Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal / Sakha, B.M. in Nepal Agriculture Research Journal, Vol. 8 (2007)  

Public ISBD [article]  inNepal Agriculture Research Journal > Vol. 8 (2007) . - 7-13 p Title : Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal Material Type: printed text Authors: Sakha, B.M., Author ; GP Rai, Author ; Dhital, S.P., Author Publication Date: 2007 Article on page: 7-13 p Languages : English (eng) Keywords: DAS-ELISA,  meristem  excision,  potato,  pre-basic  seed,  thermotherapy Abstract: Pre-basic seed potatoes are disease free potato minitubers produced by transplanting pathogen free in vitro potato plantlets under protected condition in aphid-proof glasshouse and/or screen house. Double antibody sand witched - enzyme linked immuno-sorbant assay is used to test six major potato viruses, namely PLRV, PVS, PVX, PVY, PVA and PVM. Thermotherapy cum meristem tip excision techniques are used to eliminate these viruses. Virus free in vitro potato plantlets are rapidly propagated by single nodal cuttings on modified MS media. For pre-basic seed production disease free in vitro potato plantlets are transplanted in the sterile sand soil substrate under glasshouse and screen house, once in autumn season and next in spring season. Since 1990, National Potato Research Program has been producing about 200,000 pre-basic seeds annually. So far, PBS of 19 different recommended and released potato cultivars has been produced. Till date 3,465,799 PBS had been produced and 3,217,666 pre-basic seeds distributed to the different seed potato growers groups, District Agriculture Development Offices, government farms/research stations, and NGOs/INGOs for subsequent basic seed potato production. After establishment of tissue culture facilities in National Potato Research Program, the productivity of potato has been increased by 71% due to utilization of pre-basic seed potatoes. Link for e-copy: http://elibrary.narc.gov.np/?r=20 [article] Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal [printed text] / Sakha, B.M., Author ; GP Rai, Author ; Dhital, S.P., Author . - 2007 . - 7-13 p. Languages : English (eng) in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 7-13 p Keywords: DAS-ELISA,  meristem  excision,  potato,  pre-basic  seed,  thermotherapy Abstract: Pre-basic seed potatoes are disease free potato minitubers produced by transplanting pathogen free in vitro potato plantlets under protected condition in aphid-proof glasshouse and/or screen house. Double antibody sand witched - enzyme linked immuno-sorbant assay is used to test six major potato viruses, namely PLRV, PVS, PVX, PVY, PVA and PVM. Thermotherapy cum meristem tip excision techniques are used to eliminate these viruses. Virus free in vitro potato plantlets are rapidly propagated by single nodal cuttings on modified MS media. For pre-basic seed production disease free in vitro potato plantlets are transplanted in the sterile sand soil substrate under glasshouse and screen house, once in autumn season and next in spring season. Since 1990, National Potato Research Program has been producing about 200,000 pre-basic seeds annually. So far, PBS of 19 different recommended and released potato cultivars has been produced. Till date 3,465,799 PBS had been produced and 3,217,666 pre-basic seeds distributed to the different seed potato growers groups, District Agriculture Development Offices, government farms/research stations, and NGOs/INGOs for subsequent basic seed potato production. After establishment of tissue culture facilities in National Potato Research Program, the productivity of potato has been increased by 71% due to utilization of pre-basic seed potatoes. Link for e-copy: http://elibrary.narc.gov.np/?r=20

Relative response of potato cv deisree with N and K nutrients / Shrestha, S.L.    

Public ISBD in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.     Title : Relative response of potato cv deisree with N and K nutrients Material Type: printed text Authors: Shrestha, S.L., Author ; Khatri, B.B., Author ; GP Rai, Author ; D. Chaudhari, Author Pagination: 126-130 p. Languages : English (eng) Abstract: In order to determine and recommend the optimal dose of two major chemical fertilizers: nitrogen (N) and potassium (K20) for particular environment and particular potato variety during 1998 to 2000, a field experiment was carried out at Hattiban Research Farm, Khumaltar having low nitrogen, medium organic matter and medium potassium and high phosphorous conditions. The relative response of potato crop cv Desiree a highly nutrient responsive and widely grown potato cultivar of Nepal was evaluated with four different levels of nitrogen (0,50, 100 and  150 kg/ha) and four different levels of potassium (0, 30, 60 and 90 kg/ha)  fertilizers….. Link for e-copy: http://elibrary.narc.gov.np/?r=1066 in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.     Relative response of potato cv deisree with N and K nutrients [printed text] / Shrestha, S.L., Author ; Khatri, B.B., Author ; GP Rai, Author ; D. Chaudhari, Author . - [s.d.] . - 126-130 p. Languages : English (eng) Abstract: In order to determine and recommend the optimal dose of two major chemical fertilizers: nitrogen (N) and potassium (K20) for particular environment and particular potato variety during 1998 to 2000, a field experiment was carried out at Hattiban Research Farm, Khumaltar having low nitrogen, medium organic matter and medium potassium and high phosphorous conditions. The relative response of potato crop cv Desiree a highly nutrient responsive and widely grown potato cultivar of Nepal was evaluated with four different levels of nitrogen (0,50, 100 and  150 kg/ha) and four different levels of potassium (0, 30, 60 and 90 kg/ha)  fertilizers….. Link for e-copy: http://elibrary.narc.gov.np/?r=1066

E-copies

http://nkcs.org.np/narc/dl/?r=1066 URL