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Characteristics of Ralstonia Solanacearum Strains of Potato Wilt Disease from Nepal and Thailand / Dhital, S.P. in Nepal Agriculture Research Journal, Vol. 4 (2000/2001)
[article]
Title : Characteristics of Ralstonia Solanacearum Strains of Potato Wilt Disease from Nepal and Thailand Material Type: printed text Authors: Dhital, S.P., Author ; N. Thaveechai, Author ; Sundar K. Shrestha, Author Publication Date: 2000 Article on page: 42-47 p Languages : English (eng) Keywords: Key words: Bacterial wilt, potato, Pseudomonas solanacearum, Ralstonia solanacearum Abstract: Characterization of strains of Ralstonia solanacearum, the causal agent of potato bacterial wilt
disease from Nepal and Thailand was performed based on pathogenicity,
biochemical/physiological and serological tests. Fifteen R. solanacearum strains isolated from wilt
infected potato plants and tubers grown in Nepal were characterized as race 3, biovar II based on
the pathogenicity on different host plants, hypersensitive reaction on tobacco leaf and utilization of
some sugars. Results of pathogenicity test show that all strains from Nepal had limited host range.
Degree of virulence of all strains varied from high to medium in potato and tomato and medium to
low in eggplant. They did not cause wilting in tobacco, pepper and peanut plants. Six strains from
Thailand were characterized as biovar II and III. Additionally, comparisons on the physiological,
biological and serological characters of seven strains from Nepal and six from Thailand revealed
similar characters. Race 3 and biovar II of the pathogen was widely spread over potato growing
areas of mid and high hills of Nepal. Both biovars II and III were prevalent in the potato growing
areas of Thailand but biovar III was the most dominating one.Link for e-copy: http://elibrary.narc.gov.np/?r=92
in Nepal Agriculture Research Journal > Vol. 4 (2000/2001) . - 42-47 p[article] Characteristics of Ralstonia Solanacearum Strains of Potato Wilt Disease from Nepal and Thailand [printed text] / Dhital, S.P., Author ; N. Thaveechai, Author ; Sundar K. Shrestha, Author . - 2000 . - 42-47 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 4 (2000/2001) . - 42-47 p
Keywords: Key words: Bacterial wilt, potato, Pseudomonas solanacearum, Ralstonia solanacearum Abstract: Characterization of strains of Ralstonia solanacearum, the causal agent of potato bacterial wilt
disease from Nepal and Thailand was performed based on pathogenicity,
biochemical/physiological and serological tests. Fifteen R. solanacearum strains isolated from wilt
infected potato plants and tubers grown in Nepal were characterized as race 3, biovar II based on
the pathogenicity on different host plants, hypersensitive reaction on tobacco leaf and utilization of
some sugars. Results of pathogenicity test show that all strains from Nepal had limited host range.
Degree of virulence of all strains varied from high to medium in potato and tomato and medium to
low in eggplant. They did not cause wilting in tobacco, pepper and peanut plants. Six strains from
Thailand were characterized as biovar II and III. Additionally, comparisons on the physiological,
biological and serological characters of seven strains from Nepal and six from Thailand revealed
similar characters. Race 3 and biovar II of the pathogen was widely spread over potato growing
areas of mid and high hills of Nepal. Both biovars II and III were prevalent in the potato growing
areas of Thailand but biovar III was the most dominating one.Link for e-copy: http://elibrary.narc.gov.np/?r=92 Participatory Evaluation of Some Tomato Genotypes for Resistance to Bacterial Wilt / Timila (Dhawa), R.D. in Nepal Agriculture Research Journal, Vol. 8 (2007)
[article]
Title : Participatory Evaluation of Some Tomato Genotypes for Resistance to Bacterial Wilt Material Type: printed text Authors: Timila (Dhawa), R.D., Author ; Sharada Joshi, Author Publication Date: 2007 Article on page: 50-55 p Languages : English (eng) Keywords: Bacterial wilt, Ralstonia solanacearum, resistant variety, Tomato Abstract: Bacterial wilt caused by Ralstonia solanacearum E.F. Smith is one of the destructive
diseases of tomato. Experiments were conducted to confirm the resistance and to evaluate
varieties received from Asian Vegetable Research and Development Center against bacterial
wilt disease in the farmers’ bacterial wilt sick fields at Thaiba and at Panchkhal during 1999
and 2000-2001 respectively. A total of five varieties such as CLN 2026 C, CLN 2026 D,
CLN 1466 J, CLN 1466 P and susceptible check Lapsigede or L 390 were included in the
experiments. Randomized complete block design with 4 replications was commenced at
both the locations. In terms of disease incidence and yield parameters as well as consumers’
preference on the fruit size, CLN 2026 C was found the best variety followed by CLN 2026
D. Wilt incidence was significantly lower in those varieties showing resistant reaction than
in susceptible check. However, the marketable fruit yields were significantly higher with
low wilt incidences in CLN 1466 P and CLN 1466 J also compared to susceptible check.
