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Author H.P Bimb |
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Refine your searchBuckwheat diversity in Nepal in the context of morphological, biochemical and Molecular analysis / H.P Bimb
in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.
Title : Buckwheat diversity in Nepal in the context of morphological, biochemical and Molecular analysis Material Type: printed text Authors: H.P Bimb, Author Pagination: 146 p. Languages : English (eng) Keywords: Biochemical, buckwheat, diversity, molecular analysis, morphology, Nepal Abstract: Landraces and wild relative of crop species are regarded as the indispensable gene sources for future genetic improvement. For effective conservation of genetic variation, it is necessary to identify the target population based on its genetic variation and sustainability in its habitat. Buckwheat (Fagopyrum spp) being grown in wide geographic variations of Nepal is especially important source of food for marginal farmers of hills and mountains………..
Link for e-copy: http://elibrary.narc.gov.np/?r=1076 Buckwheat diversity in Nepal in the context of morphological, biochemical and Molecular analysis [printed text] / H.P Bimb, Author . - [s.d.] . - 146 p.
in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.
Languages : English (eng)
Keywords: Biochemical, buckwheat, diversity, molecular analysis, morphology, Nepal Abstract: Landraces and wild relative of crop species are regarded as the indispensable gene sources for future genetic improvement. For effective conservation of genetic variation, it is necessary to identify the target population based on its genetic variation and sustainability in its habitat. Buckwheat (Fagopyrum spp) being grown in wide geographic variations of Nepal is especially important source of food for marginal farmers of hills and mountains………..
Link for e-copy: http://elibrary.narc.gov.np/?r=1076 E-copies
http://nkcs.org.np/narc/dl/?r=1076URL
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Title : Classification of Nepalese rice cultivars based on Isozymes Material Type: printed text Authors: B.K. Joshi ; H.P Bimb, Author Pagination: 175-187 p. Languages : English (eng) Keywords: Varietal classification ; isozyme rice cultivars Abstract: Classification of large variation within Oryza sativa has been importance for breeders and
geneticists. A total of 39 Nepalese rice cultivars including one non-released, Fuji-102 were
classified and evaluated their characteristics features of eight isozyme systems, Alcohol
dehydrogenase (ADH), Acid phosphatase (ACP), Esterase (EST), lsocitrate dehydrogenase
(IDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Peroxidase (POX) and Shikimate
dehydrogenase (SDH). Plumules and coleoptiles of 3-5 seedlings of each cultivar were used to
extract enzyme tfSing Sodium ascorbate extraction buffer at pH 7.4. These were electrophoresed
and enzymes were detected after staining in an appropriate staining mixture. Cluster and
principal component analyses were applied on binary data matrix. Zymograms were presented
separately for each enzyme and each varietal type. A to
tal of 28, 19 and 23 distinct electromorphs were used as iso:;ymic descriptors for indica,
intermediate and japonica varietal types respectively. Multivariate analysis grouped these
cultivars into 3. These were reclassified in indica (22), intermediate (3} andjaponica (14) varietal
types. There were sixteen loci coding eight isozyme systems. All loci were f ound in indica group,
/4 loci in intermediate and 15 in japonica. Most of the enzyme systems expressed distinct
zymogramsfor indica andjaponica. Highest zymotypes were expressed by POX Loci Mdh-3 and
Sdh-1 have highest number of alleles within indica type, Pox-] within intermediate and Sdh-1 and
Pox-/ withinjaponica types. Locus Mdh-2 was not noticed in intermediate andjaponica groups.
Loci Mdh-3, Est-2 and Sdh-1 .showed hfg~est gene diversity within indica, intermediate and
j aponica rypes respectively. Among the four multilocus -enzymes, three were polymorphic. All loci
except Pox-4 were polymorhpic in intermediate varietal types. all loci were polymorhpihc in
indica and except Pox-3 and Adh:-1 all loci were polymorphic considering null allele injaponica.
