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Author B.K. Joshi |
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Refine your searchCharacterization of finger millet (Eleusine coracana Gaertu.) Germplasm with agro Morphological makers / Bhattarai M
Title : Characterization of finger millet (Eleusine coracana Gaertu.) Germplasm with agro Morphological makers Material Type: printed text Authors: Bhattarai M, Editor ; Ghimire, K.H. ; B.K. Joshi, Author ; Bhatta, M.R., Author Pagination: 184-189 p. Keywords: Finger millet ; agro-morphological makers diversity traits Abstract: Finger millet [Eleusine coracana (L.) Gaertn.] is an important crop of the millets group
cultivated in terai and hills of Nepal. A total of 537 accessions of finger millet were
characterized during the years 2010 and 2012 at National Agriculture Genetic Resources
Centre (NAGRC), Khumaltar, Lalitpur, Nepal. Standard characterization descriptors were
used to record 12 quantitative and 7 qualitative characters. Different measures of variation
like mean, standard deviation, range, Shannon-Weaver diversity indices (H') were used for
the assessment of diversity between the accessions. Highest degree of diversity was
observed for number of leaves (0.906) followed by leaf sheath length (0.902), ear exertion
(0.901), blade length of flag leaf (0.893), leaf blade width (0.886), heading days (0.885),
plant height (0.879), leaf blade length (0.872), number of productive tillers (0.860), finger
length (0.841) and ear size (0.830). With the R2 value of 0.6, 14 clusters were observed
based on Euclidean's distance (quantitative traits) whereas with the R2 value of 0.8, 12
clusters were observed based on Gower's distance (quantitative and qualitative traits).
Three principle components explained 96.13% of the total variation in the collection. Ear
shape (0.99) is the most important variable in principle component 1.Characterization of finger millet (Eleusine coracana Gaertu.) Germplasm with agro Morphological makers [printed text] / Bhattarai M, Editor ; Ghimire, K.H. ; B.K. Joshi, Author ; Bhatta, M.R., Author . - [s.d.] . - 184-189 p.
Keywords: Finger millet ; agro-morphological makers diversity traits Abstract: Finger millet [Eleusine coracana (L.) Gaertn.] is an important crop of the millets group
cultivated in terai and hills of Nepal. A total of 537 accessions of finger millet were
characterized during the years 2010 and 2012 at National Agriculture Genetic Resources
Centre (NAGRC), Khumaltar, Lalitpur, Nepal. Standard characterization descriptors were
used to record 12 quantitative and 7 qualitative characters. Different measures of variation
like mean, standard deviation, range, Shannon-Weaver diversity indices (H') were used for
the assessment of diversity between the accessions. Highest degree of diversity was
observed for number of leaves (0.906) followed by leaf sheath length (0.902), ear exertion
(0.901), blade length of flag leaf (0.893), leaf blade width (0.886), heading days (0.885),
plant height (0.879), leaf blade length (0.872), number of productive tillers (0.860), finger
length (0.841) and ear size (0.830). With the R2 value of 0.6, 14 clusters were observed
based on Euclidean's distance (quantitative traits) whereas with the R2 value of 0.8, 12
clusters were observed based on Gower's distance (quantitative and qualitative traits).
Three principle components explained 96.13% of the total variation in the collection. Ear
shape (0.99) is the most important variable in principle component 1.
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Title : Classification of Nepalese rice cultivars based on Isozymes Material Type: printed text Authors: B.K. Joshi ; H.P Bimb, Author Pagination: 175-187 p. Languages : English (eng) Keywords: Varietal classification ; isozyme rice cultivars Abstract: Classification of large variation within Oryza sativa has been importance for breeders and
geneticists. A total of 39 Nepalese rice cultivars including one non-released, Fuji-102 were
classified and evaluated their characteristics features of eight isozyme systems, Alcohol
dehydrogenase (ADH), Acid phosphatase (ACP), Esterase (EST), lsocitrate dehydrogenase
(IDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Peroxidase (POX) and Shikimate
dehydrogenase (SDH). Plumules and coleoptiles of 3-5 seedlings of each cultivar were used to
extract enzyme tfSing Sodium ascorbate extraction buffer at pH 7.4. These were electrophoresed
and enzymes were detected after staining in an appropriate staining mixture. Cluster and
principal component analyses were applied on binary data matrix. Zymograms were presented
separately for each enzyme and each varietal type. A to
tal of 28, 19 and 23 distinct electromorphs were used as iso:;ymic descriptors for indica,
intermediate and japonica varietal types respectively. Multivariate analysis grouped these
cultivars into 3. These were reclassified in indica (22), intermediate (3} andjaponica (14) varietal
types. There were sixteen loci coding eight isozyme systems. All loci were f ound in indica group,
/4 loci in intermediate and 15 in japonica. Most of the enzyme systems expressed distinct
zymogramsfor indica andjaponica. Highest zymotypes were expressed by POX Loci Mdh-3 and
Sdh-1 have highest number of alleles within indica type, Pox-] within intermediate and Sdh-1 and
Pox-/ withinjaponica types. Locus Mdh-2 was not noticed in intermediate andjaponica groups.
