Blast  (caused   by  Magnaporthe  oryzae)  is  the  major  yield   limiting  biotic  stress  for  rice farming  in  Nepal  that  is  prevalent  all  over  the  country.  Resistant variety  for  one  location becomes  susceptible  to another location  which  shows  the  need  of resistant gene  pyramiding for yield  stability. Identifying blast resistant genes  in Nepalese rice  germplasm and its use for varietal  development is very  important.  Ninety  two  Nepalese rice  accessions were  screened using 12 SSR markers  for presence of different  blast resistant genes.  IR-64  and  Sabitri  were used  as resistant check while  Masuli  and Jumli  Marshi  were  used  as susceptible check. Blast resistant genes  Pi-54,  Pi-y2(t), Pi-d(t) 1,  Pi-z,  Pi-a,  Pi-k,  Pi-yl (t)  and Pi-44  were  detected on seven,   seven,   nine,   11,  20  and  28 rice  accessions  respectively.  Eighteen  rice  accessions showed  presence Pi-b  and  Pi-g(t), 22  rice  accessions  showed  presence of Pi-29  and  Pi-11; and  two rice  accessions  showed  presence  of Pi-ta  and  Pi20(t).   Nepalese  rice  gene  pool doesn't  contain   resistant  gene  Pi-d(t)2   and  it  needs   to  incorporate from   exotic  sources. Therefore,  many  Nepalese rice  landraces  can  be  used  on  marker assisted gene  pyramiding program for development of durable  blast resistance varieties.

Blast  (caused   by  Magnaporthe  oryzae)  is  the  major  yield   limiting  biotic  stress  for  rice farming  in  Nepal  that  is  prevalent  all  over  the  country.  Resistant variety  for  one  location becomes  susceptible  to another location  which  shows  the  need  of resistant gene  pyramiding for yield  stability. Identifying blast resistant genes  in Nepalese rice  germplasm and its use for varietal  development is very  important.  Ninety  two  Nepalese rice  accessions were  screened using 12 SSR markers  for presence of different  blast resistant genes.  IR-64  and  Sabitri  were used  as resistant check while  Masuli  and Jumli  Marshi  were  used  as susceptible check. Blast resistant genes  Pi-54,  Pi-y2(t), Pi-d(t) 1,  Pi-z,  Pi-a,  Pi-k,  Pi-yl (t)  and Pi-44  were  detected on seven,   seven,   nine,   11,  20  and  28 rice  accessions  respectively.  Eighteen  rice  accessions showed  presence Pi-b  and  Pi-g(t), 22  rice  accessions  showed  presence of Pi-29  and  Pi-11; and  two rice  accessions  showed  presence  of Pi-ta  and  Pi20(t).   Nepalese  rice  gene  pool doesn't  contain   resistant  gene  Pi-d(t)2   and  it  needs   to  incorporate from   exotic  sources. Therefore,  many  Nepalese rice  landraces  can  be  used  on  marker assisted gene  pyramiding program for development of durable  blast resistance varieties.