This isozymic variation within and between varietal types can be itseful for further study and the
isozyme profiles of each cultivar can be used to trace their identity.Link for e-copy: http://elibrary.narc.gov.np/?r=1100 Classification of Nepalese rice cultivars based on Isozymes [printed text] / B.K. Joshi ; H.P Bimb, Author . - [s.d.] . - 175-187 p.
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Languages : English (eng)
Keywords: Varietal classification ; isozyme rice cultivars Abstract: Classification of large variation within Oryza sativa has been importance for breeders and
geneticists. A total of 39 Nepalese rice cultivars including one non-released, Fuji-102 were
classified and evaluated their characteristics features of eight isozyme systems, Alcohol
dehydrogenase (ADH), Acid phosphatase (ACP), Esterase (EST), lsocitrate dehydrogenase
(IDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Peroxidase (POX) and Shikimate
dehydrogenase (SDH). Plumules and coleoptiles of 3-5 seedlings of each cultivar were used to
extract enzyme tfSing Sodium ascorbate extraction buffer at pH 7.4. These were electrophoresed
and enzymes were detected after staining in an appropriate staining mixture. Cluster and
principal component analyses were applied on binary data matrix. Zymograms were presented
separately for each enzyme and each varietal type. A to
tal of 28, 19 and 23 distinct electromorphs were used as iso:;ymic descriptors for indica,
intermediate and japonica varietal types respectively. Multivariate analysis grouped these
cultivars into 3. These were reclassified in indica (22), intermediate (3} andjaponica (14) varietal
types. There were sixteen loci coding eight isozyme systems. All loci were f ound in indica group,
/4 loci in intermediate and 15 in japonica. Most of the enzyme systems expressed distinct
zymogramsfor indica andjaponica. Highest zymotypes were expressed by POX Loci Mdh-3 and
Sdh-1 have highest number of alleles within indica type, Pox-] within intermediate and Sdh-1 and
Pox-/ withinjaponica types. Locus Mdh-2 was not noticed in intermediate andjaponica groups.
Loci Mdh-3, Est-2 and Sdh-1 .showed hfg~est gene diversity within indica, intermediate and
j aponica rypes respectively. Among the four multilocus -enzymes, three were polymorphic. All loci
except Pox-4 were polymorhpic in intermediate varietal types. all loci were polymorhpihc in
indica and except Pox-3 and Adh:-1 all loci were polymorphic considering null allele injaponica.
This isozymic variation within and between varietal types can be itseful for further study and the
isozyme profiles of each cultivar can be used to trace their identity.Link for e-copy: http://elibrary.narc.gov.np/?r=1100 E-copies
http://nkcs.org.np/narc/dl/?r=1100URL
Title : Evaluation of rice lines for resistance to bacterial leaf blight Material Type: printed text Authors: B. Chaudhary ; Chaudhary, R.N., Author ; H.P Bimb, Author Pagination: 136-144 p. Languages : English (eng) Abstract: A total of 220 rice genotypes included in National Rice Bacterial leaf Blight Nursery were
evaluated for bacterial leaf blight resistance in the 1996 wet season. The screening nursery
was conducted at Tarahara, Hardinath, Panvanipur and Bhairahawa. None of the test
ge.notypes showed resistant reaction to the disease over all the locations. Disease intensity at
Tarahara was very high. Excluding Tarahara location, 33 rice lines rated resistant reaction
over the rest three locations ..Link for e-copy: http://elibrary.narc.gov.np/?r=493 Evaluation of rice lines for resistance to bacterial leaf blight [printed text] / B. Chaudhary ; Chaudhary, R.N., Author ; H.P Bimb, Author . - [s.d.] . - 136-144 p.
