Meristem Culture of Mandarin Orange (Citrus reticulata) for Elimination of Citrus Tristeza Virus / Radha Panthi in Nepalese Horticulture, v. 8/9 ([07/25/2012])

Public ISBD [article]  inNepalese Horticulture > v. 8/9 [07/25/2012] . - 63-68 p. Title : Meristem Culture of Mandarin Orange (Citrus reticulata) for Elimination of Citrus Tristeza Virus Material Type: printed text Authors: Radha Panthi, Author ; Ranjit, Mukunda, Author Publication Date: 2012 Article on page: 63-68 p. Languages : English (eng) Keywords: CTV,  DAS-ELISA,  Meristem  Tissue  Culture,  In  vitro Abstract: Mandarin trees grafted on trifoliate orange showing symptoms of citrus tristeza virus (CTV) such as yellowing of leaves, interveinal chlorosis and stem pitting were subjected to virus testing by double antibody sandwich enzyme-linked immuno sorbent assay (DAS-ELISA). Results with optical density (OD405) of more than 0.5 have confirmed the presence of CTV. Culture of apical meristems with few leaf primordia ranging from 0.2 to 0.5 mm in diameter has given rise to virus-free plantlets (OD405 of 0.16) after eight weeks. This technique of meristem culture may be useful to eliminate viruses from elite mandarin plants. [article] Meristem Culture of Mandarin Orange (Citrus reticulata) for Elimination of Citrus Tristeza Virus [printed text] / Radha Panthi, Author ; Ranjit, Mukunda, Author . - 2012 . - 63-68 p. Languages : English (eng) in Nepalese Horticulture > v. 8/9 [07/25/2012] . - 63-68 p. Keywords: CTV,  DAS-ELISA,  Meristem  Tissue  Culture,  In  vitro Abstract: Mandarin trees grafted on trifoliate orange showing symptoms of citrus tristeza virus (CTV) such as yellowing of leaves, interveinal chlorosis and stem pitting were subjected to virus testing by double antibody sandwich enzyme-linked immuno sorbent assay (DAS-ELISA). Results with optical density (OD405) of more than 0.5 have confirmed the presence of CTV. Culture of apical meristems with few leaf primordia ranging from 0.2 to 0.5 mm in diameter has given rise to virus-free plantlets (OD405 of 0.16) after eight weeks. This technique of meristem culture may be useful to eliminate viruses from elite mandarin plants.