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Author Resham B Amgai |
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Refine your searchEvaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. / Madan Raj Bhatta
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Evaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 6-10 p. Languages : English (eng) Keywords: lesion; severity; incidence; resistant; susceptible; Abstract: Rice diseases are the major problem for rice production and blast is one of them. Development of the resistance varieties instead of using chemical and botanicals to treat the rice blast disease in field is most important in Nepalese context. Therefore, identification of resistance rice accessions from Nepalese rice germplasrn collection is very important for development of blast resistance rice varieties. One hundred and forty four Nepalese rice landraces were screened for their response on blast pathogen in natural condition at Khumaltar, Lalitpur, Nepal. Blast lesion type, disease severity, disease incidence and disease damage were recorded on 25 days old rice seedling. Local rice varieties Jumli Marshi and Masuli were used as susceptible check whereas Sabitri was used as resistant check. One hundred and nine rice landraces showed resistance reaction with blast. Similarly, sixty four rice landraces showed no blast disease symptom during seedling stage. Variation on origin of rice landraces may account for this differential reaction in blast disease development at seedling stage.
Link for e-copy: http://elibrary.narc.gov.np/?r=1482 Evaluation of Nepalese rice germ plasm for blast resistance at Khumaltar, Lalitpur, Nepal. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 6-10 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: lesion; severity; incidence; resistant; susceptible; Abstract: Rice diseases are the major problem for rice production and blast is one of them. Development of the resistance varieties instead of using chemical and botanicals to treat the rice blast disease in field is most important in Nepalese context. Therefore, identification of resistance rice accessions from Nepalese rice germplasrn collection is very important for development of blast resistance rice varieties. One hundred and forty four Nepalese rice landraces were screened for their response on blast pathogen in natural condition at Khumaltar, Lalitpur, Nepal. Blast lesion type, disease severity, disease incidence and disease damage were recorded on 25 days old rice seedling. Local rice varieties Jumli Marshi and Masuli were used as susceptible check whereas Sabitri was used as resistant check. One hundred and nine rice landraces showed resistance reaction with blast. Similarly, sixty four rice landraces showed no blast disease symptom during seedling stage. Variation on origin of rice landraces may account for this differential reaction in blast disease development at seedling stage.
Link for e-copy: http://elibrary.narc.gov.np/?r=1482 E-copies
http://nkcs.org.np/narc/dl/?r=1482URL
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Title : Floral trait variation in Nepalese wild and cultivated rice Material Type: printed text Authors: Resham B Amgai, Editor Publisher: Hardinath, Janakpur : National Rice Research Programme Publication Date: 2010 Pagination: 132-141 p. Abstract: Floral traits influence rice diversity, plasticity as well as its evolution and gene flow. Floral trait variation in Nepalese rice was
observed during 2006 at RARS-Lumle. They were evaluated at green house condition. 6 improved rice varieties and 31 rice
landraces were collected from terai and hill regions of Nepal. Similarly, 12 rice accessions (including male sterile) were collected
from IRRI and RARS-Lumle. 01yza rufipogon. 0. officinalis and 0. nivara (two accessions) were also observed during this study.
Higher variability was observed for glume opening angle, glume opening duration, stigma exertion, flag leaf angle, flag leaf
character, flag leaf length-breadth ratio, plant height, panicle length, panicle type, panicle exertion, spikelet fertility, pollen fertility,
stigma surface, style length, filament length, anther size and pollen diameter. 0. rufipogon was distinct with other rice while 0 .
nivara was found similar in many characters with landraces. The upland rice landraces Linde and Thulo Begani falls in same group
with cold tolerant rice Macchapuchre-3 and Chandannath-3 respectively. Glume opening angle showed good correlation with panicle
length {r-0.496, p<O.OOO), stigma exertion {r-0.472, p<O.OO I), stigma surface (r-0.386, p<0.007), filament length {r-0.3 18,
p<0.028) and anther size {r-0.508, p<O.OOO).Link for e-copy: http://elibrary.narc.gov.np/?r=1011 Floral trait variation in Nepalese wild and cultivated rice [printed text] / Resham B Amgai, Editor . - Hardinath, Janakpur : National Rice Research Programme, 2010 . - 132-141 p.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Abstract: Floral traits influence rice diversity, plasticity as well as its evolution and gene flow. Floral trait variation in Nepalese rice was
observed during 2006 at RARS-Lumle. They were evaluated at green house condition. 6 improved rice varieties and 31 rice
landraces were collected from terai and hill regions of Nepal. Similarly, 12 rice accessions (including male sterile) were collected
from IRRI and RARS-Lumle. 01yza rufipogon. 0. officinalis and 0. nivara (two accessions) were also observed during this study.
