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Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum / Joshi, Bal K. in Nepal Agriculture Research Journal, Vol. 7 (2006)
[article]
Title : Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum Material Type: printed text Authors: Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; Ryo Ohsawa, Author Publication Date: 2006 Article on page: 27-36 p Languages : English (eng) Keywords: Common buckwheat, EST markers, Fagopyrum species, transferability Abstract: If the EST primers designed for one species can be used in related species, then the cost involved
in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for
other species is significantly reduced. We tested the applicability of 17 EST primers developed
from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18
accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones
were used to design primers using Primer3. Amplification products were different in band
intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All
primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced
very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight
primers amplified the DNA of all accessions. Results indicated that the transferability of EST
markers developed for common buckwheat decreased with an increase in genetic distance
between them.Link for e-copy: http://elibrary.narc.gov.np/?r=43
in Nepal Agriculture Research Journal > Vol. 7 (2006) . - 27-36 p[article] Common Buckwheat-based EST Primers in the Genome of other Species of Fagopyrum [printed text] / Joshi, Bal K., Author ; Kazutoshi, Okuno, Author ; Ryo Ohsawa, Author . - 2006 . - 27-36 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 7 (2006) . - 27-36 p
Keywords: Common buckwheat, EST markers, Fagopyrum species, transferability Abstract: If the EST primers designed for one species can be used in related species, then the cost involved
in developing markers for DNA fingerprinting, genetic relationship studies, mapping, etc. for
other species is significantly reduced. We tested the applicability of 17 EST primers developed
from common buckwheat in other wild and cultivated Fagopyrum species. A total of 18
accessions consisting of 4 subspecies and 2 species were used. Sequences of 93 cDNA clones
were used to design primers using Primer3. Amplification products were different in band
intensity. In most of the cases, the bands of F. homotropicum were with high intensity. All
primers showed single band except in Accession C9022. Three primers 23, 31 and 69 produced
very clear singe band. All primers amplified the genomic DNA of F. homotropicum (2x). Eight
primers amplified the DNA of all accessions. Results indicated that the transferability of EST
markers developed for common buckwheat decreased with an increase in genetic distance
between them.Link for e-copy: http://elibrary.narc.gov.np/?r=43 Intensity of Common Root Rot on Wheat Genotypes / Deepak Bhandari in Nepal Agriculture Research Journal, Vol. 5 (2004)
[article]
Title : Intensity of Common Root Rot on Wheat Genotypes Material Type: printed text Authors: Deepak Bhandari, Author ; Sundar M. Shrestha, Author Publication Date: 2004 Article on page: 46-48 p Languages : English (eng) Keywords: Bipolaris sorokiniana, common root rot, dry land wheat Abstract: Common root rot of wheat caused by Bipolaris sorokiniana (Sacc. in Sorok.) has been an
emerging problem in Nepal. This study was conducted to determine the level of resistance in
promising wheat genotypes. Thirty wheat genotypes were tested under natural epiphytotic
condition for the intensity of infection on subcrown internode and crown root. None of the wheat
varieties were found immune common root rot. However, genotype BL 2246, BL 2203, CHAM-4
and BL 2262 had low level of infection denoting resistant character. Most of the genotypes had
moderate level of root rot index that ranged from 20-35 percent. Genotypes BL 2302, BL 1724
had more than 50 percent root rot index. The resistant genotypes have to be further tested under
artificial and sick plot condition for confirmation of the above result.Link for e-copy: http://elibrary.narc.gov.np/?r=78
in Nepal Agriculture Research Journal > Vol. 5 (2004) . - 46-48 p[article] Intensity of Common Root Rot on Wheat Genotypes [printed text] / Deepak Bhandari, Author ; Sundar M. Shrestha, Author . - 2004 . - 46-48 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 5 (2004) . - 46-48 p
Keywords: Bipolaris sorokiniana, common root rot, dry land wheat Abstract: Common root rot of wheat caused by Bipolaris sorokiniana (Sacc. in Sorok.) has been an
emerging problem in Nepal. This study was conducted to determine the level of resistance in
promising wheat genotypes. Thirty wheat genotypes were tested under natural epiphytotic
condition for the intensity of infection on subcrown internode and crown root. None of the wheat
