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Optimization of PCR Conditions for DNA Amplification of Common Buckwheat Using EST Primers / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 8 (2007)
[article]
Title : Optimization of PCR Conditions for DNA Amplification of Common Buckwheat Using EST Primers Material Type: printed text Authors: Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; T. Hara, Author Publication Date: 2007 Article on page: 1-6 p. Languages : English (eng) Keywords: Annealing temperature, buckwheat, EST markers, optimization of PCRconditions Abstract: Under optimal conditions the PCR reaction is very efficient; microgram quantities may be
synthesized from a single molecule of substrate DNA. DNA of four lines of common
buckwheat (Kyusu, Canada, Miyazaki and Botansoba) was used to optimize PCR reaction
and cycling program of 26 primers for DNA amplification of common buckwheat.
Annealing temperature (Ta), PCR cycle number and MgCl2 concentration were considered
optimum if the single clear band was observed. Of the 26 primers Ta of only 10 primers
could be optimized. Three primer pairs performed best at Ta of 54oC. The optimum
concentration of MgCl2 was found to be 1.5mM for all primer pairs. Similarly the number of
PCR cycles was found to be 40 for all 10 primer pairs except for primer pair 57. Optimized
PCR conditions were used for subsequent studies such as transferability of EST primers to
other Fagopyrum species and construction of linkage map.Link for e-copy: http://elibrary.narc.gov.np/?r=17
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 1-6 p.[article] Optimization of PCR Conditions for DNA Amplification of Common Buckwheat Using EST Primers [printed text] / Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; T. Hara, Author . - 2007 . - 1-6 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 1-6 p.
Keywords: Annealing temperature, buckwheat, EST markers, optimization of PCRconditions Abstract: Under optimal conditions the PCR reaction is very efficient; microgram quantities may be
synthesized from a single molecule of substrate DNA. DNA of four lines of common
buckwheat (Kyusu, Canada, Miyazaki and Botansoba) was used to optimize PCR reaction
and cycling program of 26 primers for DNA amplification of common buckwheat.
Annealing temperature (Ta), PCR cycle number and MgCl2 concentration were considered
optimum if the single clear band was observed. Of the 26 primers Ta of only 10 primers
could be optimized. Three primer pairs performed best at Ta of 54oC. The optimum
concentration of MgCl2 was found to be 1.5mM for all primer pairs. Similarly the number of
PCR cycles was found to be 40 for all 10 primer pairs except for primer pair 57. Optimized
PCR conditions were used for subsequent studies such as transferability of EST primers to
other Fagopyrum species and construction of linkage map.Link for e-copy: http://elibrary.narc.gov.np/?r=17
in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.
Title : Testing and evaluation of Mini SRR Dryer in Nepal for rice crop Material Type: printed text Authors: G.B Manandhar, Author ; Rabi Rasaily, Author ; Shrestha, G.L., Author ; R.R Bakker, Author Pagination: 19-27 p. Languages : English (eng) Keywords: Drying air temperature, drying cost, rate, moisture content, payback period Abstract: Mini SRR rice seed dryer received from IRRI consisted of a cylindrical bin, an electric blower (2.5”, 320 watt) with a cylindrical support, a rice husk stove and a metallic pipe. Eight tests were conducted during rainy weeks of Ju ly ,2002 three at Kavre Palanchowk, four at Dhanusha and one at Parsa district Grain loaded varied from 145 to 175 kg with moisture content (MC) from 17.8 to 23.5%. Overall drying rate with rice husk as source of heat was found to be 0.45% MC/hour, which is much higher, compared to results of Vietnamese SRR-1 dryer with electricity as heat source. Extrapolation to 13% final MC by simple linear regression showed an association with one another in a linear way (r = 0.83). With 250 kg loading, drying cost for one ton rice was found to be Rs476 for IRRI dryer but in replicated dryer without heat insulation on metal pipe, it was found to be Rs874 due to lower blower RPM. However, computations show that even cost of replicated dryer would be paid back within 10 years with 35 days of operation per year. It showed the possibility of commercializing t he dryer for the small farmers either for seed or for food grain purpose. Maximum thermal efficiency of 22.4% was obtained at 0.6 kg rice husk/hour and 44.7°C drying air temperature (DAT). With DAT from 44.7°C to 49.0°C at Kavre germination rate of paddy varied from 77-88%. The study, also aimed a t enhancing the coverage of early rice in the country, has shown encouraging result.
Link for e-copy: http://elibrary.narc.gov.np/?r=863 Testing and evaluation of Mini SRR Dryer in Nepal for rice crop [printed text] / G.B Manandhar, Author ; Rabi Rasaily, Author ; Shrestha, G.L., Author ; R.R Bakker, Author . - [s.d.] . - 19-27 p.
in Agricultural research for enhancing livelihood of Nepalese people: Proceedings of 2nd SAS-N Convention, 30 July-1 Aug 2003, Kathmandu / Joshi, Bal K.
Languages : English (eng)
Keywords: Drying air temperature, drying cost, rate, moisture content, payback period Abstract: Mini SRR rice seed dryer received from IRRI consisted of a cylindrical bin, an electric blower (2.5”, 320 watt) with a cylindrical support, a rice husk stove and a metallic pipe. Eight tests were conducted during rainy weeks of Ju ly ,2002 three at Kavre Palanchowk, four at Dhanusha and one at Parsa district Grain loaded varied from 145 to 175 kg with moisture content (MC) from 17.8 to 23.5%. Overall drying rate with rice husk as source of heat was found to be 0.45% MC/hour, which is much higher, compared to results of Vietnamese SRR-1 dryer with electricity as heat source. Extrapolation to 13% final MC by simple linear regression showed an association with one another in a linear way (r = 0.83). With 250 kg loading, drying cost for one ton rice was found to be Rs476 for IRRI dryer but in replicated dryer without heat insulation on metal pipe, it was found to be Rs874 due to lower blower RPM. However, computations show that even cost of replicated dryer would be paid back within 10 years with 35 days of operation per year. It showed the possibility of commercializing t he dryer for the small farmers either for seed or for food grain purpose. Maximum thermal efficiency of 22.4% was obtained at 0.6 kg rice husk/hour and 44.7°C drying air temperature (DAT). With DAT from 44.7°C to 49.0°C at Kavre germination rate of paddy varied from 77-88%. The study, also aimed a t enhancing the coverage of early rice in the country, has shown encouraging result.
Link for e-copy: http://elibrary.narc.gov.np/?r=863 E-copies
http://nkcs.org.np/narc/dl/?r=863URL Variability in Phase Transition and Contribution of Genetic Variance to Yield in Wheat at High Altitude / Barid B Bandyopadhyay in Nepal Agriculture Research Journal, Vol.11 (2011)
[article]
Title : Variability in Phase Transition and Contribution of Genetic Variance to Yield in Wheat at High Altitude Material Type: printed text Authors: Barid B Bandyopadhyay, Author Publication Date: 2011 Article on page: 21-27p. Languages : English (eng) Keywords: High altitude, Low temperature, Water deficit stress, Wheat cultivars Link for e-copy: http://elibrary.narc.gov.np/?r=1190
in Nepal Agriculture Research Journal > Vol.11 (2011) . - 21-27p.[article] Variability in Phase Transition and Contribution of Genetic Variance to Yield in Wheat at High Altitude [printed text] / Barid B Bandyopadhyay, Author . - 2011 . - 21-27p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol.11 (2011) . - 21-27p.
Keywords: High altitude, Low temperature, Water deficit stress, Wheat cultivars Link for e-copy: http://elibrary.narc.gov.np/?r=1190