Higher variability was observed for glume opening angle, glume opening duration, stigma exertion, flag leaf angle, flag leaf
character, flag leaf length-breadth ratio, plant height, panicle length, panicle type, panicle exertion, spikelet fertility, pollen fertility,
stigma surface, style length, filament length, anther size and pollen diameter. 0. rufipogon was distinct with other rice while 0 .
nivara was found similar in many characters with landraces. The upland rice landraces Linde and Thulo Begani falls in same group
with cold tolerant rice Macchapuchre-3 and Chandannath-3 respectively. Glume opening angle showed good correlation with panicle
length {r-0.496, p<O.OOO), stigma exertion {r-0.472, p<O.OO I), stigma surface (r-0.386, p<0.007), filament length {r-0.3 18,
p<0.028) and anther size {r-0.508, p<O.OOO).Link for e-copy: http://elibrary.narc.gov.np/?r=1011 E-copies
http://nkcs.org.np/narc/dl/?r=1011URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author ; Ojha, Bishnu R., Author ; Surya K. Ghimire, Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 23-30 p. Languages : English (eng) Keywords: 'elongation', 'hypoxia', 'SublA', 'flooding', 'SNORKEL' Abstract: Flooding is a notorious environmental stresses that limits the rice production in Asia significantly. Rice has a unique capacity to withstand the period of flooding environment through the activation of coordinated regulatory efforts at morphological, anatomical, biochemical and molecular level. Three hundred and thirteen diverse rice collections including selected landraces from Nepal were completely submerged for 14 days for this study. Rice accessions had diverse response to submergence with survival ity ranging from 0 to 100 % and differential elongation growth response. Presence of Sub 1 A-1 and SNORKEL genes were then monitored in the tolerant landraces. Four landraces; Bhaatsaar, Kariyaparewa pakha, Sauthari and Karangi and 2 landraces; NPGR 3501 and NPGR 3503 showed better response than FRl 3A and Kalamon 113 respectively. However, Kariyaparewa pakha and Sauthari were found lacking the presence of Sub 1 A-1 allele. In addition, eight landraces with Sub I A- I allele were found intolerant to submergence. However, the SPAD reading showed that FR 13A had the highest chlorophyll retention capacity. Thus, the detail morpho-physiological and molecular analysis showed that both the tolerant and intolerant landraces possessed the SublA-1 allele and they showed both escape and quiescence strategy to cope the flooding. Therefore, SublA-1 allele is not the only ones responsible for tolerance but there is the participation of other stress responsive factors.
Link for e-copy: http://elibrary.narc.gov.np/?r=1489 Identification of flood tolerant genes Sub lA and SNORKEL from Nepalese rice gene pool. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author ; Ojha, Bishnu R., Author ; Surya K. Ghimire, Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 23-30 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'elongation', 'hypoxia', 'SublA', 'flooding', 'SNORKEL' Abstract: Flooding is a notorious environmental stresses that limits the rice production in Asia significantly. Rice has a unique capacity to withstand the period of flooding environment through the activation of coordinated regulatory efforts at morphological, anatomical, biochemical and molecular level. Three hundred and thirteen diverse rice collections including selected landraces from Nepal were completely submerged for 14 days for this study. Rice accessions had diverse response to submergence with survival ity ranging from 0 to 100 % and differential elongation growth response. Presence of Sub 1 A-1 and SNORKEL genes were then monitored in the tolerant landraces. Four landraces; Bhaatsaar, Kariyaparewa pakha, Sauthari and Karangi and 2 landraces; NPGR 3501 and NPGR 3503 showed better response than FRl 3A and Kalamon 113 respectively. However, Kariyaparewa pakha and Sauthari were found lacking the presence of Sub 1 A-1 allele. In addition, eight landraces with Sub I A- I allele were found intolerant to submergence. However, the SPAD reading showed that FR 13A had the highest chlorophyll retention capacity. Thus, the detail morpho-physiological and molecular analysis showed that both the tolerant and intolerant landraces possessed the SublA-1 allele and they showed both escape and quiescence strategy to cope the flooding. Therefore, SublA-1 allele is not the only ones responsible for tolerance but there is the participation of other stress responsive factors.
