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3 result(s) search for keyword(s) 'pre-basic'
Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal / Sakha, B.M. in Nepal Agriculture Research Journal, Vol. 8 (2007)
[article]
Title : Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal Material Type: printed text Authors: Sakha, B.M., Author ; GP Rai, Author ; Dhital, S.P., Author Publication Date: 2007 Article on page: 7-13 p Languages : English (eng) Keywords: DAS-ELISA, meristem excision, potato, pre-basic seed, thermotherapy Abstract: Pre-basic seed potatoes are disease free potato minitubers produced by transplanting
pathogen free in vitro potato plantlets under protected condition in aphid-proof glasshouse
and/or screen house. Double antibody sand witched - enzyme linked immuno-sorbant assay
is used to test six major potato viruses, namely PLRV, PVS, PVX, PVY, PVA and PVM.
Thermotherapy cum meristem tip excision techniques are used to eliminate these viruses.
Virus free in vitro potato plantlets are rapidly propagated by single nodal cuttings on
modified MS media. For pre-basic seed production disease free in vitro potato plantlets are
transplanted in the sterile sand soil substrate under glasshouse and screen house, once in
autumn season and next in spring season. Since 1990, National Potato Research Program has
been producing about 200,000 pre-basic seeds annually. So far, PBS of 19 different
recommended and released potato cultivars has been produced. Till date 3,465,799 PBS had
been produced and 3,217,666 pre-basic seeds distributed to the different seed potato growers
groups, District Agriculture Development Offices, government farms/research stations, and
NGOs/INGOs for subsequent basic seed potato production. After establishment of tissue
culture facilities in National Potato Research Program, the productivity of potato has been
increased by 71% due to utilization of pre-basic seed potatoes.Link for e-copy: http://elibrary.narc.gov.np/?r=20
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 7-13 p[article] Disease-free Pre-Basic Seed Potato Production through Tissue Culture in Nepal [printed text] / Sakha, B.M., Author ; GP Rai, Author ; Dhital, S.P., Author . - 2007 . - 7-13 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 8 (2007) . - 7-13 p
Keywords: DAS-ELISA, meristem excision, potato, pre-basic seed, thermotherapy Abstract: Pre-basic seed potatoes are disease free potato minitubers produced by transplanting
pathogen free in vitro potato plantlets under protected condition in aphid-proof glasshouse
and/or screen house. Double antibody sand witched - enzyme linked immuno-sorbant assay
is used to test six major potato viruses, namely PLRV, PVS, PVX, PVY, PVA and PVM.
Thermotherapy cum meristem tip excision techniques are used to eliminate these viruses.
Virus free in vitro potato plantlets are rapidly propagated by single nodal cuttings on
modified MS media. For pre-basic seed production disease free in vitro potato plantlets are
transplanted in the sterile sand soil substrate under glasshouse and screen house, once in
autumn season and next in spring season. Since 1990, National Potato Research Program has
been producing about 200,000 pre-basic seeds annually. So far, PBS of 19 different
recommended and released potato cultivars has been produced. Till date 3,465,799 PBS had
been produced and 3,217,666 pre-basic seeds distributed to the different seed potato growers
groups, District Agriculture Development Offices, government farms/research stations, and
NGOs/INGOs for subsequent basic seed potato production. After establishment of tissue
culture facilities in National Potato Research Program, the productivity of potato has been
increased by 71% due to utilization of pre-basic seed potatoes.Link for e-copy: http://elibrary.narc.gov.np/?r=20 Effects of Season on Rapid Multiplication of Virus-free Plant-lets under in Vitro and on Production of Pre-basic Seeds of Potato(Solanum tuberosum L.) under In Vivo Conditions / Shambhu P. Dhital in Nepal Agriculture Research Journal, Vol.10 (2010)
[article]
Title : Effects of Season on Rapid Multiplication of Virus-free Plant-lets under in Vitro and on Production of Pre-basic Seeds of Potato(Solanum tuberosum L.) under In Vivo Conditions Material Type: printed text Authors: Shambhu P. Dhital, Author ; Binesh M. Sakha, Author ; Ram B. Nepal, Author Publication Date: 2010 Article on page: 75-82 p. Languages : English (eng) Keywords: Autumn season, Glasshouse, Screenhouse, Size of pre-basic seed,Spring season Link for e-copy: http://elibrary.narc.gov.np/?r=1206
in Nepal Agriculture Research Journal > Vol.10 (2010) . - 75-82 p.[article] Effects of Season on Rapid Multiplication of Virus-free Plant-lets under in Vitro and on Production of Pre-basic Seeds of Potato(Solanum tuberosum L.) under In Vivo Conditions [printed text] / Shambhu P. Dhital, Author ; Binesh M. Sakha, Author ; Ram B. Nepal, Author . - 2010 . - 75-82 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol.10 (2010) . - 75-82 p.
