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Author Hamal, Shreya S. |
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Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. / Madan Raj Bhatta
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author ; Ojha, Bishnu R., Author ; Surya K. Ghimire, Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 23-30 p. Languages : English (eng) Keywords: 'elongation', 'hypoxia', 'SublA', 'flooding', 'SNORKEL' Abstract: Flooding is a notorious environmental stresses that limits the rice production in Asia significantly. Rice has a unique capacity to withstand the period of flooding environment through the activation of coordinated regulatory efforts at morphological, anatomical, biochemical and molecular level. Three hundred and thirteen diverse rice collections including selected landraces from Nepal were completely submerged for 14 days for this study. Rice accessions had diverse response to submergence with survival ity ranging from 0 to 100 % and differential elongation growth response. Presence of Sub 1 A-1 and SNORKEL genes were then monitored in the tolerant landraces. Four landraces; Bhaatsaar, Kariyaparewa pakha, Sauthari and Karangi and 2 landraces; NPGR 3501 and NPGR 3503 showed better response than FRl 3A and Kalamon 113 respectively. However, Kariyaparewa pakha and Sauthari were found lacking the presence of Sub 1 A-1 allele. In addition, eight landraces with Sub I A- I allele were found intolerant to submergence. However, the SPAD reading showed that FR 13A had the highest chlorophyll retention capacity. Thus, the detail morpho-physiological and molecular analysis showed that both the tolerant and intolerant landraces possessed the SublA-1 allele and they showed both escape and quiescence strategy to cope the flooding. Therefore, SublA-1 allele is not the only ones responsible for tolerance but there is the participation of other stress responsive factors.
Link for e-copy: http://elibrary.narc.gov.np/?r=1489 Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author ; Ojha, Bishnu R., Author ; Surya K. Ghimire, Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 23-30 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'elongation', 'hypoxia', 'SublA', 'flooding', 'SNORKEL' Abstract: Flooding is a notorious environmental stresses that limits the rice production in Asia significantly. Rice has a unique capacity to withstand the period of flooding environment through the activation of coordinated regulatory efforts at morphological, anatomical, biochemical and molecular level. Three hundred and thirteen diverse rice collections including selected landraces from Nepal were completely submerged for 14 days for this study. Rice accessions had diverse response to submergence with survival ity ranging from 0 to 100 % and differential elongation growth response. Presence of Sub 1 A-1 and SNORKEL genes were then monitored in the tolerant landraces. Four landraces; Bhaatsaar, Kariyaparewa pakha, Sauthari and Karangi and 2 landraces; NPGR 3501 and NPGR 3503 showed better response than FRl 3A and Kalamon 113 respectively. However, Kariyaparewa pakha and Sauthari were found lacking the presence of Sub 1 A-1 allele. In addition, eight landraces with Sub I A- I allele were found intolerant to submergence. However, the SPAD reading showed that FR 13A had the highest chlorophyll retention capacity. Thus, the detail morpho-physiological and molecular analysis showed that both the tolerant and intolerant landraces possessed the SublA-1 allele and they showed both escape and quiescence strategy to cope the flooding. Therefore, SublA-1 allele is not the only ones responsible for tolerance but there is the participation of other stress responsive factors.
Link for e-copy: http://elibrary.narc.gov.np/?r=1489 E-copies
http://nkcs.org.np/narc/dl/?r=1489URL Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. / Madan Raj Bhatta
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 18-22 p. Languages : English (eng) Keywords: 'durable', 'resistant', 'susceptible', 'pathotype', 'pyramiding' Abstract: Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on the availability and use of resistant gene donors. Ninety six Nepalese rice accessions were screened using eight SSR markers and one STS marker for presence and absence of BLB resistance gene. We detected BLB resistance gene Xa-10 on two accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-10 on five accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that different BLB pathotypes are active in Nepalese rice field and need of gene pyramiding for durable resistance.
Link for e-copy: http://elibrary.narc.gov.np/?r=1487 Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 18-22 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'durable', 'resistant', 'susceptible', 'pathotype', 'pyramiding' Abstract: Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on the availability and use of resistant gene donors. Ninety six Nepalese rice accessions were screened using eight SSR markers and one STS marker for presence and absence of BLB resistance gene. We detected BLB resistance gene Xa-10 on two accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-10 on five accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that different BLB pathotypes are active in Nepalese rice field and need of gene pyramiding for durable resistance.
Link for e-copy: http://elibrary.narc.gov.np/?r=1487 E-copies
http://nkcs.org.np/narc/dl/?r=1487URL