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in
| Title : |
Genetic Diversity in Nepalese Large Cardamom Gene Pool Based on Rapd Markers |
| Material Type: |
printed text |
| Authors: |
Raju Chaudhary, Author ; Niroula, Raj Kumar, Author ; Shrawan Kumar Sah, Author ; Pradip Kumar Yadav, Author ; Bal Krishna Joshi, Author ; Ojha, Bishnu Raj, Author ; Surya Kant Ghimire, Author |
| Pagination: |
25-30 p. |
| Languages : |
English (eng) |
| Keywords: |
Large cardamom (Amomum subulatum), Churumpha, RAPD, decamcr, genetic diversity |
| Abstract: |
Large Cardamom (Amomum subulatum Roxb.) is a major cash generating spice crop of Nepal, mainly
confined to the eastern hills of Nepal. Fourteen large cardamom samples collected from Taplejung,
Panchthar and Jlam Districts of Nepal during 2012 were used to evaluate genetic diversity using
RAPD markers. Total of fifty one decamer primers (Operon series) were initially used for the
experiment but only eighteen primers were selected based on nature of polym01phic and
reproducibility for the analysis. Out of 133 loci generated 97.74 % were found to be polymorphic with
number of alleles 1.977±0.013 and number of effective alleles1.409±0.024. Considering a single
population Nei 's genetic diversity over all loci generated was found to be 0.263±0. OJ 2 with Shannon's
information· index 0.416. On UPGMA clustering, two main clusters were obse1,ed that clearly
separate wild cultivars (Churumpha) from commercially grown cultivars. The RAPD markers showed
sufficient polymorphism for genotype discrimination and experimental reproducibility. Incorporation
of molecular approaches over conventional method of genetic diversity assessment is. very much
effective in pinpointing the potential genetic diversity for cardamom improvement. |
| Link for e-copy: |
http://elibrary.narc.gov.np/?r=1543 |
in
Genetic Diversity in Nepalese Large Cardamom Gene Pool Based on Rapd Markers [printed text] / Raju Chaudhary, Author ; Niroula, Raj Kumar, Author ; Shrawan Kumar Sah, Author ; Pradip Kumar Yadav, Author ; Bal Krishna Joshi, Author ; Ojha, Bishnu Raj, Author ; Surya Kant Ghimire, Author . - [s.d.] . - 25-30 p. Languages : English ( eng) | Keywords: |
Large cardamom (Amomum subulatum), Churumpha, RAPD, decamcr, genetic diversity |
| Abstract: |
Large Cardamom (Amomum subulatum Roxb.) is a major cash generating spice crop of Nepal, mainly
confined to the eastern hills of Nepal. Fourteen large cardamom samples collected from Taplejung,
Panchthar and Jlam Districts of Nepal during 2012 were used to evaluate genetic diversity using
RAPD markers. Total of fifty one decamer primers (Operon series) were initially used for the
experiment but only eighteen primers were selected based on nature of polym01phic and
reproducibility for the analysis. Out of 133 loci generated 97.74 % were found to be polymorphic with
number of alleles 1.977±0.013 and number of effective alleles1.409±0.024. Considering a single
population Nei 's genetic diversity over all loci generated was found to be 0.263±0. OJ 2 with Shannon's
information· index 0.416. On UPGMA clustering, two main clusters were obse1,ed that clearly
separate wild cultivars (Churumpha) from commercially grown cultivars. The RAPD markers showed
sufficient polymorphism for genotype discrimination and experimental reproducibility. Incorporation
of molecular approaches over conventional method of genetic diversity assessment is. very much
effective in pinpointing the potential genetic diversity for cardamom improvement. |
| Link for e-copy: |
http://elibrary.narc.gov.np/?r=1543 |
|
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