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in
| Title : |
In Vitro Plant Regeneration of Large Cardamom (Amomum subulatum Roxb.) for Virus Elimination |
| Material Type: |
printed text |
| Authors: |
Giri D.hari Subedi, Author ; Krishan Prasasd Paudyal, Author |
| Pagination: |
227-229 p. |
| Languages : |
English (eng) |
| Keywords: |
Amomum subulatum, SAP, meristemic tissue, shoot proliferation |
| Abstract: |
Laporatory experiments were carried out at National Citrus Research Program,
Dhankuta for two consecutive years {2002/2003 to 2003/2004}. in order to optimize in
vitro plant regeneration protocol of large cardamom for elimination of Chirke and f:orke
viral diseases. Meristemic tissues from the newly emerged shoots were isolated under
laminar flow hood and cultured on MS media {1962) suppl~mented with BAP tmg/litrefor
regeneration of meristem. For shoot proliferation, eight different levels of BAP were
tested in a Randomized Complete Block Design (RCBD) with four replications. The
best treatment, in terms of respons_e frequency and mean nuinber of axillary shoots per . .
f!JXplant was on MS medium {1962} supplemented with 8.0 mgllitre BAP (7.75 snootS"
per explant). Effective stem elongation was observed on MS medium. {1962)
supplemented with 1 mg/litre GA3_ where as effective rooting was observed on MS
medium (1962) supplemented with 1.0 mg/litre NAA. The in vitro rooted plantlets were
acclimatized in polyetha.ne bags inside plastic tunnel for four weeks followed by
transf()rring them into the glasshouse. The results reveled that an efficient system for in
vitro plant regeneration by multiple shoot formation from meristemic tissue of large
cardamom has been successfully established, thus it will be useful for the rapid
multiplication of disease free planting materials for commerctal plantation. |
| Link for e-copy: |
http://elibrary.narc.gov.np/?r=251 |
in
In Vitro Plant Regeneration of Large Cardamom (Amomum subulatum Roxb.) for Virus Elimination [printed text] / Giri D.hari Subedi, Author ; Krishan Prasasd Paudyal, Author . - [s.d.] . - 227-229 p. Languages : English ( eng) | Keywords: |
Amomum subulatum, SAP, meristemic tissue, shoot proliferation |
| Abstract: |
Laporatory experiments were carried out at National Citrus Research Program,
Dhankuta for two consecutive years {2002/2003 to 2003/2004}. in order to optimize in
vitro plant regeneration protocol of large cardamom for elimination of Chirke and f:orke
viral diseases. Meristemic tissues from the newly emerged shoots were isolated under
laminar flow hood and cultured on MS media {1962) suppl~mented with BAP tmg/litrefor
regeneration of meristem. For shoot proliferation, eight different levels of BAP were
tested in a Randomized Complete Block Design (RCBD) with four replications. The
best treatment, in terms of respons_e frequency and mean nuinber of axillary shoots per . .
f!JXplant was on MS medium {1962} supplemented with 8.0 mgllitre BAP (7.75 snootS"
per explant). Effective stem elongation was observed on MS medium. {1962)
supplemented with 1 mg/litre GA3_ where as effective rooting was observed on MS
medium (1962) supplemented with 1.0 mg/litre NAA. The in vitro rooted plantlets were
acclimatized in polyetha.ne bags inside plastic tunnel for four weeks followed by
transf()rring them into the glasshouse. The results reveled that an efficient system for in
vitro plant regeneration by multiple shoot formation from meristemic tissue of large
cardamom has been successfully established, thus it will be useful for the rapid
multiplication of disease free planting materials for commerctal plantation. |
| Link for e-copy: |
http://elibrary.narc.gov.np/?r=251 |
|
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