Classification of Tartary Buckwheat Accessions of West Nepal by Cluster and Principal Component Analyses / Bal K. Joshi in Nepal Agriculture Research Journal, Vol.12 (2012)  

Public ISBD [article]  inNepal Agriculture Research Journal > Vol.12 (2012) . - 22-33p. Title : Classification of Tartary Buckwheat Accessions of West Nepal by Cluster and Principal Component Analyses Material Type: printed text Authors: Bal K. Joshi, Author ; Kazutoshi, Okuno, Author Publication Date: 2012 Article on page: 22-33p. Languages : English (eng) Keywords: Fagopyrum  tataricum,  Geographical  variation,  Multivariate  analysis,  Phenotypiccharacters Link for e-copy: http://elibrary.narc.gov.np/?r=1257 [article] Classification of Tartary Buckwheat Accessions of West Nepal by Cluster and Principal Component Analyses [printed text] / Bal K. Joshi, Author ; Kazutoshi, Okuno, Author . - 2012 . - 22-33p. Languages : English (eng) in Nepal Agriculture Research Journal > Vol.12 (2012) . - 22-33p. Keywords: Fagopyrum  tataricum,  Geographical  variation,  Multivariate  analysis,  Phenotypiccharacters Link for e-copy: http://elibrary.narc.gov.np/?r=1257

Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 7 (2006)  

Public ISBD [article]  inNepal Agriculture Research Journal > Vol. 7 (2006) . - 27-36 p Title : Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum Material Type: printed text Authors: Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; Ryo Ohsawa, Author Publication Date: 2006 Article on page: 27-36 p Languages : English (eng) Keywords: Common  buckwheat,  EST  markers,  Fagopyrum  species,  transferability Abstract: If the EST primers designed for one species can be used in related species, then the cost involved in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for other species is significantly reduced. We tested the applicability of 17 EST primers developed from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18 accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones were used to design primers using Primer3. Amplification products were different in band intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight primers amplified the DNA of all accessions. Results indicated that the transferability of EST markers developed for common buckwheat decreased with an increase in genetic distance between them. Link for e-copy: http://elibrary.narc.gov.np/?r=43 [article] Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum [printed text] / Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; Ryo Ohsawa, Author . - 2006 . - 27-36 p. Languages : English (eng) in Nepal Agriculture Research Journal > Vol. 7 (2006) . - 27-36 p Keywords: Common  buckwheat,  EST  markers,  Fagopyrum  species,  transferability Abstract: If the EST primers designed for one species can be used in related species, then the cost involved in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for other species is significantly reduced. We tested the applicability of 17 EST primers developed from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18 accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones were used to design primers using Primer3. Amplification products were different in band intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight primers amplified the DNA of all accessions. Results indicated that the transferability of EST markers developed for common buckwheat decreased with an increase in genetic distance between them. Link for e-copy: http://elibrary.narc.gov.np/?r=43

Correlation and Path Coefficients Analyses of Agronomical Traits in Tartary Buckwheat / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol.10 (2010)  

Public ISBD [article]  inNepal Agriculture Research Journal > Vol.10 (2010) . - 11-22p. Title : Correlation and Path Coefficients Analyses of Agronomical Traits in Tartary Buckwheat Material Type: printed text Authors: Joshi, Bal K., Author ; Kazutoshi, Okuno, Author Publication Date: 2010 Article on page: 11-22p. Languages : English (eng) Keywords: Agronomical  traits,  Correlation,  Path  coefficient,Tartary  buckwheat Link for e-copy: http://elibrary.narc.gov.np/?r=1181 [article] Correlation and Path Coefficients Analyses of Agronomical Traits in Tartary Buckwheat [printed text] / Joshi, Bal K., Author ; Kazutoshi, Okuno, Author . - 2010 . - 11-22p. Languages : English (eng) in Nepal Agriculture Research Journal > Vol.10 (2010) . - 11-22p. Keywords: Agronomical  traits,  Correlation,  Path  coefficient,Tartary  buckwheat Link for e-copy: http://elibrary.narc.gov.np/?r=1181

Hybridization Technique in Tartary Buckwheat (Fagopyrum tataricum Gaertni) / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 9 (2009)  

Public ISBD [article]  inNepal Agriculture Research Journal > Vol. 9 (2009) . - 106-09 p Title : Hybridization Technique in Tartary Buckwheat (Fagopyrum tataricum Gaertni) Material Type: printed text Authors: Joshi, Bal K., Author ; Bimb, Hari P., Author ; Kazutoshi, Okuno, Author Publication Date: 2009 Article on page: 106-09 p General note: Research note Languages : English (eng) Link for e-copy: http://elibrary.narc.gov.np/?r=16 [article] Hybridization Technique in Tartary Buckwheat (Fagopyrum tataricum Gaertni) [printed text] / Joshi, Bal K., Author ; Bimb, Hari P., Author ; Kazutoshi, Okuno, Author . - 2009 . - 106-09 p. Research note Languages : English (eng) in Nepal Agriculture Research Journal > Vol. 9 (2009) . - 106-09 p Link for e-copy: http://elibrary.narc.gov.np/?r=16

Manual for DNA extraction in plants; technical assistance activities for genetic resources projects / Kazutoshi, Okuno

Public ISBD Title : Manual for DNA extraction in plants; technical assistance activities for genetic resources projects Material Type: printed text Authors: Kazutoshi, Okuno ; , Publisher: Tokya : JICA Pagination: 42 Keywords: Plants;  DNA;  Extraction;  Genetic  resources Manual for DNA extraction in plants; technical assistance activities for genetic resources projects [printed text] / Kazutoshi, Okuno ; , . - Tokya : JICA, [s.d.] . - 42. Keywords: Plants;  DNA;  Extraction;  Genetic  resources

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Barcode	Call number	Media type	Location	Section	Status
NR-2014	NR-2014,	Document	NARC Library	Documents	Available

Optimization of PCR Conditions for DNA Amplification of Common Buckwheat Using EST Primers / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 8 (2007)  

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