Link for e-copy: http://elibrary.narc.gov.np/?r=1489 E-copies
http://nkcs.org.np/narc/dl/?r=1489URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Molecular screening for rust resistance characteristics in Nepalese barley germplasm. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Sah, Bindeshwar P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 35-43 p. Languages : English (eng) Keywords: 'stripe rust', 'Puccinia', 'resistant', 'durable', 'gene' Abstract: Leaf rust ( caused by Puccinia hordei) and stripe/yellow rust ( caused by P. striformis fsp. hordei and P. striformis fsp. tritici) are the major problem for barley cultivation in Nepal. Durable resistant variety of the barley is highly important for this country. Barley rust resistance is controlled by many major genes and quantitative trait loci (QTLs) and pyramiding these genes into varieties is the most important barley breeding strategies in Nepal. Molecular marker assisted breeding (MAB) is the only option for this. Marker assisted screening is the primary stage to initiate the MAB. One hundred and nine Nepalese barley accessions were screened using six and eleven SSR markers linked to leaf rust and stripe rust resistance genes respectively. Bonus and Local Jau was used as the resistant and susceptible check respectively. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR-P and QTL on 5HS was detected on Nepalese barley accessions using respective SSR markers. Similarly, stripe rust resistance genes QTL on lH, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were detected. Eight Nepalese barley accessions showed presence of three and more leaf rust resistant genes and twenty-five Nepalese barley accessions have three and more stripe rust resistance genes. Poor relationship between rust resistance gene and field disease may be due to the less relationship between the pathogen type on field and rust resistance genes detected using SSR markers on Nepalese barley germplasm.
Link for e-copy: http://elibrary.narc.gov.np/?r=1491 Molecular screening for rust resistance characteristics in Nepalese barley germplasm. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Sah, Bindeshwar P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 35-43 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'stripe rust', 'Puccinia', 'resistant', 'durable', 'gene' Abstract: Leaf rust ( caused by Puccinia hordei) and stripe/yellow rust ( caused by P. striformis fsp. hordei and P. striformis fsp. tritici) are the major problem for barley cultivation in Nepal. Durable resistant variety of the barley is highly important for this country. Barley rust resistance is controlled by many major genes and quantitative trait loci (QTLs) and pyramiding these genes into varieties is the most important barley breeding strategies in Nepal. Molecular marker assisted breeding (MAB) is the only option for this. Marker assisted screening is the primary stage to initiate the MAB. One hundred and nine Nepalese barley accessions were screened using six and eleven SSR markers linked to leaf rust and stripe rust resistance genes respectively. Bonus and Local Jau was used as the resistant and susceptible check respectively. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR-P and QTL on 5HS was detected on Nepalese barley accessions using respective SSR markers. Similarly, stripe rust resistance genes QTL on lH, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were detected. Eight Nepalese barley accessions showed presence of three and more leaf rust resistant genes and twenty-five Nepalese barley accessions have three and more stripe rust resistance genes. Poor relationship between rust resistance gene and field disease may be due to the less relationship between the pathogen type on field and rust resistance genes detected using SSR markers on Nepalese barley germplasm.
