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Author Sakha, B.M. |
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Refine your searchDisease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal / Sakha, B.M. in Nepal Agriculture Research Journal, Vol. 8 (2007)
Title : Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal Material Type: printed text Authors: Sakha, B.M., Author ; GP Rai, Author ; Dhital, S.P., Author Publication Date: 2007 Article on page: 7-13 p Languages : English (eng) Keywords: DAS-ELISA, meristem excision, potato, pre-basic seed, thermotherapy Abstract: Pre-basic seed potatoes are disease free potato minitubers produced by transplanting
pathogen free in vitro potato plantlets under protected condition in aphid-proof glasshouse
and/or screen house. Double antibody sand witched - enzyme linked immuno-sorbant assay
is used to test six major potato viruses, namely PLRV, PVS, PVX, PVY, PVA and PVM.
Thermotherapy cum meristem tip excision techniques are used to eliminate these viruses.
Virus free in vitro potato plantlets are rapidly propagated by single nodal cuttings on
modified MS media. For pre-basic seed production disease free in vitro potato plantlets are
transplanted in the sterile sand soil substrate under glasshouse and screen house, once in
autumn season and next in spring season. Since 1990, National Potato Research Program has
been producing about 200,000 pre-basic seeds annually. So far, PBS of 19 different
recommended and released potato cultivars has been produced. Till date 3,465,799 PBS had
been produced and 3,217,666 pre-basic seeds distributed to the different seed potato growers
groups, District Agriculture Development Offices, government farms/research stations, and
NGOs/INGOs for subsequent basic seed potato production. After establishment of tissue
culture facilities in National Potato Research Program, the productivity of potato has been
increased by 71% due to utilization of pre-basic seed potatoes.Link for e-copy: http://elibrary.narc.gov.np/?r=20
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 7-13 p[article] Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal [printed text] / Sakha, B.M., Author ; GP Rai, Author ; Dhital, S.P., Author . - 2007 . - 7-13 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 7-13 p
Keywords: DAS-ELISA, meristem excision, potato, pre-basic seed, thermotherapy Abstract: Pre-basic seed potatoes are disease free potato minitubers produced by transplanting
pathogen free in vitro potato plantlets under protected condition in aphid-proof glasshouse
and/or screen house. Double antibody sand witched - enzyme linked immuno-sorbant assay
is used to test six major potato viruses, namely PLRV, PVS, PVX, PVY, PVA and PVM.
Thermotherapy cum meristem tip excision techniques are used to eliminate these viruses.
Virus free in vitro potato plantlets are rapidly propagated by single nodal cuttings on
modified MS media. For pre-basic seed production disease free in vitro potato plantlets are
transplanted in the sterile sand soil substrate under glasshouse and screen house, once in
autumn season and next in spring season. Since 1990, National Potato Research Program has
been producing about 200,000 pre-basic seeds annually. So far, PBS of 19 different
recommended and released potato cultivars has been produced. Till date 3,465,799 PBS had
been produced and 3,217,666 pre-basic seeds distributed to the different seed potato growers
groups, District Agriculture Development Offices, government farms/research stations, and
NGOs/INGOs for subsequent basic seed potato production. After establishment of tissue
culture facilities in National Potato Research Program, the productivity of potato has been
increased by 71% due to utilization of pre-basic seed potatoes.Link for e-copy: http://elibrary.narc.gov.np/?r=20
Title : In Vitro Shoot Propagation And Micrituberization In Potato(Solanum Tuberosum L.) Material Type: printed text Authors: Sakha, B.M., Author Publisher: Hisar : Chaudhary Charan Singh Haryana Agricultural University Publication Date: 2001 Pagination: 59 p. Languages : English (eng) Keywords: introduction;review of literature;materials and methods;results;discussion;summary ans conclusion Link for e-copy: http://elibrary.narc.gov.np/?r=3682 In Vitro Shoot Propagation And Micrituberization In Potato(Solanum Tuberosum L.) [printed text] / Sakha, B.M., Author . - Hisar : Chaudhary Charan Singh Haryana Agricultural University, 2001 . - 59 p.
Languages : English (eng)
Keywords: introduction;review of literature;materials and methods;results;discussion;summary ans conclusion Link for e-copy: http://elibrary.narc.gov.np/?r=3682 Copies (1)
Barcode Call number Media type Location Section Status 5123 SAK Document NARC Library Documents Available
Title : In vitro shoot propagation and microtuberization in potato (Solanum tubersum l.) Material Type: printed text Authors: Sakha, B.M. ; , Publisher: Hisar : Chaudhary Charan Singh Haryana Agricultural University Publication Date: 2001 Pagination: 59 General note: AN: NR-5123 Keywords: Potatoes; Root vegetables; Plant propagation; Vegetables Class number: 635.21 In vitro shoot propagation and microtuberization in potato (Solanum tubersum l.) [printed text] / Sakha, B.M. ; , . - Hisar : Chaudhary Charan Singh Haryana Agricultural University, 2001 . - 59.
