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Author Pantha, Sumitra |
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Refine your searchEvaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. / Madan Raj Bhatta
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Evaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 6-10 p. Languages : English (eng) Keywords: lesion; severity; incidence; resistant; susceptible; Abstract: Rice diseases are the major problem for rice production and blast is one of them. Development of the resistance varieties instead of using chemical and botanicals to treat the rice blast disease in field is most important in Nepalese context. Therefore, identification of resistance rice accessions from Nepalese rice germplasrn collection is very important for development of blast resistance rice varieties. One hundred and forty four Nepalese rice landraces were screened for their response on blast pathogen in natural condition at Khumaltar, Lalitpur, Nepal. Blast lesion type, disease severity, disease incidence and disease damage were recorded on 25 days old rice seedling. Local rice varieties Jumli Marshi and Masuli were used as susceptible check whereas Sabitri was used as resistant check. One hundred and nine rice landraces showed resistance reaction with blast. Similarly, sixty four rice landraces showed no blast disease symptom during seedling stage. Variation on origin of rice landraces may account for this differential reaction in blast disease development at seedling stage.
Link for e-copy: http://elibrary.narc.gov.np/?r=1482 Evaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 6-10 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: lesion; severity; incidence; resistant; susceptible; Abstract: Rice diseases are the major problem for rice production and blast is one of them. Development of the resistance varieties instead of using chemical and botanicals to treat the rice blast disease in field is most important in Nepalese context. Therefore, identification of resistance rice accessions from Nepalese rice germplasrn collection is very important for development of blast resistance rice varieties. One hundred and forty four Nepalese rice landraces were screened for their response on blast pathogen in natural condition at Khumaltar, Lalitpur, Nepal. Blast lesion type, disease severity, disease incidence and disease damage were recorded on 25 days old rice seedling. Local rice varieties Jumli Marshi and Masuli were used as susceptible check whereas Sabitri was used as resistant check. One hundred and nine rice landraces showed resistance reaction with blast. Similarly, sixty four rice landraces showed no blast disease symptom during seedling stage. Variation on origin of rice landraces may account for this differential reaction in blast disease development at seedling stage.
Link for e-copy: http://elibrary.narc.gov.np/?r=1482 E-copies
http://nkcs.org.np/narc/dl/?r=1482URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author ; Ojha, Bishnu R., Author ; Surya K. Ghimire, Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 23-30 p. Languages : English (eng) Keywords: 'elongation', 'hypoxia', 'SublA', 'flooding', 'SNORKEL' Abstract: Flooding is a notorious environmental stresses that limits the rice production in Asia significantly. Rice has a unique capacity to withstand the period of flooding environment through the activation of coordinated regulatory efforts at morphological, anatomical, biochemical and molecular level. Three hundred and thirteen diverse rice collections including selected landraces from Nepal were completely submerged for 14 days for this study. Rice accessions had diverse response to submergence with survival ity ranging from 0 to 100 % and differential elongation growth response. Presence of Sub 1 A-1 and SNORKEL genes were then monitored in the tolerant landraces. Four landraces; Bhaatsaar, Kariyaparewa pakha, Sauthari and Karangi and 2 landraces; NPGR 3501 and NPGR 3503 showed better response than FRl 3A and Kalamon 113 respectively. However, Kariyaparewa pakha and Sauthari were found lacking the presence of Sub 1 A-1 allele. In addition, eight landraces with Sub I A- I allele were found intolerant to submergence. However, the SPAD reading showed that FR 13A had the highest chlorophyll retention capacity. Thus, the detail morpho-physiological and molecular analysis showed that both the tolerant and intolerant landraces possessed the SublA-1 allele and they showed both escape and quiescence strategy to cope the flooding. Therefore, SublA-1 allele is not the only ones responsible for tolerance but there is the participation of other stress responsive factors.