varieties were found immune common root rot. However, genotype BL 2246, BL 2203, CHAM-4
and BL 2262 had low level of infection denoting resistant character. Most of the genotypes had
moderate level of root rot index that ranged from 20-35 percent. Genotypes BL 2302, BL 1724
had more than 50 percent root rot index. The resistant genotypes have to be further tested under
artificial and sick plot condition for confirmation of the above result.Link for e-copy: http://elibrary.narc.gov.np/?r=78 Effect of Different Feed Ingredients on the Growth of Caged Common Carp / Rai, Ash Kumar in Nepal Agriculture Research Journal, Vol. 4 (2000/2001)
[article]
Title : Effect of Different Feed Ingredients on the Growth of Caged Common Carp Material Type: printed text Authors: Rai, Ash Kumar, Author ; Jaya D. Bista, Author Publication Date: 2000 Article on page: 60-63 p Languages : English (eng) Keywords: Cage fish culture, common carp, feed ingredients Abstract: To know the effect of four different fish feed, nine months old common carp about 47 g size were
stocked at the stocking density of 12 fish m-3 in the cages and cultured in Lake Phewa for 175
days. Four different feed ingredients used were fish meal + soybean + oil cake + rice bran + wheat
flour, fish meal + oil cake + rice bran + wheat flour, soybean + oil cake + rice bran + wheat flour
and commercial cattle feed. The crude protein ranges from 23 to 32.2%. 32.2% protein content
feed containing ingredients of fish meal + soybean + oil cake + rice bran + wheat flour was better
for the fish growth (0.30 g day-1) but was the lowest survival rate (65.4%) followed by 27.1%
protein content feed containing fish meal + oil cake + rice bran + wheat flour (0.23 g day-1). 26.2%
protein content feed containing soybean + oil cake + rice bran + wheat flour without animal
protein was the poorest growth rate (0.17 g day-1) with the highest survival rate (95.7%) among the
tested feed. It was observed that feed with higher protein level was better for the fish growth and
the growth of the fish was different significantly among the treatments except the treatment fish
meal + oil cake + rice bran + wheat flour and commercial cattle feed. The common carp fish
cultured in cages through artificial feed was not satisfactory. It digs and burrows the pond
embankments and sides in search of organic matter that makes pond turbid.Link for e-copy: http://elibrary.narc.gov.np/?r=95
in Nepal Agriculture Research Journal > Vol. 4 (2000/2001) . - 60-63 p[article] Effect of Different Feed Ingredients on the Growth of Caged Common Carp [printed text] / Rai, Ash Kumar, Author ; Jaya D. Bista, Author . - 2000 . - 60-63 p.
Languages : English (eng)
in Nepal Agriculture Research Journal > Vol. 4 (2000/2001) . - 60-63 p
Keywords: Cage fish culture, common carp, feed ingredients Abstract: To know the effect of four different fish feed, nine months old common carp about 47 g size were
stocked at the stocking density of 12 fish m-3 in the cages and cultured in Lake Phewa for 175
days. Four different feed ingredients used were fish meal + soybean + oil cake + rice bran + wheat
flour, fish meal + oil cake + rice bran + wheat flour, soybean + oil cake + rice bran + wheat flour
and commercial cattle feed. The crude protein ranges from 23 to 32.2%. 32.2% protein content
feed containing ingredients of fish meal + soybean + oil cake + rice bran + wheat flour was better
for the fish growth (0.30 g day-1) but was the lowest survival rate (65.4%) followed by 27.1%
protein content feed containing fish meal + oil cake + rice bran + wheat flour (0.23 g day-1). 26.2%
protein content feed containing soybean + oil cake + rice bran + wheat flour without animal
protein was the poorest growth rate (0.17 g day-1) with the highest survival rate (95.7%) among the
tested feed. It was observed that feed with higher protein level was better for the fish growth and
the growth of the fish was different significantly among the treatments except the treatment fish
meal + oil cake + rice bran + wheat flour and commercial cattle feed. The common carp fish
cultured in cages through artificial feed was not satisfactory. It digs and burrows the pond
embankments and sides in search of organic matter that makes pond turbid.Link for e-copy: http://elibrary.narc.gov.np/?r=95 Muluki ain :Yek tippani / Shrestha, Gyaindrabahadur
Title : Muluki ain :Yek tippani Material Type: printed text Authors: Shrestha, Gyaindrabahadur ; , Edition statement: 2nd Publisher: Kathmandu : Pairabi Prakashan Publication Date: 2052 Pagination: 552 General note: AN: 4284 Keywords: Common law; Law Class number: N.340.549657 Muluki ain :Yek tippani [printed text] / Shrestha, Gyaindrabahadur ; , . - 2nd . - Kathmandu : Pairabi Prakashan, 2052 . - 552.
AN: 4284
Keywords: Common law; Law Class number: N.340.549657 Copies (1)
Barcode Call number Media type Location Section Status NARC1004284 N.340.549657, SHR Document NARC Library Documents Available