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Author Khatiwada, Shambhu P. |
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Refine your searchEvaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. / Madan Raj Bhatta
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Evaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 6-10 p. Languages : English (eng) Keywords: lesion; severity; incidence; resistant; susceptible; Abstract: Rice diseases are the major problem for rice production and blast is one of them. Development of the resistance varieties instead of using chemical and botanicals to treat the rice blast disease in field is most important in Nepalese context. Therefore, identification of resistance rice accessions from Nepalese rice germplasrn collection is very important for development of blast resistance rice varieties. One hundred and forty four Nepalese rice landraces were screened for their response on blast pathogen in natural condition at Khumaltar, Lalitpur, Nepal. Blast lesion type, disease severity, disease incidence and disease damage were recorded on 25 days old rice seedling. Local rice varieties Jumli Marshi and Masuli were used as susceptible check whereas Sabitri was used as resistant check. One hundred and nine rice landraces showed resistance reaction with blast. Similarly, sixty four rice landraces showed no blast disease symptom during seedling stage. Variation on origin of rice landraces may account for this differential reaction in blast disease development at seedling stage.
Link for e-copy: http://elibrary.narc.gov.np/?r=1482 Evaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 6-10 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: lesion; severity; incidence; resistant; susceptible; Abstract: Rice diseases are the major problem for rice production and blast is one of them. Development of the resistance varieties instead of using chemical and botanicals to treat the rice blast disease in field is most important in Nepalese context. Therefore, identification of resistance rice accessions from Nepalese rice germplasrn collection is very important for development of blast resistance rice varieties. One hundred and forty four Nepalese rice landraces were screened for their response on blast pathogen in natural condition at Khumaltar, Lalitpur, Nepal. Blast lesion type, disease severity, disease incidence and disease damage were recorded on 25 days old rice seedling. Local rice varieties Jumli Marshi and Masuli were used as susceptible check whereas Sabitri was used as resistant check. One hundred and nine rice landraces showed resistance reaction with blast. Similarly, sixty four rice landraces showed no blast disease symptom during seedling stage. Variation on origin of rice landraces may account for this differential reaction in blast disease development at seedling stage.
Link for e-copy: http://elibrary.narc.gov.np/?r=1482 E-copies
http://nkcs.org.np/narc/dl/?r=1482URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Molecular screening on rice and barley: a gateway to marker assisted selection. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Sah, Bindeshwar P., Author ; Khatiwada, Shambhu P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 1-5 p. Languages : English (eng) Keywords: abiotic; biotic; rust; blast; BLB; Abstract: Rice is the principle food crop of Nepal. Similarly, barley is another important crop in mountain areas of the Nepal. Increased yield productivity and yield stability of these two crops are always challenging in context of the unpredictable climate change. Nepal always lacks suitable cultivars in these crops which can challenge the biotic and abiotic threats from the climate change. Blast and bacterial blight resistant and submergence tolerant rice varieties; and leaf rust and stripe rust resistant barley varieties are highly demanding in Nepalese fanning community. Nepalese rice and barley landraces have many important genes for biotic and abiotic stress resistance. Therefore, we used 144 rice accessions for blast and bacterial leaf blight (BLB) resistance in rice, 313 rice accessions for flash flood tolerance and 241 barley accessions for leaf and yellow/stripe rust resistance characteristics for agro morphological and physiological screening during 2011/12 in Nepal Agriculture Research Council Farm Khumaltar, Lalitpur. Similarly, we have used 92 rice accessions for blast and 94 accessions for BLB resistance in rice, 46 accessions for flash flood tolerance in rice and 109 accessions for leaf and yellow/stripe rust resistance in barley for molecular screening based on their morphological and physiological screening. We have used six microsatellite (SSR) markers for leaf rust and 11 SSR markers for stripe rust resistance in barley, 12 SSR markers for Blast and eight SSR markers for BLB resistance in rice and eight SSR markers for flash flood tolerance characteristics in rice. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t)l, Pi-z, Pi-a, Pi-k, Pi-yl(t), Pi-44, Pi-b, Pi-g(t), Pi-29, Pi-11, Pi-ta and Pi20(t) were detected among Nepalese rice gene pool. Similarly, BLB resistance genes Xa-10, Xa-13, Xa-7, Xa-10, Xa-3, Xa-4, Xa-5, Xa-8 were detected. Presence of SublA-1 and SNORKEL genes also found in Nepalese rice landraces. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR• p and QTL on 5HS was detected on Nepalese barley accessions. Similarly, barley stripe rust resistance genes QTL on 1 H, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were also detected. This output is highly important for use of Nepalese rice and barley germplasm as donor parents for marker assisted breeding programs.