Languages : English (eng)
Abstract: A total of 220 rice genotypes included in National Rice Bacterial leaf Blight Nursery were
evaluated for bacterial leaf blight resistance in the 1996 wet season. The screening nursery
was conducted at Tarahara, Hardinath, Panvanipur and Bhairahawa. None of the test
ge.notypes showed resistant reaction to the disease over all the locations. Disease intensity at
Tarahara was very high. Excluding Tarahara location, 33 rice lines rated resistant reaction
over the rest three locations ..Link for e-copy: http://elibrary.narc.gov.np/?r=493 E-copies
http://nkcs.org.np/narc/dl/?r=493URL
Title : Evaluation of wheat genotypes for disease resistance, 1997/98 Material Type: printed text Authors: D.B. Gharti, Author ; Bhandari, D., Author ; H.P Bimb, Author ; Bhatta, M.R., Author Pagination: 163-169 p. Languages : English (eng) Link for e-copy: http://elibrary.narc.gov.np/?r=2755 Evaluation of wheat genotypes for disease resistance, 1997/98 [printed text] / D.B. Gharti, Author ; Bhandari, D., Author ; H.P Bimb, Author ; Bhatta, M.R., Author . - [s.d.] . - 163-169 p.
Languages : English (eng)
Link for e-copy: http://elibrary.narc.gov.np/?r=2755
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Title : Isozyme variations in fine and aromatic rice genotypes Material Type: printed text Authors: H.P Bimb ; Koirala, K.B., Author ; Sah, R.P., Author ; N.K Lal, Author ; B.P Sah, Author Pagination: 145-155 p. Languages : English (eng) Abstract: Rice is the main staple food crop in Nepal. Fine and aromatic rice varieties have
significant commercial value. Allozyine electrophoresis has been a reliable technique in
estimating the genetic variation in crops. We surveyed isozyme variation of 24 fine and ·
aromatic rice_ genotypes collected from Nepal including Jetho budho land race as a
check. Five enzyme system5-were analyzed for allozyme variability that detected distinct
10 isozyme loci and 15 alleles including null alleles. The polymorphic enzyme systems:
esterase revealed 8 phenotypes following 6- phosphoglutanate dehydrogenase and
peroxidase each with 3 phenotypes an_d alcohol dehydrogenase and asparate
aminotransferase each with 2 phenotypes based on the combination of presence and
absence of alleles. T}J,e results indicated diversity within and between population of test
genotypes. Dendrogram did not indicate any clear pattern of divisions of these
populations into definite clusters. However 3 groups were detected at 84% . of
dissimilarity among 24 populations. Nepalese varieties Rato Basmati, Pahenle and
Jetho Budho grouped in a separate cluster in the dendrogram.Link for e-copy: http://elibrary.narc.gov.np/?r=1096 Isozyme variations in fine and aromatic rice genotypes [printed text] / H.P Bimb ; Koirala, K.B., Author ; Sah, R.P., Author ; N.K Lal, Author ; B.P Sah, Author . - [s.d.] . - 145-155 p.
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Languages : English (eng)
Abstract: Rice is the main staple food crop in Nepal. Fine and aromatic rice varieties have
significant commercial value. Allozyine electrophoresis has been a reliable technique in
estimating the genetic variation in crops. We surveyed isozyme variation of 24 fine and ·
aromatic rice_ genotypes collected from Nepal including Jetho budho land race as a
check. Five enzyme system5-were analyzed for allozyme variability that detected distinct
10 isozyme loci and 15 alleles including null alleles. The polymorphic enzyme systems:
esterase revealed 8 phenotypes following 6- phosphoglutanate dehydrogenase and
peroxidase each with 3 phenotypes an_d alcohol dehydrogenase and asparate
aminotransferase each with 2 phenotypes based on the combination of presence and
absence of alleles. T}J,e results indicated diversity within and between population of test
genotypes. Dendrogram did not indicate any clear pattern of divisions of these
populations into definite clusters. However 3 groups were detected at 84% . of
dissimilarity among 24 populations. Nepalese varieties Rato Basmati, Pahenle and
Jetho Budho grouped in a separate cluster in the dendrogram.Link for e-copy: http://elibrary.narc.gov.np/?r=1096 E-copies
http://nkcs.org.np/narc/dl/?r=1096URL