Keywords: Autumn season, Glasshouse, Screenhouse, Size of pre-basic seed,Spring season Link for e-copy: http://elibrary.narc.gov.np/?r=1206 Lowcost Production of In Vitro Plantlets for Sustainable Pre-basic Seed Potato Production In Nepal / Binesh M. Sakha ; Gyan P. Rai ; Shambhu P. Dhital
in Proceedings of the fifth National Seminar on Horticulture June 9-10, 2008 / Nepal Agricultural Research Council (NARC) (Khumaltar, Lalitpur, Nepal)
Title : Lowcost Production of In Vitro Plantlets for Sustainable Pre-basic Seed Potato Production In Nepal Material Type: printed text Authors: Binesh M. Sakha, Author ; Gyan P. Rai, Author ; Shambhu P. Dhital, Author Pagination: 162-167 p. Languages : English (eng) Keywords: intensive culture, in vitro, mini tubers, pre-basic seed, sustainable Abstract: In vitro and in vivo experiments were conducted in the tissue culture laboratory and
glasshouse of National Potato Research Programme, Khumaltar during 1997/98 to
minimize the cost of in vitro potato plantlets production. Use of sugar as substitute for
sucrose in culture showed no difference in the growth performance of in vitro potato
plantlets, and even in their yield performance under glasshouse condition. Culture of in
vitro potato plantlets on test tubes at the rate of 40 culture tubes per tray of 50 tubes
capacity was efficient than raising usual 30 culture tubes per tray, since both produced
more vigorous plantlets with higher nodal explants harvest as compared to culturing 50
tubes per tray. Culturing of 10 plantlets per bottle produced higher proportion of usable
nodal explants as compared to plantlets cultured at the rate of 12 and 15 plantlets per
bottle. Therefore, for the sustainable PBS production, sucrose could be substituted by
sugar in the cultures medium, in vitro potato plantlets subculture on trays at the rate of
40 tubes per tray and final multiplication of cultures on jam bottles at the rate .of 10
plantlets per bottle. A 37% cost reduction in the in vitro culture of potato was achieved
by intensive culture of plantlets on jam bottles.Link for e-copy: http://elibrary.narc.gov.np/?r=240 Lowcost Production of In Vitro Plantlets for Sustainable Pre-basic Seed Potato Production In Nepal [printed text] / Binesh M. Sakha, Author ; Gyan P. Rai, Author ; Shambhu P. Dhital, Author . - [s.d.] . - 162-167 p.
in Proceedings of the fifth National Seminar on Horticulture June 9-10, 2008 / Nepal Agricultural Research Council (NARC) (Khumaltar, Lalitpur, Nepal)
Languages : English (eng)
Keywords: intensive culture, in vitro, mini tubers, pre-basic seed, sustainable Abstract: In vitro and in vivo experiments were conducted in the tissue culture laboratory and
glasshouse of National Potato Research Programme, Khumaltar during 1997/98 to
minimize the cost of in vitro potato plantlets production. Use of sugar as substitute for
sucrose in culture showed no difference in the growth performance of in vitro potato
plantlets, and even in their yield performance under glasshouse condition. Culture of in
vitro potato plantlets on test tubes at the rate of 40 culture tubes per tray of 50 tubes
capacity was efficient than raising usual 30 culture tubes per tray, since both produced
more vigorous plantlets with higher nodal explants harvest as compared to culturing 50
tubes per tray. Culturing of 10 plantlets per bottle produced higher proportion of usable
nodal explants as compared to plantlets cultured at the rate of 12 and 15 plantlets per
bottle. Therefore, for the sustainable PBS production, sucrose could be substituted by
sugar in the cultures medium, in vitro potato plantlets subculture on trays at the rate of
40 tubes per tray and final multiplication of cultures on jam bottles at the rate .of 10
plantlets per bottle. A 37% cost reduction in the in vitro culture of potato was achieved
by intensive culture of plantlets on jam bottles.Link for e-copy: http://elibrary.narc.gov.np/?r=240 E-copies
http://nkcs.org.np/narc/dl/?r=240URL