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Title : Citrus Diseases Prevalent in Baitadi District Material Type: printed text Authors: C. Regmi, Author ; G. Awasthi, Author ; Bikram Thapa, Author ; Paudel, C.L., Author Pagination: 44-49 p. Languages : English (eng) Keywords: ELISA test, Huanglonbing, powdery mildew, PCR technique, tristeza virus Abstract: Field survey on the prevalence of diseases was conducted during March and June 2001 in 17
commercial citrus orchards from Gajari. Bhumeswar Silang and Basuling VDCs. Laboratory
tests were made for the confirmation of Citrus Tristeza Virus (CTV) and Huanglonbing on the
samples collected from suspected trees in Royal Nepal Academy of Science and Technology's
Biotechnology Lab. The Enzyme Linked Immunosorbent Assay test was used for testing
Citrus Tristeza Virus (CTV) while Polymerase Change Reaction technique was used for
Huanglonbing. Powdery mildew was found one of the major problems especially in the
mandarin trees while anthracnose, sooty mold and greasy spot were recorded as minor
problems among the fungal diseases. The laboratory tests reveals that the frequency of CTV
(non-stem pitting strain on mandarin) is very low about 7.7 percent and that of
Huanglongbing (HLB) is about 10 percent. No vector of HLB was observed during the survey
but the several colonies of aphids - vectors of CTV were present in different orchards.Link for e-copy: http://elibrary.narc.gov.np/?r=452 Citrus Diseases Prevalent in Baitadi District [printed text] / C. Regmi, Author ; G. Awasthi, Author ; Bikram Thapa, Author ; Paudel, C.L., Author . - [s.d.] . - 44-49 p.
Languages : English (eng)
Keywords: ELISA test, Huanglonbing, powdery mildew, PCR technique, tristeza virus Abstract: Field survey on the prevalence of diseases was conducted during March and June 2001 in 17
commercial citrus orchards from Gajari. Bhumeswar Silang and Basuling VDCs. Laboratory
tests were made for the confirmation of Citrus Tristeza Virus (CTV) and Huanglonbing on the
samples collected from suspected trees in Royal Nepal Academy of Science and Technology's
Biotechnology Lab. The Enzyme Linked Immunosorbent Assay test was used for testing
Citrus Tristeza Virus (CTV) while Polymerase Change Reaction technique was used for
Huanglonbing. Powdery mildew was found one of the major problems especially in the
mandarin trees while anthracnose, sooty mold and greasy spot were recorded as minor
problems among the fungal diseases. The laboratory tests reveals that the frequency of CTV
(non-stem pitting strain on mandarin) is very low about 7.7 percent and that of
Huanglongbing (HLB) is about 10 percent. No vector of HLB was observed during the survey
but the several colonies of aphids - vectors of CTV were present in different orchards.Link for e-copy: http://elibrary.narc.gov.np/?r=452 E-copies
http://nkcs.org.np/narc/dl/?r=452URL
in Agricultural research for poverty alleviation and livelihood enhancement. Proceedings of the 3rd SAS-N Convention 27-29 August 2008, Lalitpur / Paudyal, Krishna Prasad
Title : Overview of bovine paratuberculosis in Nepal Material Type: printed text Authors: U. M. Singh, Author ; Pradhan, A., Author ; T.R Gumpo, Author ; S Sivakoti, Author ; S Sah, Author Pagination: 302-305 p. Languages : English (eng) Keywords: Agar gel immunodiffuson test, ELISA, infectious disease, Paratuberculosis, prevalence of the disease Abstract: An attempt was made to know the status of Paratuberculosis (Johne’s disease) a chronic granulomatous infectious disease of ruminants in Nepal. In past years intradermal skin test (Johnin test) had been conducted to identify the prevalence of the disease. Intradermal skin test carried out during 1982- 1988 in cattle of organized farms of Kathmandu and Pokhara valley showed an over all prevalence of 3.22 percent (0.9-10.13%).In 1999,115 serum samples from buffaloes and 98 serum samples from cattle of different districts of Western Nepal revealed 39 samples (33.9%) and 27(27.5%) samples positive to antibodies against paratuberculosis by absorbed ELISA……………….