Link for e-copy: http://elibrary.narc.gov.np/?r=1489 Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author ; Ojha, Bishnu R., Author ; Surya K. Ghimire, Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 23-30 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'elongation', 'hypoxia', 'SublA', 'flooding', 'SNORKEL' Abstract: Flooding is a notorious environmental stresses that limits the rice production in Asia significantly. Rice has a unique capacity to withstand the period of flooding environment through the activation of coordinated regulatory efforts at morphological, anatomical, biochemical and molecular level. Three hundred and thirteen diverse rice collections including selected landraces from Nepal were completely submerged for 14 days for this study. Rice accessions had diverse response to submergence with survival ity ranging from 0 to 100 % and differential elongation growth response. Presence of Sub 1 A-1 and SNORKEL genes were then monitored in the tolerant landraces. Four landraces; Bhaatsaar, Kariyaparewa pakha, Sauthari and Karangi and 2 landraces; NPGR 3501 and NPGR 3503 showed better response than FRl 3A and Kalamon 113 respectively. However, Kariyaparewa pakha and Sauthari were found lacking the presence of Sub 1 A-1 allele. In addition, eight landraces with Sub I A- I allele were found intolerant to submergence. However, the SPAD reading showed that FR 13A had the highest chlorophyll retention capacity. Thus, the detail morpho-physiological and molecular analysis showed that both the tolerant and intolerant landraces possessed the SublA-1 allele and they showed both escape and quiescence strategy to cope the flooding. Therefore, SublA-1 allele is not the only ones responsible for tolerance but there is the participation of other stress responsive factors.
Link for e-copy: http://elibrary.narc.gov.np/?r=1489 E-copies
http://nkcs.org.np/narc/dl/?r=1489URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Molecular screening for rust resistance characteristics in Nepalese barley germplasm. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Sah, Bindeshwar P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 35-43 p. Languages : English (eng) Keywords: 'stripe rust', 'Puccinia', 'resistant', 'durable', 'gene' Abstract: Leaf rust ( caused by Puccinia hordei) and stripe/yellow rust ( caused by P. striformis fsp. hordei and P. striformis fsp. tritici) are the major problem for barley cultivation in Nepal. Durable resistant variety of the barley is highly important for this country. Barley rust resistance is controlled by many major genes and quantitative trait loci (QTLs) and pyramiding these genes into varieties is the most important barley breeding strategies in Nepal. Molecular marker assisted breeding (MAB) is the only option for this. Marker assisted screening is the primary stage to initiate the MAB. One hundred and nine Nepalese barley accessions were screened using six and eleven SSR markers linked to leaf rust and stripe rust resistance genes respectively. Bonus and Local Jau was used as the resistant and susceptible check respectively. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR-P and QTL on 5HS was detected on Nepalese barley accessions using respective SSR markers. Similarly, stripe rust resistance genes QTL on lH, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were detected. Eight Nepalese barley accessions showed presence of three and more leaf rust resistant genes and twenty-five Nepalese barley accessions have three and more stripe rust resistance genes. Poor relationship between rust resistance gene and field disease may be due to the less relationship between the pathogen type on field and rust resistance genes detected using SSR markers on Nepalese barley germplasm.
Link for e-copy: http://elibrary.narc.gov.np/?r=1491 Molecular screening for rust resistance characteristics in Nepalese barley germplasm. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Sah, Bindeshwar P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 35-43 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'stripe rust', 'Puccinia', 'resistant', 'durable', 'gene' Abstract: Leaf rust ( caused by Puccinia hordei) and stripe/yellow rust ( caused by P. striformis fsp. hordei and P. striformis fsp. tritici) are the major problem for barley cultivation in Nepal. Durable resistant variety of the barley is highly important for this country. Barley rust resistance is controlled by many major genes and quantitative trait loci (QTLs) and pyramiding these genes into varieties is the most important barley breeding strategies in Nepal. Molecular marker assisted breeding (MAB) is the only option for this. Marker assisted screening is the primary stage to initiate the MAB. One hundred and nine Nepalese barley accessions were screened using six and eleven SSR markers linked to leaf rust and stripe rust resistance genes respectively. Bonus and Local Jau was used as the resistant and susceptible check respectively. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR-P and QTL on 5HS was detected on Nepalese barley accessions using respective SSR markers. Similarly, stripe rust resistance genes QTL on lH, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were detected. Eight Nepalese barley accessions showed presence of three and more leaf rust resistant genes and twenty-five Nepalese barley accessions have three and more stripe rust resistance genes. Poor relationship between rust resistance gene and field disease may be due to the less relationship between the pathogen type on field and rust resistance genes detected using SSR markers on Nepalese barley germplasm.
