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14 result(s) search for keyword(s) 'Blast'
Molecular Tagging, Allele mining and marker aided breeding for blast resistance in rice / Joshi, Bal K.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Title : Molecular Tagging, Allele mining and marker aided breeding for blast resistance in rice Material Type: printed text Authors: Joshi, Bal K., Editor ; Bimb, Hari P., Author ; Gopal Parajuli, Author ; Bedanand Chaudhary, Author Publication Date: 2010 Pagination: 189-202 p. Keywords: Blast resistance ; DNA marker Gene tagging rice Abstract: Breeding work utilizing both genotypic and phenotypic· markers is the most effective way of achieving target. Molecular markers eg
restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and simi'~~ sequence repeat (SSR) tightly
linked to target gene have been identified in different chromosomes to impose the genetic selection ie marker assisted selection
(MAS). This paper summarize the progress and achievement made in breeding for blast resistance based on DNA markers which
help in planning blast resistance gene tagging in Nepalese rice genotypes and developing blast resistant inbred line or near isogenic
line (NIL). Blast fungus (Pyricularia oryzae Cav.) can infect plants at any growth stage from seedling to maturity and at any part eg
leaf, node, internode, neck and seed. Qualitative resistance gene may occasionally be broken down due to numerous races of blast
fungus both physiological and geographical races available in Nepal. Quantitative gene resistance and gene pyramiding are the best
alternative for creating durable resistance system. At least 40 genes conferring resistance to blast isolates with multiple alleles have
been described. Both dominant and recessive resistance alleles have been found in many rice landraces. Morphological and isozymic
markers are limited in number. Therefore, highly polymorphic and easily detectable SSR markers are being used in breeding for
blast resistance. Bulked segregant analysis (BSA) is the simple method for tagging resistance ge~e by SSR markers. Quantitative
trait loci (QTLs) have also been mapped and· most of them are linked to qualitative genes. SSR markers linked to the gene are being
used to select plants possessing the desired trait and markers throughout the genome are being used to select plants that are
genetically similar to recurrent parent. Using SSR markers it may be possible to select blast resistance genotypes at any stage of crop
development from any small part of crop, to conduct many round of selection, to select without inoculums, without scoring, and
without testing in hot spot or artificial inoculation. Molecular based blast resistance breeding work is initiated focusing on resistance
gene tagging in Nepalese rice Iandraces and transferring resistance genes in cvs Jumli Marshi, K.humal-4 and Mansuli.Link for e-copy: http://elibrary.narc.gov.np/?r=1016 Molecular Tagging, Allele mining and marker aided breeding for blast resistance in rice [printed text] / Joshi, Bal K., Editor ; Bimb, Hari P., Author ; Gopal Parajuli, Author ; Bedanand Chaudhary, Author . - 2010 . - 189-202 p.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Keywords: Blast resistance ; DNA marker Gene tagging rice Abstract: Breeding work utilizing both genotypic and phenotypic· markers is the most effective way of achieving target. Molecular markers eg
restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and simi'~~ sequence repeat (SSR) tightly
linked to target gene have been identified in different chromosomes to impose the genetic selection ie marker assisted selection
(MAS). This paper summarize the progress and achievement made in breeding for blast resistance based on DNA markers which
help in planning blast resistance gene tagging in Nepalese rice genotypes and developing blast resistant inbred line or near isogenic
line (NIL). Blast fungus (Pyricularia oryzae Cav.) can infect plants at any growth stage from seedling to maturity and at any part eg
leaf, node, internode, neck and seed. Qualitative resistance gene may occasionally be broken down due to numerous races of blast
fungus both physiological and geographical races available in Nepal. Quantitative gene resistance and gene pyramiding are the best
alternative for creating durable resistance system. At least 40 genes conferring resistance to blast isolates with multiple alleles have
been described. Both dominant and recessive resistance alleles have been found in many rice landraces. Morphological and isozymic
markers are limited in number. Therefore, highly polymorphic and easily detectable SSR markers are being used in breeding for
blast resistance. Bulked segregant analysis (BSA) is the simple method for tagging resistance ge~e by SSR markers. Quantitative
trait loci (QTLs) have also been mapped and· most of them are linked to qualitative genes. SSR markers linked to the gene are being
used to select plants possessing the desired trait and markers throughout the genome are being used to select plants that are
genetically similar to recurrent parent. Using SSR markers it may be possible to select blast resistance genotypes at any stage of crop
development from any small part of crop, to conduct many round of selection, to select without inoculums, without scoring, and
