An attempt was made to know the status of Paratuberculosis (Johne’s disease) a chronic granulomatous infectious disease of ruminants in Nepal. In past years intradermal skin test (Johnin test) had been conducted to identify the prevalence of the disease. Intradermal skin test carried out during 1982- 1988 in cattle of organized farms of Kathmandu and Pokhara valley showed an over all prevalence of 3.22 percent (0.9-10.13%).In 1999,115 serum samples from buffaloes and 98 serum samples from cattle of different districts of Western Nepal revealed 39 samples (33.9%) and 27(27.5%) samples positive to antibodies against paratuberculosis by absorbed ELISA……………….

An attempt was made to know the status of Paratuberculosis (Johne’s disease) a chronic granulomatous infectious disease of ruminants in Nepal. In past years intradermal skin test (Johnin test) had been conducted to identify the prevalence of the disease. Intradermal skin test carried out during 1982- 1988 in cattle of organized farms of Kathmandu and Pokhara valley showed an over all prevalence of 3.22 percent (0.9-10.13%).In 1999,115 serum samples from buffaloes and 98 serum samples from cattle of different districts of Western Nepal revealed 39 samples (33.9%) and 27(27.5%) samples positive to antibodies against paratuberculosis by absorbed ELISA……………….