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Author Bimb, Hari P. |
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Refine your searchData Analysis Methods Adopted under In situ Global Project in Nepal / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 6 (2005)
Title : Data Analysis Methods Adopted under In situ Global Project in Nepal Material Type: printed text Authors: Joshi, Bal K., Author ; ,Upadhyay, Madhusudan, Author ; Bimb, Hari P., Author ; Gauchan, D., Author ; Baniya, B.K., Author Publication Date: 2005 Article on page: 98-110 p Languages : English (eng) Keywords: Data analysis methods, on farm research, station subject areas Abstract: Synthesizing data analysis methods adopted under in situ global project in Nepal along with
variables and nature of study could be guiding reference for researchers especially to those
involved in on farm research. The review work was conducted with the objective to help in utilizing
and managing in situ database system. The objectives of the experiment, the structure of the
treatments and the experimental design used primarily determine the type of analysis. There were 60
papers of this project published in Nepal. All these papers are grouped under 8 thematic groups
namely 1. Agroecosystem (3 papers), 2. Agromorphological and farmers’ perception (7 papers), 3.
Crop population structure (5 papers), 4. Gender, policy and general (15 papers), 5. Isozyme and
molecular (6 papers), 6. Seed systems and farmers’ networks (5 papers), 7. Social, cultural and
economical (11 papers) and 8. Value addition (8 papers). All these papers were reviewed basically
for data type, sample size, sampling methods, statistical methods and tools, varieties and
purposes. Descriptive and inferential statistics along with multivariate methods were commonly used
in on farm research. Experimental design, the most common in on station trial was least used. Study
over space and time was not adopted. There were 5 kinds of data generated, 45 statistical tools
adopted in eight different crop species. Among the 5 kinds of data under these eight subject areas,
categorical type was highest followed by discrete numerical. Binary type was least in frequency.
Most of the papers were related to rice followed by taro and finger millet. Cucumber and pigeon pea
were studied least. Descriptive statistics along with 2, multivariate analysis and regression
approaches would be appropriate tools. Similarly SPSS and MINITAB may be good software. The best
one among a number of statistical tools should be selected and utmost care must be exercised while
collecting data.Link for e-copy: http://elibrary.narc.gov.np/?r=68
in Nepal Agriculture Research Journal > Vol. 6 (2005) . - 98-110 p[article] Data Analysis Methods Adopted under In situ Global Project in Nepal [printed text] / Joshi, Bal K., Author ; ,Upadhyay, Madhusudan, Author ; Bimb, Hari P., Author ; Gauchan, D., Author ; Baniya, B.K., Author . - 2005 . - 98-110 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 6 (2005) . - 98-110 p
Keywords: Data analysis methods, on farm research, station subject areas Abstract: Synthesizing data analysis methods adopted under in situ global project in Nepal along with
variables and nature of study could be guiding reference for researchers especially to those
involved in on farm research. The review work was conducted with the objective to help in utilizing
and managing in situ database system. The objectives of the experiment, the structure of the
treatments and the experimental design used primarily determine the type of analysis. There were 60
papers of this project published in Nepal. All these papers are grouped under 8 thematic groups
namely 1. Agroecosystem (3 papers), 2. Agromorphological and farmers’ perception (7 papers), 3.
Crop population structure (5 papers), 4. Gender, policy and general (15 papers), 5. Isozyme and
molecular (6 papers), 6. Seed systems and farmers’ networks (5 papers), 7. Social, cultural and
economical (11 papers) and 8. Value addition (8 papers). All these papers were reviewed basically
for data type, sample size, sampling methods, statistical methods and tools, varieties and
purposes. Descriptive and inferential statistics along with multivariate methods were commonly used
in on farm research. Experimental design, the most common in on station trial was least used. Study
over space and time was not adopted. There were 5 kinds of data generated, 45 statistical tools
adopted in eight different crop species. Among the 5 kinds of data under these eight subject areas,
categorical type was highest followed by discrete numerical. Binary type was least in frequency.
