Classification of large variation within Oryza sativa has been importance for breeders and
geneticists. A total of 39 Nepalese rice cultivars including one non-released, Fuji-102 were
classified and evaluated their characteristics features of eight isozyme systems, Alcohol
dehydrogenase (ADH), Acid phosphatase (ACP), Esterase (EST), lsocitrate dehydrogenase
(IDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Peroxidase (POX) and Shikimate
dehydrogenase (SDH). Plumules and coleoptiles of 3-5 seedlings of each cultivar were used to
extract enzyme tfSing Sodium ascorbate extraction buffer at pH 7.4. These were electrophoresed
and enzymes were detected after staining in an appropriate staining mixture. Cluster and
principal component analyses were applied on binary data matrix. Zymograms were presented
separately for each enzyme and each varietal type. A to
tal of 28, 19 and 23 distinct electromorphs were used as iso:;ymic descriptors for indica,
intermediate and japonica varietal types respectively. Multivariate analysis grouped these
cultivars into 3. These were reclassified in indica (22), intermediate (3} andjaponica (14) varietal
types. There were sixteen loci coding eight isozyme systems. All loci were f ound in indica group,
/4 loci in intermediate and 15 in japonica. Most of the enzyme systems expressed distinct
zymogramsfor indica andjaponica. Highest zymotypes were expressed by POX Loci Mdh-3 and
Sdh-1 have highest number of alleles within indica type, Pox-] within intermediate and Sdh-1 and
Pox-/ withinjaponica types. Locus Mdh-2 was not noticed in intermediate andjaponica groups.
Loci Mdh-3, Est-2 and Sdh-1 .showed hfg~est gene diversity within indica, intermediate and
j aponica rypes respectively. Among the four multilocus -enzymes, three were polymorphic. All loci
except Pox-4 were polymorhpic in intermediate varietal types. all loci were polymorhpihc in
indica and except Pox-3 and Adh:-1 all loci were polymorphic considering null allele injaponica.
This isozymic variation within and between varietal types can be itseful for further study and the
isozyme profiles of each cultivar can be used to trace their identity.

Classification of large variation within Oryza sativa has been importance for breeders and
geneticists. A total of 39 Nepalese rice cultivars including one non-released, Fuji-102 were
classified and evaluated their characteristics features of eight isozyme systems, Alcohol
dehydrogenase (ADH), Acid phosphatase (ACP), Esterase (EST), lsocitrate dehydrogenase
(IDH), Malate dehydrogenase (MDH), Malic enzyme (ME), Peroxidase (POX) and Shikimate
dehydrogenase (SDH). Plumules and coleoptiles of 3-5 seedlings of each cultivar were used to
extract enzyme tfSing Sodium ascorbate extraction buffer at pH 7.4. These were electrophoresed
and enzymes were detected after staining in an appropriate staining mixture. Cluster and
principal component analyses were applied on binary data matrix. Zymograms were presented
separately for each enzyme and each varietal type. A to
tal of 28, 19 and 23 distinct electromorphs were used as iso:;ymic descriptors for indica,
intermediate and japonica varietal types respectively. Multivariate analysis grouped these
cultivars into 3. These were reclassified in indica (22), intermediate (3} andjaponica (14) varietal
types. There were sixteen loci coding eight isozyme systems. All loci were f ound in indica group,
/4 loci in intermediate and 15 in japonica. Most of the enzyme systems expressed distinct
zymogramsfor indica andjaponica. Highest zymotypes were expressed by POX Loci Mdh-3 and
Sdh-1 have highest number of alleles within indica type, Pox-] within intermediate and Sdh-1 and
Pox-/ withinjaponica types. Locus Mdh-2 was not noticed in intermediate andjaponica groups.
Loci Mdh-3, Est-2 and Sdh-1 .showed hfg~est gene diversity within indica, intermediate and
j aponica rypes respectively. Among the four multilocus -enzymes, three were polymorphic. All loci
except Pox-4 were polymorhpic in intermediate varietal types. all loci were polymorhpihc in
indica and except Pox-3 and Adh:-1 all loci were polymorphic considering null allele injaponica.
This isozymic variation within and between varietal types can be itseful for further study and the
isozyme profiles of each cultivar can be used to trace their identity.