Link for e-copy: http://elibrary.narc.gov.np/?r=1539 Overview of bovine paratuberculosis in Nepal [printed text] / U. M. Singh, Author ; Pradhan, A., Author ; T.R Gumpo, Author ; S Sivakoti, Author ; S Sah, Author . - [s.d.] . - 302-305 p.
in Agricultural research for poverty alleviation and livelihood enhancement. Proceedings of the 3rd SAS-N Convention 27-29 August 2008, Lalitpur / Paudyal, Krishna Prasad
Languages : English (eng)
Keywords: Agar gel immunodiffuson test, ELISA, infectious disease, Paratuberculosis, prevalence of the disease Abstract: An attempt was made to know the status of Paratuberculosis (Johne’s disease) a chronic granulomatous infectious disease of ruminants in Nepal. In past years intradermal skin test (Johnin test) had been conducted to identify the prevalence of the disease. Intradermal skin test carried out during 1982- 1988 in cattle of organized farms of Kathmandu and Pokhara valley showed an over all prevalence of 3.22 percent (0.9-10.13%).In 1999,115 serum samples from buffaloes and 98 serum samples from cattle of different districts of Western Nepal revealed 39 samples (33.9%) and 27(27.5%) samples positive to antibodies against paratuberculosis by absorbed ELISA……………….
Link for e-copy: http://elibrary.narc.gov.np/?r=1539 E-copies
http://nkcs.org.np/narc/dl/?r=1539URL Storage Behavior of Large Cardamom (Ammomum subulatum Roxb.) Seed / Purushottam P. Khatiwada ; Sharmila Piya ; Neelam Subba
in Proceedings of the fifth National Seminar on Horticulture June 9-10, 2008 / Nepal Agricultural Research Council (NARC) (Khumaltar, Lalitpur, Nepal)
Title : Storage Behavior of Large Cardamom (Ammomum subulatum Roxb.) Seed Material Type: printed text Authors: Purushottam P. Khatiwada, Author ; Sharmila Piya, Author ; Neelam Subba, Author Pagination: 230-233 p. Languages : English (eng) Keywords: large cardamom, storage conditions, seed, tetrazolium test, viability Abstract: Poor and irregular germiniition of large cardamom seed become a problem of
nursery growers and recalcitrant nature and dormancy are believed to be the
factors responsible with it. An experiment was carried for three consecutive years
to identify the seed viability period stored in different conditions with different seed
moisture level using tetrazolium test. The ·results showed that the seed remain
viable (>80%) even dried to less than 11% moisture level in electric oven. There
was non-significant difference in seed viability stored under refrigerator and
ambient condition till 150 days after seed drying by both methods. However, effect
of storage duration was prominent (p>.001) where viability was not statistically
different between 15 and 60 days after drying. The result has clearly proved that
large cardamom seed has orthodox behavior of storage despite farmers' notion of
recalcitrant nature of the seed. In addition to th(s finding, light has been shed to the
other basic information of large cardamom seed.Link for e-copy: http://elibrary.narc.gov.np/?r=252 Storage Behavior of Large Cardamom (Ammomum subulatum Roxb.) Seed [printed text] / Purushottam P. Khatiwada, Author ; Sharmila Piya, Author ; Neelam Subba, Author . - [s.d.] . - 230-233 p.
in Proceedings of the fifth National Seminar on Horticulture June 9-10, 2008 / Nepal Agricultural Research Council (NARC) (Khumaltar, Lalitpur, Nepal)
Languages : English (eng)
Keywords: large cardamom, storage conditions, seed, tetrazolium test, viability Abstract: Poor and irregular germiniition of large cardamom seed become a problem of
nursery growers and recalcitrant nature and dormancy are believed to be the
factors responsible with it. An experiment was carried for three consecutive years
to identify the seed viability period stored in different conditions with different seed
moisture level using tetrazolium test. The ·results showed that the seed remain
viable (>80%) even dried to less than 11% moisture level in electric oven. There
was non-significant difference in seed viability stored under refrigerator and
ambient condition till 150 days after seed drying by both methods. However, effect
of storage duration was prominent (p>.001) where viability was not statistically
different between 15 and 60 days after drying. The result has clearly proved that
large cardamom seed has orthodox behavior of storage despite farmers' notion of
recalcitrant nature of the seed. In addition to th(s finding, light has been shed to the
other basic information of large cardamom seed.Link for e-copy: http://elibrary.narc.gov.np/?r=252 E-copies
http://nkcs.org.np/narc/dl/?r=252URL