Most of the papers were related to rice followed by taro and finger millet. Cucumber and pigeon pea
were studied least. Descriptive statistics along with 2, multivariate analysis and regression
approaches would be appropriate tools. Similarly SPSS and MINITAB may be good software. The best
one among a number of statistical tools should be selected and utmost care must be exercised while
collecting data.Link for e-copy: http://elibrary.narc.gov.np/?r=68 Genetic relationship among Nepalese rice landraces and cultivars based on rapd markers / Bimb, Hari P.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Title : Genetic relationship among Nepalese rice landraces and cultivars based on rapd markers Material Type: printed text Authors: Bimb, Hari P., Editor ; Joshi, Bal K., Author ; David Kansakar, Author ; Ekta Ghimire, Author Publisher: Hardinath, Janakpur : National Rice Research Programme Publication Date: 2010 Pagination: 168-184 p. Keywords: Genetic Distance ; rice RAPD Abstract: Genetic information of any genotype is necessary to manage and utilize them in conservation and breeding program. A total of 28
RAPD markers were used to relate the genetic structure among 50 Nepalese rice genotypes consisting of29landraces, 12 breeding
lines and 9 released cultivars. Some of them are aromatic and blast resistance. Only four primers (P41, P60, Pl09 and Pl41)
amplified the DNA of these genotypes with scorable bands. Primer 60 produced the highest number of bands (8). The highest
number of present bands (6) was shown by primer 41 in I 0 rice genotypes. Grouping of these genotypes based on the adaptation to
agro-climatic zone was not observed, probably due to low percentage coverage of genome by four primers. Most of the genotypes
grouped in two clusters. Kali Marsi and IR 24 formed separate individual cluster. Mansara and Jameli were the most similar
landraces (0.96). Churenodhan and Pranpyuri were the most closely related with Masuli. Only one genotype NR285-18 has fallen in
the first quadrant by principal component analysis and the fourth quadrant was empty. The highest contribution in PCl was from the
second band of primer 41 . This RAPD information can be used for selecting lines and for blast resistance breeding.Link for e-copy: http://elibrary.narc.gov.np/?r=1015 Genetic relationship among Nepalese rice landraces and cultivars based on rapd markers [printed text] / Bimb, Hari P., Editor ; Joshi, Bal K., Author ; David Kansakar, Author ; Ekta Ghimire, Author . - Hardinath, Janakpur : National Rice Research Programme, 2010 . - 168-184 p.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Keywords: Genetic Distance ; rice RAPD Abstract: Genetic information of any genotype is necessary to manage and utilize them in conservation and breeding program. A total of 28
RAPD markers were used to relate the genetic structure among 50 Nepalese rice genotypes consisting of29landraces, 12 breeding
lines and 9 released cultivars. Some of them are aromatic and blast resistance. Only four primers (P41, P60, Pl09 and Pl41)
amplified the DNA of these genotypes with scorable bands. Primer 60 produced the highest number of bands (8). The highest
number of present bands (6) was shown by primer 41 in I 0 rice genotypes. Grouping of these genotypes based on the adaptation to
agro-climatic zone was not observed, probably due to low percentage coverage of genome by four primers. Most of the genotypes
grouped in two clusters. Kali Marsi and IR 24 formed separate individual cluster. Mansara and Jameli were the most similar
landraces (0.96). Churenodhan and Pranpyuri were the most closely related with Masuli. Only one genotype NR285-18 has fallen in
the first quadrant by principal component analysis and the fourth quadrant was empty. The highest contribution in PCl was from the
second band of primer 41 . This RAPD information can be used for selecting lines and for blast resistance breeding.Link for e-copy: http://elibrary.narc.gov.np/?r=1015 E-copies
http://nkcs.org.np/narc/dl/?r=1015URL
Title : Hybridization Technique in Tartary Buckwheat (Fagopyrum tataricum Gaertni) Material Type: printed text Authors: Joshi, Bal K., Author ; Bimb, Hari P., Author ; Kazutoshi, Okuno, Author Publication Date: 2009 Article on page: 106-09 p General note: Research note Languages : English (eng) Link for e-copy: http://elibrary.narc.gov.np/?r=16
in Nepal Agriculture Research Journal > Vol. 9 (2009) . - 106-09 p[article] Hybridization Technique in Tartary Buckwheat (Fagopyrum tataricum Gaertni) [printed text] / Joshi, Bal K., Author ; Bimb, Hari P., Author ; Kazutoshi, Okuno, Author . - 2009 . - 106-09 p.
