. At Tarahara, all the
tested entries were resistant to disease, indicating escape from disease during the growing
season. At Khumaltar, 169 entries scored R, 59 MR, 29 MS and 8 S to disease. At Nawalpur,
217 entries rated R, 21 MR, 9 MS and 11 S to the disease. However, 39 and 123 lines were
resistant to the pathogen over locations during 2002 and 2003 tests, respectively. This
indicated that the genotypes varied for disease reaction over locations suggesting the
prevalence of variable virulence in the pathogen population. In neck blast screening, 11
genotypes out of 274 entries showed R to MR to the disease over Hardinath and Rampur
during 2002 test. In 2003, 132 genotypes were R to MR to the disease at Hardinath. Blast
nursery also included 166 entries received from International Rice Research Institute in
2002. Of them, 60 entries were found resistant to the disease over Hardinath and Rampur.Link for e-copy: http://elibrary.narc.gov.np/?r=1119 E-copies
http://nkcs.org.np/narc/dl/?r=1119URLMolecular Tagging, Allele mining and marker aided breeding for blast resistance in rice / Joshi, Bal K.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Title : Molecular Tagging, Allele mining and marker aided breeding for blast resistance in rice Material Type: printed text Authors: Joshi, Bal K., Editor ; Bimb, Hari P., Author ; Gopal Parajuli, Author ; Bedanand Chaudhary, Author Publication Date: 2010 Pagination: 189-202 p. Keywords: Blast resistance ; DNA marker Gene tagging rice Abstract: Breeding work utilizing both genotypic and phenotypic· markers is the most effective way of achieving target. Molecular markers eg
restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and simi'~~ sequence repeat (SSR) tightly
linked to target gene have been identified in different chromosomes to impose the genetic selection ie marker assisted selection
(MAS). This paper summarize the progress and achievement made in breeding for blast resistance based on DNA markers which
help in planning blast resistance gene tagging in Nepalese rice genotypes and developing blast resistant inbred line or near isogenic
line (NIL). Blast fungus (Pyricularia oryzae Cav.) can infect plants at any growth stage from seedling to maturity and at any part eg
leaf, node, internode, neck and seed. Qualitative resistance gene may occasionally be broken down due to numerous races of blast
fungus both physiological and geographical races available in Nepal. Quantitative gene resistance and gene pyramiding are the best
alternative for creating durable resistance system. At least 40 genes conferring resistance to blast isolates with multiple alleles have
been described. Both dominant and recessive resistance alleles have been found in many rice landraces. Morphological and isozymic
markers are limited in number. Therefore, highly polymorphic and easily detectable SSR markers are being used in breeding for
blast resistance. Bulked segregant analysis (BSA) is the simple method for tagging resistance ge~e by SSR markers. Quantitative
trait loci (QTLs) have also been mapped and· most of them are linked to qualitative genes. SSR markers linked to the gene are being
used to select plants possessing the desired trait and markers throughout the genome are being used to select plants that are
genetically similar to recurrent parent. Using SSR markers it may be possible to select blast resistance genotypes at any stage of crop
development from any small part of crop, to conduct many round of selection, to select without inoculums, without scoring, and
without testing in hot spot or artificial inoculation. Molecular based blast resistance breeding work is initiated focusing on resistance
gene tagging in Nepalese rice Iandraces and transferring resistance genes in cvs Jumli Marshi, K.humal-4 and Mansuli.Link for e-copy: http://elibrary.narc.gov.np/?r=1016 Molecular Tagging, Allele mining and marker aided breeding for blast resistance in rice [printed text] / Joshi, Bal K., Editor ; Bimb, Hari P., Author ; Gopal Parajuli, Author ; Bedanand Chaudhary, Author . - 2010 . - 189-202 p.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Keywords: Blast resistance ; DNA marker Gene tagging rice Abstract: Breeding work utilizing both genotypic and phenotypic· markers is the most effective way of achieving target. Molecular markers eg
restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and simi'~~ sequence repeat (SSR) tightly
linked to target gene have been identified in different chromosomes to impose the genetic selection ie marker assisted selection
(MAS). This paper summarize the progress and achievement made in breeding for blast resistance based on DNA markers which
help in planning blast resistance gene tagging in Nepalese rice genotypes and developing blast resistant inbred line or near isogenic
line (NIL). Blast fungus (Pyricularia oryzae Cav.) can infect plants at any growth stage from seedling to maturity and at any part eg
leaf, node, internode, neck and seed. Qualitative resistance gene may occasionally be broken down due to numerous races of blast
fungus both physiological and geographical races available in Nepal. Quantitative gene resistance and gene pyramiding are the best
alternative for creating durable resistance system. At least 40 genes conferring resistance to blast isolates with multiple alleles have
been described. Both dominant and recessive resistance alleles have been found in many rice landraces. Morphological and isozymic
markers are limited in number. Therefore, highly polymorphic and easily detectable SSR markers are being used in breeding for
blast resistance. Bulked segregant analysis (BSA) is the simple method for tagging resistance ge~e by SSR markers. Quantitative
trait loci (QTLs) have also been mapped and· most of them are linked to qualitative genes. SSR markers linked to the gene are being
used to select plants possessing the desired trait and markers throughout the genome are being used to select plants that are
genetically similar to recurrent parent. Using SSR markers it may be possible to select blast resistance genotypes at any stage of crop
development from any small part of crop, to conduct many round of selection, to select without inoculums, without scoring, and
without testing in hot spot or artificial inoculation. Molecular based blast resistance breeding work is initiated focusing on resistance
gene tagging in Nepalese rice Iandraces and transferring resistance genes in cvs Jumli Marshi, K.humal-4 and Mansuli.Link for e-copy: http://elibrary.narc.gov.np/?r=1016 E-copies
http://nkcs.org.np/narc/dl/?r=1016URL