Loci Mdh-3, Est-2 and Sdh-1 .showed hfg~est gene diversity within indica, intermediate and
j aponica rypes respectively. Among the four multilocus -enzymes, three were polymorphic. All loci
except Pox-4 were polymorhpic in intermediate varietal types. all loci were polymorhpihc in
indica and except Pox-3 and Adh:-1 all loci were polymorphic considering null allele injaponica.
This isozymic variation within and between varietal types can be itseful for further study and the
isozyme profiles of each cultivar can be used to trace their identity.Link for e-copy: http://elibrary.narc.gov.np/?r=1100 Classification of Nepalese rice cultivars based on Isozymes [printed text] / B.K. Joshi ; H.P Bimb, Author . - [s.d.] . - 175-187 p.
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Languages : English (eng)
Keywords: Varietal classification ; isozyme rice cultivars Abstract: Classification of large variation within Oryza sativa has been importance for breeders and
geneticists. A total of 39 Nepalese rice cultivars including one non-released, Fuji-102 were
classified and evaluated their characteristics features of eight isozyme systems, Alcohol
dehydrogenase (ADH), Acid phosphatase (ACP), Esterase (EST), lsocitrate dehydrogenase
(IDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Peroxidase (POX) and Shikimate
dehydrogenase (SDH). Plumules and coleoptiles of 3-5 seedlings of each cultivar were used to
extract enzyme tfSing Sodium ascorbate extraction buffer at pH 7.4. These were electrophoresed
and enzymes were detected after staining in an appropriate staining mixture. Cluster and
principal component analyses were applied on binary data matrix. Zymograms were presented
separately for each enzyme and each varietal type. A to
tal of 28, 19 and 23 distinct electromorphs were used as iso:;ymic descriptors for indica,
intermediate and japonica varietal types respectively. Multivariate analysis grouped these
cultivars into 3. These were reclassified in indica (22), intermediate (3} andjaponica (14) varietal
types. There were sixteen loci coding eight isozyme systems. All loci were f ound in indica group,
/4 loci in intermediate and 15 in japonica. Most of the enzyme systems expressed distinct
zymogramsfor indica andjaponica. Highest zymotypes were expressed by POX Loci Mdh-3 and
Sdh-1 have highest number of alleles within indica type, Pox-] within intermediate and Sdh-1 and
Pox-/ withinjaponica types. Locus Mdh-2 was not noticed in intermediate andjaponica groups.
Loci Mdh-3, Est-2 and Sdh-1 .showed hfg~est gene diversity within indica, intermediate and
j aponica rypes respectively. Among the four multilocus -enzymes, three were polymorphic. All loci
except Pox-4 were polymorhpic in intermediate varietal types. all loci were polymorhpihc in
indica and except Pox-3 and Adh:-1 all loci were polymorphic considering null allele injaponica.
This isozymic variation within and between varietal types can be itseful for further study and the
isozyme profiles of each cultivar can be used to trace their identity.Link for e-copy: http://elibrary.narc.gov.np/?r=1100 E-copies
http://nkcs.org.np/narc/dl/?r=1100URL
Title : Ecogeographic and Genetic Diversity Analysis of Mango in Nepal Material Type: printed text Authors: Subedi, A, Author ; Bajracharya, J., Author ; Regmi, H.N., Author ; H. B. K. C, Author ; Gupta, S.R., Author ; B.K. Joshi, Author Pagination: 66-70 p. Languages : English (eng) Keywords: ccogcographic survey, genetic diversity, landraccs, diversity index Abstract: Mango is one of the important tropical fruits of Nepal ami occurs as a domesticated or ll'ild
entity in the complex of biotic community of the ecosystem. Wide ecological variation and
diverse needs of multi-ethnic comm1mities has enriched the country with genetic 1vealth of
diverse mango varieties and land races. The tropical plain, river gorge areas and sub-tropical
valleys are the regions that are rich in mango diversity. As a result, the global project on
"Strengthening scientific basis of in-situ consen•ation of agricultural biodiversity on-farm,
Nepal componellt" identified mango as a mandatol)' crop and under the project,
ecogeographic sun•ey and genetic di1•ersity was carried to measure the extent of diversity.