Research note
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 9 (2009) . - 106-09 p
Link for e-copy: http://elibrary.narc.gov.np/?r=16
Title : Isozyme Variations in Fine and Aromatic Rice Genotypes Material Type: printed text Authors: Bimb, Hari P., Author ; Sah, Ram P., Author ; Navin L. Karn, Author Publication Date: 2004 Article on page: 59-64 p Languages : English (eng) Keywords: Fine and aromatic rice, genetic diversity, isozyme Abstract: Allozyme electrophoresis has been a reliable technique in estimating the genetic variation in
crops.
We surveyed isozyme variation of 24 aromatic and fine rice varieties collected from Nepal including
Jetho budho landrace. Five enzyme systems were analyzed for allozyme variability that detected
distinct 10 isozyme loci and 15 alleles including null alleles. The polymorphic enzyme
systems, esterase revealed 8 phenotypes, 6- phosphoglutanate dehydrogenase and peroxidase each
with 3 phenotypes and alcohol dehydrogenase and aspartate aminotransferase each with 2 phenotypes
based on the combination of presence and absence of alleles. Dendrogram did not indicate any clear
pattern of these populations into definite clusters. However 3 groups were detected at 84% of
dissimilarity among 24 populations. Nepalese varieties Rato Basmati, Pahenle and Jetho Budho
grouped in a separate cluster in the dendrogram.Link for e-copy: http://elibrary.narc.gov.np/?r=80
in Nepal Agriculture Research Journal > Vol. 5 (2004) . - 59-64 p[article] Isozyme Variations in Fine and Aromatic Rice Genotypes [printed text] / Bimb, Hari P., Author ; Sah, Ram P., Author ; Navin L. Karn, Author . - 2004 . - 59-64 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 5 (2004) . - 59-64 p
Keywords: Fine and aromatic rice, genetic diversity, isozyme Abstract: Allozyme electrophoresis has been a reliable technique in estimating the genetic variation in
crops.
We surveyed isozyme variation of 24 aromatic and fine rice varieties collected from Nepal including
Jetho budho landrace. Five enzyme systems were analyzed for allozyme variability that detected
distinct 10 isozyme loci and 15 alleles including null alleles. The polymorphic enzyme
systems, esterase revealed 8 phenotypes, 6- phosphoglutanate dehydrogenase and peroxidase each
with 3 phenotypes and alcohol dehydrogenase and aspartate aminotransferase each with 2 phenotypes
based on the combination of presence and absence of alleles. Dendrogram did not indicate any clear
pattern of these populations into definite clusters. However 3 groups were detected at 84% of
dissimilarity among 24 populations. Nepalese varieties Rato Basmati, Pahenle and Jetho Budho
grouped in a separate cluster in the dendrogram.Link for e-copy: http://elibrary.narc.gov.np/?r=80
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Title : Molecular Tagging, Allele mining and marker aided breeding for blast resistance in rice Material Type: printed text Authors: Joshi, Bal K., Editor ; Bimb, Hari P., Author ; Gopal Parajuli, Author ; Bedanand Chaudhary, Author Publication Date: 2010 Pagination: 189-202 p. Keywords: Blast resistance ; DNA marker Gene tagging rice Abstract: Breeding work utilizing both genotypic and phenotypic· markers is the most effective way of achieving target. Molecular markers eg
restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and simi'~~ sequence repeat (SSR) tightly
linked to target gene have been identified in different chromosomes to impose the genetic selection ie marker assisted selection
(MAS). This paper summarize the progress and achievement made in breeding for blast resistance based on DNA markers which
help in planning blast resistance gene tagging in Nepalese rice genotypes and developing blast resistant inbred line or near isogenic
line (NIL). Blast fungus (Pyricularia oryzae Cav.) can infect plants at any growth stage from seedling to maturity and at any part eg
leaf, node, internode, neck and seed. Qualitative resistance gene may occasionally be broken down due to numerous races of blast
fungus both physiological and geographical races available in Nepal. Quantitative gene resistance and gene pyramiding are the best
