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Molecular screening for rust resistance characteristics in Nepalese barley germplasm. / Madan Raj Bhatta
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Molecular screening for rust resistance characteristics in Nepalese barley germplasm. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Sah, Bindeshwar P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 35-43 p. Languages : English (eng) Keywords: 'stripe rust', 'Puccinia', 'resistant', 'durable', 'gene' Abstract: Leaf rust ( caused by Puccinia hordei) and stripe/yellow rust ( caused by P. striformis fsp. hordei and P. striformis fsp. tritici) are the major problem for barley cultivation in Nepal. Durable resistant variety of the barley is highly important for this country. Barley rust resistance is controlled by many major genes and quantitative trait loci (QTLs) and pyramiding these genes into varieties is the most important barley breeding strategies in Nepal. Molecular marker assisted breeding (MAB) is the only option for this. Marker assisted screening is the primary stage to initiate the MAB. One hundred and nine Nepalese barley accessions were screened using six and eleven SSR markers linked to leaf rust and stripe rust resistance genes respectively. Bonus and Local Jau was used as the resistant and susceptible check respectively. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR-P and QTL on 5HS was detected on Nepalese barley accessions using respective SSR markers. Similarly, stripe rust resistance genes QTL on lH, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were detected. Eight Nepalese barley accessions showed presence of three and more leaf rust resistant genes and twenty-five Nepalese barley accessions have three and more stripe rust resistance genes. Poor relationship between rust resistance gene and field disease may be due to the less relationship between the pathogen type on field and rust resistance genes detected using SSR markers on Nepalese barley germplasm.
Link for e-copy: http://elibrary.narc.gov.np/?r=1491 Molecular screening for rust resistance characteristics in Nepalese barley germplasm. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Sah, Bindeshwar P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 35-43 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'stripe rust', 'Puccinia', 'resistant', 'durable', 'gene' Abstract: Leaf rust ( caused by Puccinia hordei) and stripe/yellow rust ( caused by P. striformis fsp. hordei and P. striformis fsp. tritici) are the major problem for barley cultivation in Nepal. Durable resistant variety of the barley is highly important for this country. Barley rust resistance is controlled by many major genes and quantitative trait loci (QTLs) and pyramiding these genes into varieties is the most important barley breeding strategies in Nepal. Molecular marker assisted breeding (MAB) is the only option for this. Marker assisted screening is the primary stage to initiate the MAB. One hundred and nine Nepalese barley accessions were screened using six and eleven SSR markers linked to leaf rust and stripe rust resistance genes respectively. Bonus and Local Jau was used as the resistant and susceptible check respectively. Leaf rust resistance gene Rphl, Rph2, Rph3, Rph7, QBLR-P and QTL on 5HS was detected on Nepalese barley accessions using respective SSR markers. Similarly, stripe rust resistance genes QTL on lH, QTL5 on lH, QTL on 4H, QTL on SH, QTL on 6H, QTL on 7H, YrpstYl and Rpsx were detected. Eight Nepalese barley accessions showed presence of three and more leaf rust resistant genes and twenty-five Nepalese barley accessions have three and more stripe rust resistance genes. Poor relationship between rust resistance gene and field disease may be due to the less relationship between the pathogen type on field and rust resistance genes detected using SSR markers on Nepalese barley germplasm.
Link for e-copy: http://elibrary.narc.gov.np/?r=1491 E-copies
http://nkcs.org.np/narc/dl/?r=1491URL Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. / Madan Raj Bhatta
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 18-22 p. Languages : English (eng) Keywords: 'durable', 'resistant', 'susceptible', 'pathotype', 'pyramiding' Abstract: Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on the availability and use of resistant gene donors. Ninety six Nepalese rice accessions were screened using eight SSR markers and one STS marker for presence and absence of BLB resistance gene. We detected BLB resistance gene Xa-10 on two accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-10 on five accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that different BLB pathotypes are active in Nepalese rice field and need of gene pyramiding for durable resistance.