Link for e-copy: http://elibrary.narc.gov.np/?r=1481 Molecular screening on rice and barley: a gateway to marker assisted selection. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Sah, Bindeshwar P., Author ; Khatiwada, Shambhu P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 1-5 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: abiotic; biotic; rust; blast; BLB; Abstract: Rice is the principle food crop of Nepal. Similarly, barley is another important crop in mountain areas of the Nepal. Increased yield productivity and yield stability of these two crops are always challenging in context of the unpredictable climate change. Nepal always lacks suitable cultivars in these crops which can challenge the biotic and abiotic threats from the climate change. Blast and bacterial blight resistant and submergence tolerant rice varieties; and leaf rust and stripe rust resistant barley varieties are highly demanding in Nepalese fanning community. Nepalese rice and barley landraces have many important genes for biotic and abiotic stress resistance. Therefore, we used 144 rice accessions for blast and bacterial leaf blight (BLB) resistance in rice, 313 rice accessions for flash flood tolerance and 241 barley accessions for leaf and yellow/stripe rust resistance characteristics for agro morphological and physiological screening during 2011/12 in Nepal Agriculture Research Council Farm Khumaltar, Lalitpur. Similarly, we have used 92 rice accessions for blast and 94 accessions for BLB resistance in rice, 46 accessions for flash flood tolerance in rice and 109 accessions for leaf and yellow/stripe rust resistance in barley for molecular screening based on their morphological and physiological screening. We have used six microsatellite (SSR) markers for leaf rust and 11 SSR markers for stripe rust resistance in barley, 12 SSR markers for Blast and eight SSR markers for BLB resistance in rice and eight SSR markers for flash flood tolerance characteristics in rice. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t)l, Pi-z, Pi-a, Pi-k, Pi-yl(t), Pi-44, Pi-b, Pi-g(t), Pi-29, Pi-11, Pi-ta and Pi20(t) were detected among Nepalese rice gene pool. Similarly, BLB resistance genes Xa-10, Xa-13, Xa-7, Xa-10, Xa-3, Xa-4, Xa-5, Xa-8 were detected. Presence of SublA-1 and SNORKEL genes also found in Nepalese rice landraces. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR• p and QTL on 5HS was detected on Nepalese barley accessions. Similarly, barley stripe rust resistance genes QTL on 1 H, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were also detected. This output is highly important for use of Nepalese rice and barley germplasm as donor parents for marker assisted breeding programs.
Link for e-copy: http://elibrary.narc.gov.np/?r=1481 E-copies
http://nkcs.org.np/narc/dl/?r=1481URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Variation on rust resistance characteristics on Nepalese barley germplasm. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 31-34 p. Languages : English (eng) Keywords: 'Puccinia', 'resistant', 'susceptible', 'rust', 'gene' Abstract: Barley is the major crop for the people living in the high hills and mountainous region of the Nepal. Rust is the major production threat for barley cultivation. However, a lot of variation can be observed on Nepalese barley accessions with respect to rust resistance characteristics. Two hundred and forty one barley accessions were screened for leaf rust ( caused by Puccinia hordei) and stripe rust ( caused by P. striformis f.sp. hordei) resistance characteristics on heading stage at Khumaltar, Lalitpur, Nepal during normal barley growing season 2011. Bonus (origin Sweden) and Local Jau (Nepalese landrace) were used as resistant and susceptible check respectively. Seventeen barley accessions showed leaf rust susceptibility but resistant with stripe rust. Thirty barley accessions showed resistant for both leaf rust and strip rust. Lower frequency of the leaf rust infection may be due to prevalent resistance gene for leaf rust resistant characteristics in Nepalese barley.
Link for e-copy: http://elibrary.narc.gov.np/?r=1490 Variation on rust resistance characteristics on Nepalese barley germplasm. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 31-34 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'Puccinia', 'resistant', 'susceptible', 'rust', 'gene' Abstract: Barley is the major crop for the people living in the high hills and mountainous region of the Nepal. Rust is the major production threat for barley cultivation. However, a lot of variation can be observed on Nepalese barley accessions with respect to rust resistance characteristics. Two hundred and forty one barley accessions were screened for leaf rust ( caused by Puccinia hordei) and stripe rust ( caused by P. striformis f.sp. hordei) resistance characteristics on heading stage at Khumaltar, Lalitpur, Nepal during normal barley growing season 2011. Bonus (origin Sweden) and Local Jau (Nepalese landrace) were used as resistant and susceptible check respectively. Seventeen barley accessions showed leaf rust susceptibility but resistant with stripe rust. Thirty barley accessions showed resistant for both leaf rust and strip rust. Lower frequency of the leaf rust infection may be due to prevalent resistance gene for leaf rust resistant characteristics in Nepalese barley.
Link for e-copy: http://elibrary.narc.gov.np/?r=1490 E-copies
http://nkcs.org.np/narc/dl/?r=1490URL