But the large fruit size of those varieties was not acceptable to the consumers and the
growers. Thus, CLN 2026 C and CLN 2026 D could be used as resistant variety to minimize
crop losses from bacterial wilt disease.Link for e-copy: http://elibrary.narc.gov.np/?r=27
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 50-55 p[article] Participatory Evaluation of Some Tomato Genotypes for Resistance to Bacterial Wilt [printed text] / Timila (Dhawa), R.D., Author ; Sharada Joshi, Author . - 2007 . - 50-55 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 50-55 p
Keywords: Bacterial wilt, Ralstonia solanacearum, resistant variety, Tomato Abstract: Bacterial wilt caused by Ralstonia solanacearum E.F. Smith is one of the destructive
diseases of tomato. Experiments were conducted to confirm the resistance and to evaluate
varieties received from Asian Vegetable Research and Development Center against bacterial
wilt disease in the farmers’ bacterial wilt sick fields at Thaiba and at Panchkhal during 1999
and 2000-2001 respectively. A total of five varieties such as CLN 2026 C, CLN 2026 D,
CLN 1466 J, CLN 1466 P and susceptible check Lapsigede or L 390 were included in the
experiments. Randomized complete block design with 4 replications was commenced at
both the locations. In terms of disease incidence and yield parameters as well as consumers’
preference on the fruit size, CLN 2026 C was found the best variety followed by CLN 2026
D. Wilt incidence was significantly lower in those varieties showing resistant reaction than
in susceptible check. However, the marketable fruit yields were significantly higher with
low wilt incidences in CLN 1466 P and CLN 1466 J also compared to susceptible check.
But the large fruit size of those varieties was not acceptable to the consumers and the
growers. Thus, CLN 2026 C and CLN 2026 D could be used as resistant variety to minimize
crop losses from bacterial wilt disease.Link for e-copy: http://elibrary.narc.gov.np/?r=27 Biological Control of Fungal Diseases of Potato / Bijaya Laxmi Maharjan ; Shaila Basnyat ; Kanti Shrestha
in Advancing horticultural research for development. Proceedings of the 7th National Horticulture Seminar , Khumaltar, Lalitpur, 12-14 June 2011 (Jestha 29-31, 2068) / Paudyal, K.P.
Title : Biological Control of Fungal Diseases of Potato Material Type: printed text Authors: Bijaya Laxmi Maharjan, Author ; Shaila Basnyat, Author ; Kanti Shrestha, Author Pagination: 126-129 p. Languages : English (eng) Keywords: late blight. early blight, fusaria/ wilt, plant extract Abstract: Solanum tuberosum (Potato) is one of the most important tuber crops with the production of 315
million tons worldwide per year. In the present study an attempt has been made to screen and
· evaluate the antifungal activity of plant extracts against the fungal pathogens of potato plant.
Crude ethanolic extracts of five different plant materials viz. Brassica nigra (cake), Cinnamomum
camphora (fruits), Eupatorium adenophorum (twigs), Lantana camara (twigs) and Melia
azedarach (fruits) were screened and tested against the three fungal isolates (Phytophthora
infestans, Alternaria so/ani and Fusarium oxysporum) from diseased potato leaf samples. The
antifungal activity of the crude extracts obtained was evaluated by agar well diffusion method and
two fold broth dilution method. C. camphora gave the highest yield of700/6 while M azedarach had
the lowest yield of 9. 7 5% of crude extracts. B. nigra was found most effective against P. infestans
with both MIC and MFC values 6.15mg/ml. Similarly, M azedarach showed the higher antifungal
activity against A. so/ani with both MIC and MFC values 3.115mglml. C. camphora was found
least effective against P. infestans and A. so/ani while it was most effective against F. oxysporum
with both MIC and MFC values 3.115 mg/ml. Different types of plant extracts with different
concentration significantly inhibited the growth of all the fungal pathogens at P (<0.05). The
extracts used in this experiment were found to be suitable for the control of these fungal pathogens.Link for e-copy: http://elibrary.narc.gov.np/?r=1653 Biological Control of Fungal Diseases of Potato [printed text] / Bijaya Laxmi Maharjan, Author ; Shaila Basnyat, Author ; Kanti Shrestha, Author . - [s.d.] . - 126-129 p.
in Advancing horticultural research for development. Proceedings of the 7th National Horticulture Seminar , Khumaltar, Lalitpur, 12-14 June 2011 (Jestha 29-31, 2068) / Paudyal, K.P.
Languages : English (eng)
Keywords: late blight. early blight, fusaria/ wilt, plant extract Abstract: Solanum tuberosum (Potato) is one of the most important tuber crops with the production of 315
million tons worldwide per year. In the present study an attempt has been made to screen and
· evaluate the antifungal activity of plant extracts against the fungal pathogens of potato plant.
Crude ethanolic extracts of five different plant materials viz. Brassica nigra (cake), Cinnamomum
camphora (fruits), Eupatorium adenophorum (twigs), Lantana camara (twigs) and Melia
azedarach (fruits) were screened and tested against the three fungal isolates (Phytophthora
infestans, Alternaria so/ani and Fusarium oxysporum) from diseased potato leaf samples. The
antifungal activity of the crude extracts obtained was evaluated by agar well diffusion method and
two fold broth dilution method. C. camphora gave the highest yield of700/6 while M azedarach had
the lowest yield of 9. 7 5% of crude extracts. B. nigra was found most effective against P. infestans
with both MIC and MFC values 6.15mg/ml. Similarly, M azedarach showed the higher antifungal
activity against A. so/ani with both MIC and MFC values 3.115mglml. C. camphora was found
least effective against P. infestans and A. so/ani while it was most effective against F. oxysporum
with both MIC and MFC values 3.115 mg/ml. Different types of plant extracts with different
concentration significantly inhibited the growth of all the fungal pathogens at P (<0.05). The
extracts used in this experiment were found to be suitable for the control of these fungal pathogens.Link for e-copy: http://elibrary.narc.gov.np/?r=1653 E-copies
http://nkcs.org.np/narc/dl/?r=1653URL