|
in
| Title : |
Citrus Diseases Prevalent in Baitadi District |
| Material Type: |
printed text |
| Authors: |
C. Regmi, Author ; G. Awasthi, Author ; Bikram Thapa, Author ; Paudel, C.L., Author |
| Pagination: |
44-49 p. |
| Languages : |
English (eng) |
| Keywords: |
ELISA test, Huanglonbing, powdery mildew, PCR technique, tristeza virus |
| Abstract: |
Field survey on the prevalence of diseases was conducted during March and June 2001 in 17
commercial citrus orchards from Gajari. Bhumeswar Silang and Basuling VDCs. Laboratory
tests were made for the confirmation of Citrus Tristeza Virus (CTV) and Huanglonbing on the
samples collected from suspected trees in Royal Nepal Academy of Science and Technology's
Biotechnology Lab. The Enzyme Linked Immunosorbent Assay test was used for testing
Citrus Tristeza Virus (CTV) while Polymerase Change Reaction technique was used for
Huanglonbing. Powdery mildew was found one of the major problems especially in the
mandarin trees while anthracnose, sooty mold and greasy spot were recorded as minor
problems among the fungal diseases. The laboratory tests reveals that the frequency of CTV
(non-stem pitting strain on mandarin) is very low about 7.7 percent and that of
Huanglongbing (HLB) is about 10 percent. No vector of HLB was observed during the survey
but the several colonies of aphids - vectors of CTV were present in different orchards. |
| Link for e-copy: |
http://elibrary.narc.gov.np/?r=452 |
in
Citrus Diseases Prevalent in Baitadi District [printed text] / C. Regmi, Author ; G. Awasthi, Author ; Bikram Thapa, Author ; Paudel, C.L., Author . - [s.d.] . - 44-49 p. Languages : English ( eng) | Keywords: |
ELISA test, Huanglonbing, powdery mildew, PCR technique, tristeza virus |
| Abstract: |
Field survey on the prevalence of diseases was conducted during March and June 2001 in 17
commercial citrus orchards from Gajari. Bhumeswar Silang and Basuling VDCs. Laboratory
tests were made for the confirmation of Citrus Tristeza Virus (CTV) and Huanglonbing on the
samples collected from suspected trees in Royal Nepal Academy of Science and Technology's
Biotechnology Lab. The Enzyme Linked Immunosorbent Assay test was used for testing
Citrus Tristeza Virus (CTV) while Polymerase Change Reaction technique was used for
Huanglonbing. Powdery mildew was found one of the major problems especially in the
mandarin trees while anthracnose, sooty mold and greasy spot were recorded as minor
problems among the fungal diseases. The laboratory tests reveals that the frequency of CTV
(non-stem pitting strain on mandarin) is very low about 7.7 percent and that of
Huanglongbing (HLB) is about 10 percent. No vector of HLB was observed during the survey
but the several colonies of aphids - vectors of CTV were present in different orchards. |
| Link for e-copy: |
http://elibrary.narc.gov.np/?r=452 |
|
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