Siraha, Saptari, Dhading, Kavre, Parbat. Baglw1g and Dadeldlwra, the districts representing
the Terai and mid-hill ecosystems ll'ere surreyed for mango diversity. Fruit samples of 216
mango trees collected in ecogeographic sun•ey were evaluated for nineteen nwrplwlogical
characters of fruit to understand the genetic relationships. A mnge of \'(/riation e.risted in
varietalfrmit of mango with dijferellf shapes, co/ow; sizes,j7al'fmr (aroma) and taste u·ith 25-
47 coefficiellts of variation and 0.59-0.87 Shannon Weaver index. Principal compm1e11t
analysis of qualitative traits and quallfitative traits of fruits sholl'ed the existence of a
collfinuum diversity of mango in Ne1}(ll with no ecogeograpflic differentiation and qualiwtive
traits were of importance in determining tile groupings and relationships rif mcmgo \'Orieties
under study. The ecogeographic survey findings and genetic di1•ersity results were discussed
relative to the efficient utilization and c:onsen•ation.Link for e-copy: http://elibrary.narc.gov.np/?r=453 Ecogeographic and Genetic Diversity Analysis of Mango in Nepal [printed text] / Subedi, A, Author ; Bajracharya, J., Author ; Regmi, H.N., Author ; H. B. K. C, Author ; Gupta, S.R., Author ; B.K. Joshi, Author . - [s.d.] . - 66-70 p.
Languages : English (eng)
Keywords: ccogcographic survey, genetic diversity, landraccs, diversity index Abstract: Mango is one of the important tropical fruits of Nepal ami occurs as a domesticated or ll'ild
entity in the complex of biotic community of the ecosystem. Wide ecological variation and
diverse needs of multi-ethnic comm1mities has enriched the country with genetic 1vealth of
diverse mango varieties and land races. The tropical plain, river gorge areas and sub-tropical
valleys are the regions that are rich in mango diversity. As a result, the global project on
"Strengthening scientific basis of in-situ consen•ation of agricultural biodiversity on-farm,
Nepal componellt" identified mango as a mandatol)' crop and under the project,
ecogeographic sun•ey and genetic di1•ersity was carried to measure the extent of diversity.
Siraha, Saptari, Dhading, Kavre, Parbat. Baglw1g and Dadeldlwra, the districts representing
the Terai and mid-hill ecosystems ll'ere surreyed for mango diversity. Fruit samples of 216
mango trees collected in ecogeographic sun•ey were evaluated for nineteen nwrplwlogical
characters of fruit to understand the genetic relationships. A mnge of \'(/riation e.risted in
varietalfrmit of mango with dijferellf shapes, co/ow; sizes,j7al'fmr (aroma) and taste u·ith 25-
47 coefficiellts of variation and 0.59-0.87 Shannon Weaver index. Principal compm1e11t
analysis of qualitative traits and quallfitative traits of fruits sholl'ed the existence of a
collfinuum diversity of mango in Ne1}(ll with no ecogeograpflic differentiation and qualiwtive
traits were of importance in determining tile groupings and relationships rif mcmgo \'Orieties
under study. The ecogeographic survey findings and genetic di1•ersity results were discussed
relative to the efficient utilization and c:onsen•ation.Link for e-copy: http://elibrary.narc.gov.np/?r=453 E-copies
http://nkcs.org.np/narc/dl/?r=453URL
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Title : Realities on Nepalese rice landraces Material Type: printed text Authors: RB Amgain ; B.K. Joshi, Author ; NP Adhikari, Author ; A Mudwari, Author ; MP Upadhayay, Author ; BK Baniya, Author Pagination: 418-423 p. Languages : English (eng) Abstract: Rice is economically, socially, and culturally important crop of Nepal. Landraces occupies
the significant role in Nepalese rice system. They are important rice genetic resources of the
count1y. However, they are declining day by day. Household survey and focus group
discussion was carried out in Zvadul besi area (Gorkha) d!lring Nov.-Dec. 2003. Focus
group discussion iiz Kachorwa (Bara) was also conducted during Jan. 2004. The purpose of
the study was to identify the changed diversity on rice landraces, and its cause. In case of
Kachorwa, Community Biodiversity Register (CBR), 2003 was used for our purpose. Genetic
erosion was also quantified using Guarino 's score (199 5). Though, the score for genetic
erosion is not high in Zydul besi (20), the inter-varietal diversity of rice landraces was found
decreasing. Zydul besi farmers were searching for rice varieties that have higher and stable
yield, but till now no modern varieties can compete with their landraces Aanpjhutte. No. and
area un:Jer landraces was found drastically decreased in Kachorwa since last fev.• years.