AN: NR-5123
Keywords: Potatoes; Root vegetables; Plant propagation; Vegetables Class number: 635.21 Copies (1)
Barcode Call number Media type Location Section Status NARC2005123 635.21, SAK Document NARC Library Documents Available
Title : IPY-8 and Khumal Laxmi: Newly Released Potato Varieties Material Type: printed text Authors: Khatri, B.B., Author ; Luitel, B.P., Author ; D. Chaudhary, Author ; Sharma, B.P., Author ; Ghimire, J., Author ; Sakha, B.M., Author ; Dhital, S.P., Author ; G. P. Rai, Author Publication Date: 2010 Article on page: 30-38 p. Languages : English (eng)
in Nepalese Horticulture > v.7 [07/25/2010] . - 30-38 p.[article] IPY-8 and Khumal Laxmi: Newly Released Potato Varieties [printed text] / Khatri, B.B., Author ; Luitel, B.P., Author ; D. Chaudhary, Author ; Sharma, B.P., Author ; Ghimire, J., Author ; Sakha, B.M., Author ; Dhital, S.P., Author ; G. P. Rai, Author . - 2010 . - 30-38 p.
Languages : English (eng)
in Nepalese Horticulture > v.7 [07/25/2010] . - 30-38 p.Performance of In-Vitro Plantlets and Stem Cuttings for Pre-Basic Seed Potato Production Under Glasshouse / Sakha, B.M. ; G. P. Rai
Title : Performance of In-Vitro Plantlets and Stem Cuttings for Pre-Basic Seed Potato Production Under Glasshouse Material Type: printed text Authors: Sakha, B.M., Author ; G. P. Rai, Author Pagination: 186-189 p. Languages : English (eng) Keywords: cardinal. In vitro, mini tubers, PBS, plant geometry, stem cuttings Abstract: Four weeks old in t;itro plantlets and stem cuttings of potato cv. Cardinal were plallted under
glasshouse with three plallf geomett)'· viz. 1 OxJO em. 1 5x I 5 em and 20x I 0 em to study the
petformance of theie plallfing materials for pre-basic seed (PBS) production. The study was
conducted in RCBD replicated thrice and the data analyzed with MSTAT-C. It was fotmd that
in vitro plantlers produced five minitttbers per plant as compared to two from stem cuttings.
However, the mean tuber size of PBS produced from stem cuttings was 8. 7 as against 2.8 g
produced from in vitro plantlets. The plantlets planted with 10x10 em spacing yielded the
highest number (294/nl) of PBS. Hence 10 x 10 em spacing is recommended for in vitro
plantlets to maximize the mm1ber of PBS yield and for stem cuttings to maximize minituber
yield as well as minimize the mean minituber size.Link for e-copy: http://elibrary.narc.gov.np/?r=488 Performance of In-Vitro Plantlets and Stem Cuttings for Pre-Basic Seed Potato Production Under Glasshouse [printed text] / Sakha, B.M., Author ; G. P. Rai, Author . - [s.d.] . - 186-189 p.
Languages : English (eng)
Keywords: cardinal. In vitro, mini tubers, PBS, plant geometry, stem cuttings Abstract: Four weeks old in t;itro plantlets and stem cuttings of potato cv. Cardinal were plallted under
glasshouse with three plallf geomett)'· viz. 1 OxJO em. 1 5x I 5 em and 20x I 0 em to study the
petformance of theie plallfing materials for pre-basic seed (PBS) production. The study was
conducted in RCBD replicated thrice and the data analyzed with MSTAT-C. It was fotmd that
in vitro plantlers produced five minitttbers per plant as compared to two from stem cuttings.
However, the mean tuber size of PBS produced from stem cuttings was 8. 7 as against 2.8 g
produced from in vitro plantlets. The plantlets planted with 10x10 em spacing yielded the
highest number (294/nl) of PBS. Hence 10 x 10 em spacing is recommended for in vitro
plantlets to maximize the mm1ber of PBS yield and for stem cuttings to maximize minituber
yield as well as minimize the mean minituber size.Link for e-copy: http://elibrary.narc.gov.np/?r=488 E-copies
http://nkcs.org.np/narc/dl/?r=488URL
