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Meristem Culture of Mandarin Orange (Citrus reticulata) for Elimination of Citrus Tristeza Virus / Radha Panthi in Nepalese Horticulture, v. 8/9 ([07/25/2012])
[article]
Title : Meristem Culture of Mandarin Orange (Citrus reticulata) for Elimination of Citrus Tristeza Virus Material Type: printed text Authors: Radha Panthi, Author ; Ranjit, Mukunda, Author Publication Date: 2012 Article on page: 63-68 p. Languages : English (eng) Keywords: CTV, DAS-ELISA, Meristem Tissue Culture, In vitro Abstract: Mandarin trees grafted on trifoliate orange showing symptoms of citrus tristeza
virus (CTV) such as yellowing of leaves, interveinal chlorosis and stem pitting
were subjected to virus testing by double antibody sandwich enzyme-linked
immuno sorbent assay (DAS-ELISA). Results with optical density (OD405) of
more than 0.5 have confirmed the presence of CTV. Culture of apical meristems
with few leaf primordia ranging from 0.2 to 0.5 mm in diameter has given rise to
virus-free plantlets (OD405 of 0.16) after eight weeks. This technique of
meristem culture may be useful to eliminate viruses from elite mandarin plants.
in Nepalese Horticulture > v. 8/9 [07/25/2012] . - 63-68 p.[article] Meristem Culture of Mandarin Orange (Citrus reticulata) for Elimination of Citrus Tristeza Virus [printed text] / Radha Panthi, Author ; Ranjit, Mukunda, Author . - 2012 . - 63-68 p.
Languages : English (eng)
in Nepalese Horticulture > v. 8/9 [07/25/2012] . - 63-68 p.
Keywords: CTV, DAS-ELISA, Meristem Tissue Culture, In vitro Abstract: Mandarin trees grafted on trifoliate orange showing symptoms of citrus tristeza
virus (CTV) such as yellowing of leaves, interveinal chlorosis and stem pitting
were subjected to virus testing by double antibody sandwich enzyme-linked
immuno sorbent assay (DAS-ELISA). Results with optical density (OD405) of
more than 0.5 have confirmed the presence of CTV. Culture of apical meristems
with few leaf primordia ranging from 0.2 to 0.5 mm in diameter has given rise to
virus-free plantlets (OD405 of 0.16) after eight weeks. This technique of
meristem culture may be useful to eliminate viruses from elite mandarin plants.Management of citrus decline and rejuvenation strategies for declining orchards of Dailekh / Subedi, G.D.
in Agricultural research for poverty alleviation and livelihood enhancement. Proceedings of the 3rd SAS-N Convention 27-29 August 2008, Lalitpur / Paudyal, Krishna Prasad
Title : Management of citrus decline and rejuvenation strategies for declining orchards of Dailekh Material Type: printed text Authors: Subedi, G.D., Author ; U. K. Acharya, Author Pagination: 141-150 p. Languages : English (eng) Keywords: Citrus decline, CTV, greening disease, Diaphorina citri, PCR analysi Abstract: A participatory rural appraisal (PRA) was conducted by Agricultural Research Station (Horticulture), Dailekh during 2006/2007 at major citrus pockets viz Dullu, Mallika, Gamaudi, Kal Bhairav, Lakuri, Goganpani and Kandechaur to collect information regarding the incidence of citrus decline along with general cultivation practices followed by the citrus growers……………….
Link for e-copy: http://elibrary.narc.gov.np/?r=1516 Management of citrus decline and rejuvenation strategies for declining orchards of Dailekh [printed text] / Subedi, G.D., Author ; U. K. Acharya, Author . - [s.d.] . - 141-150 p.
in Agricultural research for poverty alleviation and livelihood enhancement. Proceedings of the 3rd SAS-N Convention 27-29 August 2008, Lalitpur / Paudyal, Krishna Prasad
Languages : English (eng)
Keywords: Citrus decline, CTV, greening disease, Diaphorina citri, PCR analysi Abstract: A participatory rural appraisal (PRA) was conducted by Agricultural Research Station (Horticulture), Dailekh during 2006/2007 at major citrus pockets viz Dullu, Mallika, Gamaudi, Kal Bhairav, Lakuri, Goganpani and Kandechaur to collect information regarding the incidence of citrus decline along with general cultivation practices followed by the citrus growers……………….
Link for e-copy: http://elibrary.narc.gov.np/?r=1516 E-copies
http://nkcs.org.np/narc/dl/?r=1516URL