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in
| Title : |
Genetic relationship among Nepalese rice landraces and cultivars based on rapd markers |
| Material Type: |
printed text |
| Authors: |
Bimb, Hari P., Editor ; Joshi, Bal K., Author ; David Kansakar, Author ; Ekta Ghimire, Author |
| Publisher: |
Hardinath, Janakpur : National Rice Research Programme |
| Publication Date: |
2010 |
| Pagination: |
168-184 p. |
| Keywords: |
Genetic Distance ; rice RAPD |
| Abstract: |
Genetic information of any genotype is necessary to manage and utilize them in conservation and breeding program. A total of 28
RAPD markers were used to relate the genetic structure among 50 Nepalese rice genotypes consisting of29landraces, 12 breeding
lines and 9 released cultivars. Some of them are aromatic and blast resistance. Only four primers (P41, P60, Pl09 and Pl41)
amplified the DNA of these genotypes with scorable bands. Primer 60 produced the highest number of bands (8). The highest
number of present bands (6) was shown by primer 41 in I 0 rice genotypes. Grouping of these genotypes based on the adaptation to
agro-climatic zone was not observed, probably due to low percentage coverage of genome by four primers. Most of the genotypes
grouped in two clusters. Kali Marsi and IR 24 formed separate individual cluster. Mansara and Jameli were the most similar
landraces (0.96). Churenodhan and Pranpyuri were the most closely related with Masuli. Only one genotype NR285-18 has fallen in
the first quadrant by principal component analysis and the fourth quadrant was empty. The highest contribution in PCl was from the
second band of primer 41 . This RAPD information can be used for selecting lines and for blast resistance breeding. |
| Link for e-copy: |
http://elibrary.narc.gov.np/?r=1015 |
in
Genetic relationship among Nepalese rice landraces and cultivars based on rapd markers [printed text] / Bimb, Hari P., Editor ; Joshi, Bal K., Author ; David Kansakar, Author ; Ekta Ghimire, Author . - Hardinath, Janakpur : National Rice Research Programme, 2010 . - 168-184 p. | Keywords: |
Genetic Distance ; rice RAPD |
| Abstract: |
Genetic information of any genotype is necessary to manage and utilize them in conservation and breeding program. A total of 28
RAPD markers were used to relate the genetic structure among 50 Nepalese rice genotypes consisting of29landraces, 12 breeding
lines and 9 released cultivars. Some of them are aromatic and blast resistance. Only four primers (P41, P60, Pl09 and Pl41)
amplified the DNA of these genotypes with scorable bands. Primer 60 produced the highest number of bands (8). The highest
number of present bands (6) was shown by primer 41 in I 0 rice genotypes. Grouping of these genotypes based on the adaptation to
agro-climatic zone was not observed, probably due to low percentage coverage of genome by four primers. Most of the genotypes
grouped in two clusters. Kali Marsi and IR 24 formed separate individual cluster. Mansara and Jameli were the most similar
landraces (0.96). Churenodhan and Pranpyuri were the most closely related with Masuli. Only one genotype NR285-18 has fallen in
the first quadrant by principal component analysis and the fourth quadrant was empty. The highest contribution in PCl was from the
second band of primer 41 . This RAPD information can be used for selecting lines and for blast resistance breeding. |
| Link for e-copy: |
http://elibrary.narc.gov.np/?r=1015 |
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Genetic relationship among Nepalese rice landraces and cultivars based on rapd markers
