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Author Niroula, Raj.K |
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Refine your searchIdentification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. / Madan Raj Bhatta
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author ; Ojha, Bishnu R., Author ; Surya K. Ghimire, Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 23-30 p. Languages : English (eng) Keywords: 'elongation', 'hypoxia', 'SublA', 'flooding', 'SNORKEL' Abstract: Flooding is a notorious environmental stresses that limits the rice production in Asia significantly. Rice has a unique capacity to withstand the period of flooding environment through the activation of coordinated regulatory efforts at morphological, anatomical, biochemical and molecular level. Three hundred and thirteen diverse rice collections including selected landraces from Nepal were completely submerged for 14 days for this study. Rice accessions had diverse response to submergence with survival ity ranging from 0 to 100 % and differential elongation growth response. Presence of Sub 1 A-1 and SNORKEL genes were then monitored in the tolerant landraces. Four landraces; Bhaatsaar, Kariyaparewa pakha, Sauthari and Karangi and 2 landraces; NPGR 3501 and NPGR 3503 showed better response than FRl 3A and Kalamon 113 respectively. However, Kariyaparewa pakha and Sauthari were found lacking the presence of Sub 1 A-1 allele. In addition, eight landraces with Sub I A- I allele were found intolerant to submergence. However, the SPAD reading showed that FR 13A had the highest chlorophyll retention capacity. Thus, the detail morpho-physiological and molecular analysis showed that both the tolerant and intolerant landraces possessed the SublA-1 allele and they showed both escape and quiescence strategy to cope the flooding. Therefore, SublA-1 allele is not the only ones responsible for tolerance but there is the participation of other stress responsive factors.
Link for e-copy: http://elibrary.narc.gov.np/?r=1489 Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author ; Ojha, Bishnu R., Author ; Surya K. Ghimire, Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 23-30 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'elongation', 'hypoxia', 'SublA', 'flooding', 'SNORKEL' Abstract: Flooding is a notorious environmental stresses that limits the rice production in Asia significantly. Rice has a unique capacity to withstand the period of flooding environment through the activation of coordinated regulatory efforts at morphological, anatomical, biochemical and molecular level. Three hundred and thirteen diverse rice collections including selected landraces from Nepal were completely submerged for 14 days for this study. Rice accessions had diverse response to submergence with survival ity ranging from 0 to 100 % and differential elongation growth response. Presence of Sub 1 A-1 and SNORKEL genes were then monitored in the tolerant landraces. Four landraces; Bhaatsaar, Kariyaparewa pakha, Sauthari and Karangi and 2 landraces; NPGR 3501 and NPGR 3503 showed better response than FRl 3A and Kalamon 113 respectively. However, Kariyaparewa pakha and Sauthari were found lacking the presence of Sub 1 A-1 allele. In addition, eight landraces with Sub I A- I allele were found intolerant to submergence. However, the SPAD reading showed that FR 13A had the highest chlorophyll retention capacity. Thus, the detail morpho-physiological and molecular analysis showed that both the tolerant and intolerant landraces possessed the SublA-1 allele and they showed both escape and quiescence strategy to cope the flooding. Therefore, SublA-1 allele is not the only ones responsible for tolerance but there is the participation of other stress responsive factors.
Link for e-copy: http://elibrary.narc.gov.np/?r=1489 E-copies
http://nkcs.org.np/narc/dl/?r=1489URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 18-22 p. Languages : English (eng) Keywords: 'durable', 'resistant', 'susceptible', 'pathotype', 'pyramiding' Abstract: Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on the availability and use of resistant gene donors. Ninety six Nepalese rice accessions were screened using eight SSR markers and one STS marker for presence and absence of BLB resistance gene. We detected BLB resistance gene Xa-10 on two accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-10 on five accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that different BLB pathotypes are active in Nepalese rice field and need of gene pyramiding for durable resistance.
Link for e-copy: http://elibrary.narc.gov.np/?r=1487 Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 18-22 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'durable', 'resistant', 'susceptible', 'pathotype', 'pyramiding' Abstract: Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on the availability and use of resistant gene donors. Ninety six Nepalese rice accessions were screened using eight SSR markers and one STS marker for presence and absence of BLB resistance gene. We detected BLB resistance gene Xa-10 on two accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-10 on five accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that different BLB pathotypes are active in Nepalese rice field and need of gene pyramiding for durable resistance.
Link for e-copy: http://elibrary.narc.gov.np/?r=1487 E-copies
http://nkcs.org.np/narc/dl/?r=1487URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Screening Nepalese rice germplasm for blast resistance characters using molecular markers. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 11-17 p. Languages : English (eng) Keywords: 'Magnaporthe' 'resistant' 'susceptible' 'check' 'gene' Abstract: Blast (caused by Magnaporthe oryzae) is the major yield limiting biotic stress for rice farming in Nepal that is prevalent all over the country. Resistant variety for one location becomes susceptible to another location which shows the need of resistant gene pyramiding for yield stability. Identifying blast resistant genes in Nepalese rice germplasm and its use for varietal development is very important. Ninety two Nepalese rice accessions were screened using 12 SSR markers for presence of different blast resistant genes. IR-64 and Sabitri were used as resistant check while Masuli and Jumli Marshi were used as susceptible check. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t) 1, Pi-z, Pi-a, Pi-k, Pi-yl (t) and Pi-44 were detected on seven, seven, nine, 11, 20 and 28 rice accessions respectively. Eighteen rice accessions showed presence Pi-b and Pi-g(t), 22 rice accessions showed presence of Pi-29 and Pi-11; and two rice accessions showed presence of Pi-ta and Pi20(t). Nepalese rice gene pool doesn't contain resistant gene Pi-d(t)2 and it needs to incorporate from exotic sources. Therefore, many Nepalese rice landraces can be used on marker assisted gene pyramiding program for development of durable blast resistance varieties.
Link for e-copy: http://elibrary.narc.gov.np/?r=1484 Screening Nepalese rice germplasm for blast resistance characters using molecular markers. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 11-17 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'Magnaporthe' 'resistant' 'susceptible' 'check' 'gene' Abstract: Blast (caused by Magnaporthe oryzae) is the major yield limiting biotic stress for rice farming in Nepal that is prevalent all over the country. Resistant variety for one location becomes susceptible to another location which shows the need of resistant gene pyramiding for yield stability. Identifying blast resistant genes in Nepalese rice germplasm and its use for varietal development is very important. Ninety two Nepalese rice accessions were screened using 12 SSR markers for presence of different blast resistant genes. IR-64 and Sabitri were used as resistant check while Masuli and Jumli Marshi were used as susceptible check. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t) 1, Pi-z, Pi-a, Pi-k, Pi-yl (t) and Pi-44 were detected on seven, seven, nine, 11, 20 and 28 rice accessions respectively. Eighteen rice accessions showed presence Pi-b and Pi-g(t), 22 rice accessions showed presence of Pi-29 and Pi-11; and two rice accessions showed presence of Pi-ta and Pi20(t). Nepalese rice gene pool doesn't contain resistant gene Pi-d(t)2 and it needs to incorporate from exotic sources. Therefore, many Nepalese rice landraces can be used on marker assisted gene pyramiding program for development of durable blast resistance varieties.
Link for e-copy: http://elibrary.narc.gov.np/?r=1484 E-copies
http://nkcs.org.np/narc/dl/?r=1484URL