without testing in hot spot or artificial inoculation. Molecular based blast resistance breeding work is initiated focusing on resistance
gene tagging in Nepalese rice Iandraces and transferring resistance genes in cvs Jumli Marshi, K.humal-4 and Mansuli.Link for e-copy: http://elibrary.narc.gov.np/?r=1016 E-copies
http://nkcs.org.np/narc/dl/?r=1016URL
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Title : Seed treatment evaluation of different fungicides to control blast disease of rice Material Type: printed text Authors: Vaidya, M.L., Editor ; Parajuli, G.P., Author Publication Date: 2010 Pagination: 424-426 p. Keywords: Blast ; rice Abstract: Blast (Pyricularia o1yzae) is the serious problem of local Rice cultivar Jumli Marshi at high altitude region of Nepal and causes
significant losses in yield. Thus, in order to detennine the chemical control against Blast disease grown at the irrigated condition, a
field experiment was conducted at the Agricultural Research Station, Jumla, Nepal in 2004-2005 summer season. This rice variety,
Jumli marshi local, was treated with different doses of chemicals like Beam, Bavistin and Hinosan. The most effective chemical on
seed treatment was found to be Beam @2gm/kg of seed before sowing, which yielded 2842 kglha. whereas Beam @1.5gmlkg of
seed treatment yielded 2224 kglha.Link for e-copy: http://elibrary.narc.gov.np/?r=1047 Seed treatment evaluation of different fungicides to control blast disease of rice [printed text] / Vaidya, M.L., Editor ; Parajuli, G.P., Author . - 2010 . - 424-426 p.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Keywords: Blast ; rice Abstract: Blast (Pyricularia o1yzae) is the serious problem of local Rice cultivar Jumli Marshi at high altitude region of Nepal and causes
significant losses in yield. Thus, in order to detennine the chemical control against Blast disease grown at the irrigated condition, a
field experiment was conducted at the Agricultural Research Station, Jumla, Nepal in 2004-2005 summer season. This rice variety,
Jumli marshi local, was treated with different doses of chemicals like Beam, Bavistin and Hinosan. The most effective chemical on
seed treatment was found to be Beam @2gm/kg of seed before sowing, which yielded 2842 kglha. whereas Beam @1.5gmlkg of
seed treatment yielded 2224 kglha.Link for e-copy: http://elibrary.narc.gov.np/?r=1047 E-copies
http://nkcs.org.np/narc/dl/?r=1047URL ResistanceAnd Susceptible Interaction Of Different Rice Lines Against Leaf And Neck Blast(Pyricularia Sacc.) / Puri, K.D
Title : ResistanceAnd Susceptible Interaction Of Different Rice Lines Against Leaf And Neck Blast(Pyricularia Sacc.) Material Type: printed text Authors: Puri, K.D, Author Publisher: Chitwan : Tribhuvan University Publication Date: 2006 Pagination: 109 p. Languages : English (eng) Keywords: rice blast disease;disease cycle;crop nutrition and infection;epidemics of the Link for e-copy: http://elibrary.narc.gov.np/?r=3217 ResistanceAnd Susceptible Interaction Of Different Rice Lines Against Leaf And Neck Blast(Pyricularia Sacc.) [printed text] / Puri, K.D, Author . - Chitwan : Tribhuvan University, 2006 . - 109 p.
Languages : English (eng)
Keywords: rice blast disease;disease cycle;crop nutrition and infection;epidemics of the Link for e-copy: http://elibrary.narc.gov.np/?r=3217 Copies (1)
Barcode Call number Media type Location Section Status 5452 PUR Document NARC Library Documents Available Resistance in Rice Breeding Lines to the Blast Fungus in Nepal / Bedananda Chaudhary in Nepal Agriculture Research Journal, Vol. 6 (2005)
[article]
Title : Resistance in Rice Breeding Lines to the Blast Fungus in Nepal Material Type: printed text Authors: Bedananda Chaudhary, Author ; Sundar M. Shrestha, Author ; Sharma, R.C., Author Publication Date: 2005 Article on page: 49-56 p Languages : English (eng) Keywords: Blast resistance, field condition, greenhouse assay, Pyricularia grisea, rice lines Abstract: A total of 36 rice breeding lines including checks were evaluated for resistance to blast at
Rampur during 2000-2001. The experiments were conducted under both field condition and
greenhouse inoculated condition. Qualitative resistance in rice to blast was assessed based on
lesion type, whereas quantitative resistance was assessed based on area under disease progress
curve (AUDPC) in the upland field condition. The number of sporulating lesions and the number
of leaves with at least one sporulating lesion per plant were considered as measures for
evaluation of quantitative resistance in the greenhouse assay. The lesion type, neck blast
percentage and AUDPC data suggest that most of the rice lines possess higher level of resistance
to leaf and neck blast. The rice lines varied for the number of sporulating lesions and the number
of leaves with sporulating lesion per plant. Some lines were incompatible to virulent blast
isolates, showing major resistance genes. NR 1558, NR 601-1-1-9, BW306-2 and CN 836-3-10
were promising lines for quantitative resistance to both leaf and neck blast. Radha 12, Sabitri,
Janaki possess higher level of quantitative resistance to blast, hence these could be promoted for
cultivation in blast-prone environments. These genotypes could also be utilized as donor parents
for breeding durable blast resistant varieties. The most virulent blast isolate could be used for
evaluation of both qualitative and quantitative resistance to blast in early generation in the
