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Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 7 (2006)
[article]
Title : Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum Material Type: printed text Authors: Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; Ryo Ohsawa, Author Publication Date: 2006 Article on page: 27-36 p Languages : English (eng) Keywords: Common buckwheat, EST markers, Fagopyrum species, transferability Abstract: If the EST primers designed for one species can be used in related species, then the cost involved
in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for
other species is significantly reduced. We tested the applicability of 17 EST primers developed
from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18
accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones
were used to design primers using Primer3. Amplification products were different in band
intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All
primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced
very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight
primers amplified the DNA of all accessions. Results indicated that the transferability of EST
markers developed for common buckwheat decreased with an increase in genetic distance
between them.Link for e-copy: http://elibrary.narc.gov.np/?r=43
in Nepal Agriculture Research Journal > Vol. 7 (2006) . - 27-36 p[article] Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum [printed text] / Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; Ryo Ohsawa, Author . - 2006 . - 27-36 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 7 (2006) . - 27-36 p
Keywords: Common buckwheat, EST markers, Fagopyrum species, transferability Abstract: If the EST primers designed for one species can be used in related species, then the cost involved
in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for
other species is significantly reduced. We tested the applicability of 17 EST primers developed
from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18
accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones
were used to design primers using Primer3. Amplification products were different in band
intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All
primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced
very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight
primers amplified the DNA of all accessions. Results indicated that the transferability of EST
markers developed for common buckwheat decreased with an increase in genetic distance
between them.Link for e-copy: http://elibrary.narc.gov.np/?r=43 Utilizing invasive plant species,eupatorium for increasing land productivity through making Biochar in Nepal / N.R Pandit
in Proceedings of the Second National Soil Fertility Research Workshop, 24-25 March, 2015. Soil Science Division, NARC, Khumaltar, Lalitpur, Nepal / Krishna B. Karki
Title : Utilizing invasive plant species,eupatorium for increasing land productivity through making Biochar in Nepal Material Type: printed text Authors: N.R Pandit, Author ; B.H Pandit, Author ; H.P Schmidt, Author Pagination: 101-110 p. Languages : English (eng) Keywords: Biochar application, cost benefit analysis, invasive plant species, pyrolysis, sustainable rural ecology for green growth. Abstract: The majorities of poor people in Nepal rely mostly on agriculture for employment and spend a high proportion of their income on food. Population densities continue to increase and resource available for maintaining people’s livelihood is becoming increasingly scarce. The available lands have decreased their productivity, which has not been able to address livelihoods and food security issues. Thus sustainable increase in land productivity in agriculture, through effective use of underutilized resources such as crop residues, rice husk, animal left over and biomass of invasive plant species (such as Eupatorium) continue to be crucial means through which both poverty reduction and economic growth are sought. In order to address this issue, ADB funded project, Sustainable Rural Ecology for Green Growth has tested the feasibility of using biomass of Eupatorium species for making biochar that refers to materials produced through Pyrolysis…………………
Link for e-copy: http://elibrary.narc.gov.np/?r=1789 Utilizing invasive plant species,eupatorium for increasing land productivity through making Biochar in Nepal [printed text] / N.R Pandit, Author ; B.H Pandit, Author ; H.P Schmidt, Author . - [s.d.] . - 101-110 p.
in Proceedings of the Second National Soil Fertility Research Workshop, 24-25 March, 2015. Soil Science Division, NARC, Khumaltar, Lalitpur, Nepal / Krishna B. Karki
Languages : English (eng)
Keywords: Biochar application, cost benefit analysis, invasive plant species, pyrolysis, sustainable rural ecology for green growth. Abstract: The majorities of poor people in Nepal rely mostly on agriculture for employment and spend a high proportion of their income on food. Population densities continue to increase and resource available for maintaining people’s livelihood is becoming increasingly scarce. The available lands have decreased their productivity, which has not been able to address livelihoods and food security issues. Thus sustainable increase in land productivity in agriculture, through effective use of underutilized resources such as crop residues, rice husk, animal left over and biomass of invasive plant species (such as Eupatorium) continue to be crucial means through which both poverty reduction and economic growth are sought. In order to address this issue, ADB funded project, Sustainable Rural Ecology for Green Growth has tested the feasibility of using biomass of Eupatorium species for making biochar that refers to materials produced through Pyrolysis…………………
Link for e-copy: http://elibrary.narc.gov.np/?r=1789 E-copies
http://nkcs.org.np/narc/dl/?r=1789URL