Link for e-copy: http://elibrary.narc.gov.np/?r=1487 Screening bacterial leaf blight (BLB) resistance gene in Nepalese rice gene pool using molecular markers. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Niroula, Raj.K, Author ; Sah, Bindeshwar P., Author ; Bishal G. Tarnang, Author ; Hamal, Shreya S., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 18-22 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'durable', 'resistant', 'susceptible', 'pathotype', 'pyramiding' Abstract: Bacterial Leaf Blight (BLB) is the most important yield limiting factor in Nepalese rice. BLB resistance rice varieties are highly demanding in the country. Breeding efforts for developing disease resistant depends on the availability and use of resistant gene donors. Ninety six Nepalese rice accessions were screened using eight SSR markers and one STS marker for presence and absence of BLB resistance gene. We detected BLB resistance gene Xa-10 on two accessions, Xa-13 on six accessions, Xa-7 on 23 accessions, Xa-10 on five accessions, Xa-3 and Xa-4 on 52 accessions, Xa-5 on 25 accessions, Xa-8 on 30 rice accessions. No any rice accessions have Xa-21. Similarly, 17 rice accessions showed three and more than three BLB resistance genes. Presence of Xa-13 on susceptible check variety CNTRL-85033 confirmed that different BLB pathotypes are active in Nepalese rice field and need of gene pyramiding for durable resistance.
Link for e-copy: http://elibrary.narc.gov.np/?r=1487 E-copies
http://nkcs.org.np/narc/dl/?r=1487URL
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Title : Variation on rust resistance characteristics on Nepalese barley germplasm. Material Type: printed text Authors: Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author Publisher: Lalitpur : Nepal Agriculture Research Council (NARC) Publication Date: 2012 Pagination: 31-34 p. Languages : English (eng) Keywords: 'Puccinia', 'resistant', 'susceptible', 'rust', 'gene' Abstract: Barley is the major crop for the people living in the high hills and mountainous region of the Nepal. Rust is the major production threat for barley cultivation. However, a lot of variation can be observed on Nepalese barley accessions with respect to rust resistance characteristics. Two hundred and forty one barley accessions were screened for leaf rust ( caused by Puccinia hordei) and stripe rust ( caused by P. striformis f.sp. hordei) resistance characteristics on heading stage at Khumaltar, Lalitpur, Nepal during normal barley growing season 2011. Bonus (origin Sweden) and Local Jau (Nepalese landrace) were used as resistant and susceptible check respectively. Seventeen barley accessions showed leaf rust susceptibility but resistant with stripe rust. Thirty barley accessions showed resistant for both leaf rust and strip rust. Lower frequency of the leaf rust infection may be due to prevalent resistance gene for leaf rust resistant characteristics in Nepalese barley.
Link for e-copy: http://elibrary.narc.gov.np/?r=1490 Variation on rust resistance characteristics on Nepalese barley germplasm. [printed text] / Madan Raj Bhatta, Editor ; Resham B Amgai, Editor ; Pantha, Sumitra, Author ; Khatiwada, Shambhu P., Author . - Lalitpur : Nepal Agriculture Research Council (NARC), 2012 . - 31-34 p.
in Proceedings of the research papers presented at Special Session Marker Assisted Breeding during Working Group Meeting on Plant Breeding and Genetics from 24-25 April 2012, Khumaltar, Lalitpur, Nepal / Madan Raj Bhatta
Languages : English (eng)
Keywords: 'Puccinia', 'resistant', 'susceptible', 'rust', 'gene' Abstract: Barley is the major crop for the people living in the high hills and mountainous region of the Nepal. Rust is the major production threat for barley cultivation. However, a lot of variation can be observed on Nepalese barley accessions with respect to rust resistance characteristics. Two hundred and forty one barley accessions were screened for leaf rust ( caused by Puccinia hordei) and stripe rust ( caused by P. striformis f.sp. hordei) resistance characteristics on heading stage at Khumaltar, Lalitpur, Nepal during normal barley growing season 2011. Bonus (origin Sweden) and Local Jau (Nepalese landrace) were used as resistant and susceptible check respectively. Seventeen barley accessions showed leaf rust susceptibility but resistant with stripe rust. Thirty barley accessions showed resistant for both leaf rust and strip rust. Lower frequency of the leaf rust infection may be due to prevalent resistance gene for leaf rust resistant characteristics in Nepalese barley.
Link for e-copy: http://elibrary.narc.gov.np/?r=1490 E-copies
http://nkcs.org.np/narc/dl/?r=1490URL