greenhouse so that workload could be cut down in future works.Link for e-copy: http://elibrary.narc.gov.np/?r=61
in Nepal Agriculture Research Journal > Vol. 6 (2005) . - 49-56 p[article] Resistance in Rice Breeding Lines to the Blast Fungus in Nepal [printed text] / Bedananda Chaudhary, Author ; Sundar M. Shrestha, Author ; Sharma, R.C., Author . - 2005 . - 49-56 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 6 (2005) . - 49-56 p
Keywords: Blast resistance, field condition, greenhouse assay, Pyricularia grisea, rice lines Abstract: A total of 36 rice breeding lines including checks were evaluated for resistance to blast at
Rampur during 2000-2001. The experiments were conducted under both field condition and
greenhouse inoculated condition. Qualitative resistance in rice to blast was assessed based on
lesion type, whereas quantitative resistance was assessed based on area under disease progress
curve (AUDPC) in the upland field condition. The number of sporulating lesions and the number
of leaves with at least one sporulating lesion per plant were considered as measures for
evaluation of quantitative resistance in the greenhouse assay. The lesion type, neck blast
percentage and AUDPC data suggest that most of the rice lines possess higher level of resistance
to leaf and neck blast. The rice lines varied for the number of sporulating lesions and the number
of leaves with sporulating lesion per plant. Some lines were incompatible to virulent blast
isolates, showing major resistance genes. NR 1558, NR 601-1-1-9, BW306-2 and CN 836-3-10
were promising lines for quantitative resistance to both leaf and neck blast. Radha 12, Sabitri,
Janaki possess higher level of quantitative resistance to blast, hence these could be promoted for
cultivation in blast-prone environments. These genotypes could also be utilized as donor parents
for breeding durable blast resistant varieties. The most virulent blast isolate could be used for
evaluation of both qualitative and quantitative resistance to blast in early generation in the
greenhouse so that workload could be cut down in future works.Link for e-copy: http://elibrary.narc.gov.np/?r=61
in Proceedings of Progress Review Workshop of Fingermillet under IFAD-NUS Project - Nepal Component, January 28, 2005 / NARC
Title : A disease survey report on fingermillet in eastern and western hills of Nepal. Material Type: printed text Authors: Prasad, R.C. ; Khanal B, Author Publication Date: 2005 Pagination: 30-31 p. Languages : English (eng) Keywords: Survey, diseases, fingermillet, pyricularia grasea, blast Abstract: A survey programme was conducted on fingermillet in 2004 to hilly regions of the eastern and . western part of the country to know the situation of millet diseases and to study about them. Some millet growing of Dolakha, Sidhupalchowk, Syanja, Tanahu, Kaski & Nuwakot district were visited and appearance diseases were recorded at randomly selecting Ten plants in each field. Blast (Pyricularia grisea), Cercospora Leaf spot (Cercospora eleusine) and Helminthosporium leaf spot (Helminthosporium spp.) were observed among them Cercospora leaf spot disease was found only at higher altitude. Low severities of major diseases (Blast and CLS) were observed in all districts. Finger blast was observed ·up to resistant reaction in all the surveyed districts. The agronomically superior cultivars should be selected to maintain the genetic purity and seed multiplication programme for next season to distribute the farmers of that area.
Link for e-copy: http://elibrary.narc.gov.np/?r=519 A disease survey report on fingermillet in eastern and western hills of Nepal. [printed text] / Prasad, R.C. ; Khanal B, Author . - 2005 . - 30-31 p.
in Proceedings of Progress Review Workshop of Fingermillet under IFAD-NUS Project - Nepal Component, January 28, 2005 / NARC
Languages : English (eng)
Keywords: Survey, diseases, fingermillet, pyricularia grasea, blast Abstract: A survey programme was conducted on fingermillet in 2004 to hilly regions of the eastern and . western part of the country to know the situation of millet diseases and to study about them. Some millet growing of Dolakha, Sidhupalchowk, Syanja, Tanahu, Kaski & Nuwakot district were visited and appearance diseases were recorded at randomly selecting Ten plants in each field. Blast (Pyricularia grisea), Cercospora Leaf spot (Cercospora eleusine) and Helminthosporium leaf spot (Helminthosporium spp.) were observed among them Cercospora leaf spot disease was found only at higher altitude. Low severities of major diseases (Blast and CLS) were observed in all districts. Finger blast was observed ·up to resistant reaction in all the surveyed districts. The agronomically superior cultivars should be selected to maintain the genetic purity and seed multiplication programme for next season to distribute the farmers of that area.
Link for e-copy: http://elibrary.narc.gov.np/?r=519 E-copies
http://nkcs.org.np/narc/dl/?r=519URL PermalinkPermalinkPermalinkResource mapping of the fingermillet growing project sites of hill crops research program, Kabre, Dolakha and Li-Bird, Pokhara, Kaski / Rai, S.K.PermalinkPermalinkEFFICACY OF BEAM 75 WP IN CONTROLLING LEAF BLAST DISEASE AT THE SEEDLING STAGE OF RICE / B. Chaudhary in Nepal Agriculture Research Journal, Vol.2, No.1 (1998)PermalinkPermalinkPermalinkPermalink