Link for e-copy: http://elibrary.narc.gov.np/?r=1491 E-copies
http://nkcs.org.np/narc/dl/?r=1491URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 18-22 p. Languages : English (eng) Keywords: 'durable', 'resistant', 'susceptible', 'pathotype', 'pyramiding' Abstract: Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on the availability and use of resistant gene donors. Ninety six Nepalese rice accessions were screened using eight SSR markers and one STS marker for presence and absence of BLB resistance gene. We detected BLB resistance gene Xa-10 on two accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-10 on five accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that different BLB pathotypes are active in Nepalese rice field and need of gene pyramiding for durable resistance.
Link for e-copy: http://elibrary.narc.gov.np/?r=1487 Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 18-22 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'durable', 'resistant', 'susceptible', 'pathotype', 'pyramiding' Abstract: Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on the availability and use of resistant gene donors. Ninety six Nepalese rice accessions were screened using eight SSR markers and one STS marker for presence and absence of BLB resistance gene. We detected BLB resistance gene Xa-10 on two accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-10 on five accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that different BLB pathotypes are active in Nepalese rice field and need of gene pyramiding for durable resistance.
Link for e-copy: http://elibrary.narc.gov.np/?r=1487 E-copies
http://nkcs.org.np/narc/dl/?r=1487URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Screening Nepalese rice germplasm for blast resistance characters using molecular markers. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 11-17 p. Languages : English (eng) Keywords: 'Magnaporthe' 'resistant' 'susceptible' 'check' 'gene' Abstract: Blast (caused by Magnaporthe oryzae) is the major yield limiting biotic stress for rice farming in Nepal that is prevalent all over the country. Resistant variety for one location becomes susceptible to another location which shows the need of resistant gene pyramiding for yield stability. Identifying blast resistant genes in Nepalese rice germplasm and its use for varietal development is very important. Ninety two Nepalese rice accessions were screened using 12 SSR markers for presence of different blast resistant genes. IR-64 and Sabitri were used as resistant check while Masuli and Jumli Marshi were used as susceptible check. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t) 1, Pi-z, Pi-a, Pi-k, Pi-yl (t) and Pi-44 were detected on seven, seven, nine, 11, 20 and 28 rice accessions respectively. Eighteen rice accessions showed presence Pi-b and Pi-g(t), 22 rice accessions showed presence of Pi-29 and Pi-11; and two rice accessions showed presence of Pi-ta and Pi20(t). Nepalese rice gene pool doesn't contain resistant gene Pi-d(t)2 and it needs to incorporate from exotic sources. Therefore, many Nepalese rice landraces can be used on marker assisted gene pyramiding program for development of durable blast resistance varieties.
Link for e-copy: http://elibrary.narc.gov.np/?r=1484 Screening Nepalese rice germplasm for blast resistance characters using molecular markers. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 11-17 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'Magnaporthe' 'resistant' 'susceptible' 'check' 'gene' Abstract: Blast (caused by Magnaporthe oryzae) is the major yield limiting biotic stress for rice farming in Nepal that is prevalent all over the country. Resistant variety for one location becomes susceptible to another location which shows the need of resistant gene pyramiding for yield stability. Identifying blast resistant genes in Nepalese rice germplasm and its use for varietal development is very important. Ninety two Nepalese rice accessions were screened using 12 SSR markers for presence of different blast resistant genes. IR-64 and Sabitri were used as resistant check while Masuli and Jumli Marshi were used as susceptible check. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t) 1, Pi-z, Pi-a, Pi-k, Pi-yl (t) and Pi-44 were detected on seven, seven, nine, 11, 20 and 28 rice accessions respectively. Eighteen rice accessions showed presence Pi-b and Pi-g(t), 22 rice accessions showed presence of Pi-29 and Pi-11; and two rice accessions showed presence of Pi-ta and Pi20(t). Nepalese rice gene pool doesn't contain resistant gene Pi-d(t)2 and it needs to incorporate from exotic sources. Therefore, many Nepalese rice landraces can be used on marker assisted gene pyramiding program for development of durable blast resistance varieties.
Link for e-copy: http://elibrary.narc.gov.np/?r=1484 E-copies
http://nkcs.org.np/narc/dl/?r=1484URL