Despite of higher Guarino's score (75), In-situ conservation on agro-biodiversity project is
working effectively for consen,ation of rice landraces using different approach of
conservation. However, Kachonva farmers also start the introduction of hybrid rice.
Culturally important rice landraces were found cultivated by many households in small area
only. Since, rice landraces were most 1ieed to resource poor farmers, its replacement can be
effectively reduced by removal of its undesirable characters. Participatory Plant Breeding
(PPB) is the most important technology for sustainable conservation of Nepalese rice
landrace gene pool. Since, farmers were not aware on benefit sharing, and their right, P P B
will create their interest towards IPRs also.Link for e-copy: http://elibrary.narc.gov.np/?r=1132 Realities on Nepalese rice landraces [printed text] / RB Amgain ; B.K. Joshi, Author ; NP Adhikari, Author ; A Mudwari, Author ; MP Upadhayay, Author ; BK Baniya, Author . - [s.d.] . - 418-423 p.
in Rice research in Nepal : Proceedings of 24th National Summer Crops Research Workshop, Khumaltar, Lalitpur, 30-31 June 2004 / Adhikari, N.P.
Languages : English (eng)
Abstract: Rice is economically, socially, and culturally important crop of Nepal. Landraces occupies
the significant role in Nepalese rice system. They are important rice genetic resources of the
count1y. However, they are declining day by day. Household survey and focus group
discussion was carried out in Zvadul besi area (Gorkha) d!lring Nov.-Dec. 2003. Focus
group discussion iiz Kachorwa (Bara) was also conducted during Jan. 2004. The purpose of
the study was to identify the changed diversity on rice landraces, and its cause. In case of
Kachorwa, Community Biodiversity Register (CBR), 2003 was used for our purpose. Genetic
erosion was also quantified using Guarino 's score (199 5). Though, the score for genetic
erosion is not high in Zydul besi (20), the inter-varietal diversity of rice landraces was found
decreasing. Zydul besi farmers were searching for rice varieties that have higher and stable
yield, but till now no modern varieties can compete with their landraces Aanpjhutte. No. and
area un:Jer landraces was found drastically decreased in Kachorwa since last fev.• years.
Despite of higher Guarino's score (75), In-situ conservation on agro-biodiversity project is
working effectively for consen,ation of rice landraces using different approach of
conservation. However, Kachonva farmers also start the introduction of hybrid rice.
Culturally important rice landraces were found cultivated by many households in small area
only. Since, rice landraces were most 1ieed to resource poor farmers, its replacement can be
effectively reduced by removal of its undesirable characters. Participatory Plant Breeding
(PPB) is the most important technology for sustainable conservation of Nepalese rice
landrace gene pool. Since, farmers were not aware on benefit sharing, and their right, P P B
will create their interest towards IPRs also.Link for e-copy: http://elibrary.narc.gov.np/?r=1132 E-copies
http://nkcs.org.np/narc/dl/?r=1132URL
in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.
Title : Rice gene pool for mid and high hills and its conservation in Nepal Material Type: printed text Authors: B.K. Joshi, Author Pagination: 252-264 p. Languages : English (eng) Keywords: Ancestor, conservation, landrace, Nepalese rice cultivar, gene pool Abstract: Study on crop gene pool helps to develop varieties, to know the potential sources for breeding materials, to develop strategy for sustainable use and conservation and to know the richness in crop genetic resources of particular country. Individual farmers value diversity within and between their crops because of heterogeneous soils and production conditions, risk factors, market demand, consumption and uses of different products from an individual crop species. Both the potential for long term genetic gain and the reduction of genetic vulnerability may depend on the genetic diversity present in the genetic base…….
Link for e-copy: http://elibrary.narc.gov.np/?r=1154 Rice gene pool for mid and high hills and its conservation in Nepal [printed text] / B.K. Joshi, Author . - [s.d.] . - 252-264 p.
in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.
Languages : English (eng)
Keywords: Ancestor, conservation, landrace, Nepalese rice cultivar, gene pool Abstract: Study on crop gene pool helps to develop varieties, to know the potential sources for breeding materials, to develop strategy for sustainable use and conservation and to know the richness in crop genetic resources of particular country. Individual farmers value diversity within and between their crops because of heterogeneous soils and production conditions, risk factors, market demand, consumption and uses of different products from an individual crop species. Both the potential for long term genetic gain and the reduction of genetic vulnerability may depend on the genetic diversity present in the genetic base…….
Link for e-copy: http://elibrary.narc.gov.np/?r=1154 E-copies
http://nkcs.org.np/narc/dl/?r=1154URL