Link for e-copy: http://elibrary.narc.gov.np/?r=1491 E-copies
http://nkcs.org.np/narc/dl/?r=1491URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Molecular screening on rice and barley: a gateway to marker assisted selection. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Sah, Bindeshwar P., Author ; Khatiwada, Shambhu P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 1-5 p. Languages : English (eng) Keywords: abiotic; biotic; rust; blast; BLB; Abstract: Rice is the principle food crop of Nepal. Similarly, barley is another important crop in mountain areas of the Nepal. Increased yield productivity and yield stability of these two crops are always challenging in context of the unpredictable climate change. Nepal always lacks suitable cultivars in these crops which can challenge the biotic and abiotic threats from the climate change. Blast and bacterial blight resistant and submergence tolerant rice varieties; and leaf rust and stripe rust resistant barley varieties are highly demanding in Nepalese fanning community. Nepalese rice and barley landraces have many important genes for biotic and abiotic stress resistance. Therefore, we used 144 rice accessions for blast and bacterial leaf blight (BLB) resistance in rice, 313 rice accessions for flash flood tolerance and 241 barley accessions for leaf and yellow/stripe rust resistance characteristics for agro morphological and physiological screening during 2011/12 in Nepal Agriculture Research Council Farm Khumaltar, Lalitpur. Similarly, we have used 92 rice accessions for blast and 94 accessions for BLB resistance in rice, 46 accessions for flash flood tolerance in rice and 109 accessions for leaf and yellow/stripe rust resistance in barley for molecular screening based on their morphological and physiological screening. We have used six microsatellite (SSR) markers for leaf rust and 11 SSR markers for stripe rust resistance in barley, 12 SSR markers for Blast and eight SSR markers for BLB resistance in rice and eight SSR markers for flash flood tolerance characteristics in rice. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t)l, Pi-z, Pi-a, Pi-k, Pi-yl(t), Pi-44, Pi-b, Pi-g(t), Pi-29, Pi-11, Pi-ta and Pi20(t) were detected among Nepalese rice gene pool. Similarly, BLB resistance genes Xa-10, Xa-13, Xa-7, Xa-10, Xa-3, Xa-4, Xa-5, Xa-8 were detected. Presence of SublA-1 and SNORKEL genes also found in Nepalese rice landraces. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR• p and QTL on 5HS was detected on Nepalese barley accessions. Similarly, barley stripe rust resistance genes QTL on 1 H, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were also detected. This output is highly important for use of Nepalese rice and barley germplasm as donor parents for marker assisted breeding programs.
Link for e-copy: http://elibrary.narc.gov.np/?r=1481 Molecular screening on rice and barley: a gateway to marker assisted selection. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Sah, Bindeshwar P., Author ; Khatiwada, Shambhu P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 1-5 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: abiotic; biotic; rust; blast; BLB; Abstract: Rice is the principle food crop of Nepal. Similarly, barley is another important crop in mountain areas of the Nepal. Increased yield productivity and yield stability of these two crops are always challenging in context of the unpredictable climate change. Nepal always lacks suitable cultivars in these crops which can challenge the biotic and abiotic threats from the climate change. Blast and bacterial blight resistant and submergence tolerant rice varieties; and leaf rust and stripe rust resistant barley varieties are highly demanding in Nepalese fanning community. Nepalese rice and barley landraces have many important genes for biotic and abiotic stress resistance. Therefore, we used 144 rice accessions for blast and bacterial leaf blight (BLB) resistance in rice, 313 rice accessions for flash flood tolerance and 241 barley accessions for leaf and yellow/stripe rust resistance characteristics for agro morphological and physiological screening during 2011/12 in Nepal Agriculture Research Council Farm Khumaltar, Lalitpur. Similarly, we have used 92 rice accessions for blast and 94 accessions for BLB resistance in rice, 46 accessions for flash flood tolerance in rice and 109 accessions for leaf and yellow/stripe rust resistance in barley for molecular screening based on their morphological and physiological screening. We have used six microsatellite (SSR) markers for leaf rust and 11 SSR markers for stripe rust resistance in barley, 12 SSR markers for Blast and eight SSR markers for BLB resistance in rice and eight SSR markers for flash flood tolerance characteristics in rice. Blast resistant genes Pi-54, Pi-y2(t), Pi-d(t)l, Pi-z, Pi-a, Pi-k, Pi-yl(t), Pi-44, Pi-b, Pi-g(t), Pi-29, Pi-11, Pi-ta and Pi20(t) were detected among Nepalese rice gene pool. Similarly, BLB resistance genes Xa-10, Xa-13, Xa-7, Xa-10, Xa-3, Xa-4, Xa-5, Xa-8 were detected. Presence of SublA-1 and SNORKEL genes also found in Nepalese rice landraces. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR• p and QTL on 5HS was detected on Nepalese barley accessions. Similarly, barley stripe rust resistance genes QTL on 1 H, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were also detected. This output is highly important for use of Nepalese rice and barley germplasm as donor parents for marker assisted breeding programs.
Link for e-copy: http://elibrary.narc.gov.np/?r=1481 E-copies
http://nkcs.org.np/narc/dl/?r=1481URL