alternative for creating durable resistance system. At least 40 genes conferring resistance to blast isolates with multiple alleles have
been described. Both dominant and recessive resistance alleles have been found in many rice landraces. Morphological and isozymic
markers are limited in number. Therefore, highly polymorphic and easily detectable SSR markers are being used in breeding for
blast resistance. Bulked segregant analysis (BSA) is the simple method for tagging resistance ge~e by SSR markers. Quantitative
trait loci (QTLs) have also been mapped and· most of them are linked to qualitative genes. SSR markers linked to the gene are being
used to select plants possessing the desired trait and markers throughout the genome are being used to select plants that are
genetically similar to recurrent parent. Using SSR markers it may be possible to select blast resistance genotypes at any stage of crop
development from any small part of crop, to conduct many round of selection, to select without inoculums, without scoring, and
without testing in hot spot or artificial inoculation. Molecular based blast resistance breeding work is initiated focusing on resistance
gene tagging in Nepalese rice Iandraces and transferring resistance genes in cvs Jumli Marshi, K.humal-4 and Mansuli.Link for e-copy: http://elibrary.narc.gov.np/?r=1016 Molecular Tagging, Allele mining and marker aided breeding for blast resistance in rice [printed text] / Joshi, Bal K., Editor ; Bimb, Hari P., Author ; Gopal Parajuli, Author ; Bedanand Chaudhary, Author . - 2010 . - 189-202 p.
in Summer crops research in Nepal: Proceedings of the 25th Summer Crops Workshop held at Kathmandu 21-23 June 2007 / Adhikari, N.P.
Keywords: Blast resistance ; DNA marker Gene tagging rice Abstract: Breeding work utilizing both genotypic and phenotypic· markers is the most effective way of achieving target. Molecular markers eg
restriction fragment length polymorphism (RFLP), single nucleotide polymorphism (SNP) and simi'~~ sequence repeat (SSR) tightly
linked to target gene have been identified in different chromosomes to impose the genetic selection ie marker assisted selection
(MAS). This paper summarize the progress and achievement made in breeding for blast resistance based on DNA markers which
help in planning blast resistance gene tagging in Nepalese rice genotypes and developing blast resistant inbred line or near isogenic
line (NIL). Blast fungus (Pyricularia oryzae Cav.) can infect plants at any growth stage from seedling to maturity and at any part eg
leaf, node, internode, neck and seed. Qualitative resistance gene may occasionally be broken down due to numerous races of blast
fungus both physiological and geographical races available in Nepal. Quantitative gene resistance and gene pyramiding are the best
alternative for creating durable resistance system. At least 40 genes conferring resistance to blast isolates with multiple alleles have
been described. Both dominant and recessive resistance alleles have been found in many rice landraces. Morphological and isozymic
markers are limited in number. Therefore, highly polymorphic and easily detectable SSR markers are being used in breeding for
blast resistance. Bulked segregant analysis (BSA) is the simple method for tagging resistance ge~e by SSR markers. Quantitative
trait loci (QTLs) have also been mapped and· most of them are linked to qualitative genes. SSR markers linked to the gene are being
used to select plants possessing the desired trait and markers throughout the genome are being used to select plants that are
genetically similar to recurrent parent. Using SSR markers it may be possible to select blast resistance genotypes at any stage of crop
development from any small part of crop, to conduct many round of selection, to select without inoculums, without scoring, and
without testing in hot spot or artificial inoculation. Molecular based blast resistance breeding work is initiated focusing on resistance
gene tagging in Nepalese rice Iandraces and transferring resistance genes in cvs Jumli Marshi, K.humal-4 and Mansuli.Link for e-copy: http://elibrary.narc.gov.np/?r=1016 E-copies
http://nkcs.org.np/narc/dl/?r=1016URL
